A5031104795- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Estrogen and related hormone effects
- Synthesis and characterization of novel inorganic/organometallic compounds
- Crystallography and molecular interactions
- Protein Kinase Regulation and GTPase Signaling
- Organometallic Complex Synthesis and Catalysis
- Amino Acid Enzymes and Metabolism
- Epigenetics and DNA Methylation
- Fluorine in Organic Chemistry
- Phosphodiesterase function and regulation
- Organoboron and organosilicon chemistry
- Neuroscience and Neuropharmacology Research
- Molecular Sensors and Ion Detection
- Inorganic Fluorides and Related Compounds
- Chemical Synthesis and Analysis
- Acute Ischemic Stroke Management
- Cancer Immunotherapy and Biomarkers
- Heat shock proteins research
- Cancer-related gene regulation
- Ferroptosis and cancer prognosis
- Coordination Chemistry and Organometallics
- Traumatic Brain Injury and Neurovascular Disturbances
- Drug Transport and Resistance Mechanisms
- Boron Compounds in Chemistry
Binghamton University
2010-2023
Sapienza University of Rome
2019-2022
Augusta University
2015-2021
Augusta University Health
2016-2020
Georgia Regents Medical Center
2015-2016
Maine Department of Education
2016
Cardiff University
1998-2012
University Hospital Schleswig-Holstein
2003-2008
University of Lübeck
2003-2008
University of Münster
1995-2005
Background Despite PD-L1 (Programmed death receptor ligand-1) expression on tumor cells and cytotoxic T lymphocytes infiltration in the microenvironment, human pancreatic cancer stands out as one of cancers that does not respond to immune checkpoint inhibitor (ICI) immunotherapy. Epigenome dysregulation has emerged a major mechanism cell exhaustion non-response ICI immunotherapy, we, therefore, aimed at testing hypothesis an epigenetic compensates function render Methods Two orthotopic mouse...
Protein N-myristoylation enables localization to membranes and helps maintain protein conformation function. N-myristoyltransferases (NMT) catalyze co- or posttranslational myristoylation of Src family kinases other oncogenic proteins, thereby regulating their In this study, we provide genetic pharmacologic evidence that inhibiting the N-myristoyltransferase NMT1 suppresses cell-cycle progression, proliferation, malignant growth prostate cancer cells. Loss abolished tumorigenic potential its...
The Alanine-Serine-Cysteine transporter ASCT2 (SLC1A5) is a membrane protein that transports neutral amino acids into cells in exchange for outward movement of intracellular acids. highly expressed peripheral tissues such as the lung and intestines where it contributes to homeostasis concentrations also plays an important role development variety cancers melanoma by transporting acid nutrients glutamine proliferating tumors. Therefore, key drug target with potentially great pharmacological...
Abstract Despite the presence of CTLs in tumor microenvironment, majority immunogenic human colon cancer does not respond to immune checkpoint inhibitor immunotherapy, and microsatellite instable (MSI) tumors are naturally eliminated. The molecular mechanism underlying inactivity tumor-infiltrating is unknown. We report here that were present both MSI stable tumors. expression H3K9me3-specific histone methyltransferase SUV39H1 was significantly elevated carcinoma compared with normal...
The neutral amino acid transporter alanine-serine-cysteine 2 (ASCT2) belongs to the solute carrier 1 (SLC1) family of transporters and transports small, acids across membrane, including physiologically important ubiquitous glutamine. Our understanding involvement ASCT2 in physiological processes involving glutamine is hampered by a lack its pharmacology absence high-affinity inhibitors. In this study, we combined an silico docking approach with experimental investigation binding parameters...
We performed proteomic studies using the GRP78 chaperone-inhibitor drug AR-12 (OSU-03012) as bait. Multiple additional chaperone and chaperone-associated proteins were shown to interact with AR-12, including: GRP75, HSP75, BAG2; HSP27; ULK-1; thioredoxin. down-regulated in situ immuno-fluorescence detection of ATP binding chaperones antibodies directed against NH2-termini but only weakly reduced central COOH portions proteins. Traditional SDS-PAGE western blotting assessment methods did not...
The acceptance of biofuels is paramount importance for the EU sustainable energy transition. This qualitative study identifies barriers and facilitators regarding biofuel technology acceptability. Stakeholders initially selected their interest influence in field were asked about four clusters variables associated with acceptability according to literature on similar technologies: per se, economic market-related aspects, political administrative social-psychological features person. Results...
Color-coded perfusion maps can be calculated from ultrasound harmonic gray-scale imaging data after contrast agent bolus injection to analyze brain tissue perfusion. First reports indicate that this method display cerebral deficits in acute ischemic stroke. We performed a prospective patient study evaluate approach.Thirty consecutive patients suffering middle artery infarction who presented our department within 12 hours symptom onset were investigated with (PHI) Levovist injection. the...
Abstract The reactions of [(Me 3 Si) CLi2thf] with Me 2 MCl (M = Al, Ga) afford the mixed trialkylmetallanes ‐CAlMe ·thf] ( 1 ) and CGaMe in high yields. coordinated THF molecule compound can be removed by sublimation vacuo to yield solvent‐free product ‐CGaMe ] ). Hydrolysis one equivalent water at 0°C gives trimeric hydroxide [{(Me CGaMe(μ‐OH)} 4 ), while reaction two equivalents room temperature yields unusually stable gallium hydroxide‐water complex CGaMe(OH)(μ‐OH)MeGaC(SiMe H O·2thf] 6...
Neutral amino acid exchange by the alanine serine cysteine transporter (ASCT)2 was reported to be electroneutral and coupled cotransport of one Na(+) ion. The cotransported sodium ion carries positive charge. Therefore, it is possible that voltage dependent. However, little information available on electrical properties ASCT2 transport process. Here, we have used a combination experimental computational approaches determine details mechanism ASCT2. [Na(+)] dependence ASCT2-associated...
Aminodimethylalanes R1R2NAlMe2 (R1 = 2,6-i-Pr2C6H3, 2,6-Me2C6H3; R2 SiMe3, Si(i-Pr)Me2, Si(t-Bu)Me2, Si(2,4,6-Me3C6H2)Me2) are prepared in high yield via reaction of the respective amine R1R2NH with trimethylaluminum n-hexane. Further (2,6-i-Pr2C6H3)N(SiMe3)AlMe2 2 equiv trimethyltin fluoride toluene affords aminoalane difluoride (2,6-i-Pr2C6H3)N(SiMe3)AlF2. An X-ray structural determination shows it to be dimeric bridging methyl and terminal amino groups. The (2,6-i-Pr2C6H3)N(SiMe3)AlF2 is...
The novel bulky alkylaluminum dihalogenides [(Me3Si)3CAlX2·THF] (X = F, 1; X Cl, 4; Br, 5; I, 6) and dialkylaluminum halogenides [(Me3Si)3CAl(Me)X·THF] 8; 9; 10) have been prepared by reaction of the mixed trialkylalane [(Me3Si)3CAlMe2·THF] with Me3SnX Cl) X2 I) in a 2:1 1:1 molar ratio, respectively. coordinated THF molecule compound 1 can be removed heating vacuo to yield trimeric solvent-free organoaluminum difluoride [{(Me3Si)3CAlF2}3] (2). Compound 2 shows no cocatalytic activity group...
Forward glutamate transport by the excitatory amino acid carrier EAAC1 is coupled to inward movement of three Na+ and one proton subsequent outward K+ in a separate step. Based on indirect evidence, it was speculated that cation binding sites bear negative charge. However, little known about electrostatics process. Valences calculated using Poisson-Boltzmann equation indicate charge transferred across membrane when only bound. Consistently, transient currents were observed response voltage...
// Laurence Booth 1 , Thomas Albers 3 Jane L. Roberts Mehrad Tavallai Andrew Poklepovic 2 Iryna O. Lebedyeva Paul Dent Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, Richmond, VA 23298, USA Medicine, Chemistry Physics, Augusta Summerville Campus, GA 30912, Correspondence to: Dent, email: pdent@vcu.edu Keywords: sorafenib, pazopanib, chaperones, ERBB, PI3K Received: April 25, 2016 Accepted: May 20, Published: 31, ABSTRACT We have recently demonstrated that...
FasL-mediated cytotoxicity is one of the mechanisms that CTLs use to kill tumor cells. However, human colon carcinoma often deregulates Fas signaling pathway evade host cancer immune surveillance. We aimed at testing hypothesis novel ceramide analogs effectively modulate function sensitize cells FasL-induced apoptosis. used rational design and synthesized twenty as modulators. Five analogs, IG4, IG7, IG14, IG17, IG19, exhibit low toxicity potent activity in sensitization Functional...
Titanosilicates with structural characteristics similar to those of many Ti-containing zeolites were synthesized from the reactions (arylamino)silanetriol [RSi(OH)3] organometallic titanium precursors [Cp*TiCl3], TiCl4·2THF, and [Cp*TiMe(μ-O)]3. The product reaction 1, is a soluble molecular silicate in which formal TiO2 fragments are trapped inside. Cp* = C5Me5; R (2,6-iPr2C6H3)NSiMe3.
We conducted this prospective study to evaluate the time course of hemorrhagic transformation (HT) and arterial recanalization in early phase ischemic stroke using transcranial sonography (TCS).Fifty-five patients with acute hemispheric <32 hours after symptom onset were studied. A 2-MHz sector probe was used brain tissue by TCS basal cerebral arteries color-coded sonography. Follow-up investigations performed up 6 days. Lesion size localization determined cranial computed tomography.Of 20...
The SLC38 family of solute transporters mediates the coupled transport amino acids and Na(+) into or out cells. structural basis for this process is not known. Here, a profile-based sequence analysis approach was used, predicting distant relationship with SLC5/6 transporter families. Homology models using LeuT(Aa) Mhp1 known structure as templates were established, location conserved binding site in center membrane helices 1 8. This homology model tested experimentally member SNAT2 by...
Substrate transport by glutamate transporters is coupled to the co-transport of 3 Na+ ions and counter-transport 1 K+ ion. The highly conserved Asp454, which may be negatively charged, interest as its side chain coordinate cations and/or contribute charge compensation. Mutation nonionizable Asn resulted in a transporter that no longer catalyzed forward transport. However, Na+/glutamate exchange was still functional, demonstrated presence transient currents following rapid substrate...
Purpose: Adolescent and young adult (AYA) cancer survivors (15-39 years) often struggle with their post-cancer identity. We developed an age-appropriate positive psychology group intervention fellow AYA in a nature-rich environment. The aim of this study was to examine the needs expectations participants new, 1-week identity intervention, evaluate experiences identify factors that contribute quality impact mental health care interventions. Methods: This mixed-method exploratory pilot...
A 9-membered triphosphorus macrocycle with o-phenylene backbone functions has been stereoselectively prepared on a CpFe(+) template by two successive nucleophilic attacks of coordinated phosphide o-fluorophenylbiphosphine.
Glutamate transporters maintain a large glutamate concentration gradient across synaptic membranes and are, thus, critical for functioning of the excitatory synapse. Mammalian concentrate inside cells through energetic coupling flux to transmembrane Na+. Structural models based on an archeal homologue, GltPh, suggest elevator-like carrier mechanism. However, determinants this carrier-based movement are not well understood. Although electrostatics play important role in governing these...