A5060274387- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Mesenchymal stem cell research
- Immune cells in cancer
- Extracellular vesicles in disease
- Gynecological conditions and treatments
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Reproductive System and Pregnancy
- Connective Tissue Growth Factor Research
- Angiogenesis and VEGF in Cancer
- Neurological Disease Mechanisms and Treatments
- Cytokine Signaling Pathways and Interactions
- Fibroblast Growth Factor Research
- Ferroptosis and cancer prognosis
- Adenosine and Purinergic Signaling
- Phagocytosis and Immune Regulation
- Sarcoma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- FOXO transcription factor regulation
- Glycosylation and Glycoproteins Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Zhujiang Hospital
2016-2024
Southern Medical University
2016-2024
And Technology Research (United Kingdom)
2022
Ministry of Education of the People's Republic of China
2022
National Clinical Research
2019-2020
Growing evidences indicate that circular RNAs (circRNAs) play an important role in the regulation of biological behavior tumor. We aim to explore circRNA glioma and elucidate how acts.Real-time PCR was used examine expression circPTN tissues normal brain (NBT). Assays dual- luciferase reporter system, biotin label RNA pull-down FISH were determine could sponge miR-145-5p miR-330-5p. Tumor sphere formation assay performed self- renewal stem cell (GSCs). Cell counting Kit-8 (CCK8), EdU flow...
The blood-brain barrier (BBB) contains tightly connected brain microvascular endothelial cells (BMECs) that hinder drug delivery to the brain, which makes tumors difficult treat. Previous studies have shown nanoparticles coated with tumor cell membranes selectively target their homologous tumors. Therefore, this study investigated whether bEnd.3-line BMEC membrane-coated poly(lactide-co-glycolide)-poly(ethylene glycol)-based doxorubicin-loaded cores (BM-PDs) can be used BMECs and cross BBB....
Abstract Background Intrauterine adhesions (IUAs) are manifestations of endometrial fibrosis characterized by inflammation and fibrinogen aggregation in the extracellular matrix (ECM). The available therapeutic interventions for IUA insufficiently effective clinical setting postoperative adhesion recurrence infertility problems. In this study, we investigated whether si-SNHG5-FOXF2 can serve as a molecular mechanism inhibition ex vivo. Methods FOXF2, TGF-β1 collagen expression levels were...
Abstract Background Exosomes are membrane-bound extracellular vesicles of 40–150 nm in size, that produced by many cell types, and play an important role the maintenance cellular homeostasis. Exosome secretion allows for selective removal harmful substances from cells. However, it remains unclear whether this process also takes place glioma Methods Herein, tumour-suppressor miR-375 was explored human Immunoblotting qRT-PCR experiments demonstrated a functional link between its target,...
Mesenchymal stem cells (MSCs) have emerged as putative therapeutic tools due to their intrinsic tumor tropism, and anti-tumor immunoregulatory properties. The limited passage self-differentiation abilities of MSCs in vitro hinder preclinical studies on them. In this study, we focused the safety immortalized mesenchymal (im-MSCs) and, for first time, studied feasibility im-MSCs candidates treatment glioma. were constructed by lentiviral transfection genes. proliferative capacity phenotype...
This paper investigated the effects of STAT3 through promoting FOXP1 transcription on proliferation, apoptosis and invasion in glioma cells.Quantitative real-time PCR (qRT-PCR) Western blot assay were administered to assess mRNA protein expression levels tissues cells, respectively. Luciferase reporter Chromatin Immunoprecipitation (ChIP) assays implemented determine correlation between FOXP1. MTT colony formation conducted identify cell growth. Flow cytometry was run detect rate cells....
Cellular internalization, delivery efficiency, and therapeutic efficacy of nanoparticles vary according to the microenvironmental complexity for tumor types. Adjusting their physicochemical properties, such as surface properties size, has significant potential dealing with complexities. Herein, we prepare four types pH-sensitive doxorubicin (DOX-D1, DOX-D2, DOX-W1, DOX-W2 Nano) using simply changing reaction medium or reactant ratio. DOX-D1 DOX-D2 Nano exhibit similar characteristics...
Gliomas—devastating intracranial tumors with a dismal outcome—are in dire need of innovative treatment. Although nanodrugs have been utilized as target therapy for certain types solid tumors, their therapeutic effects gliomas are limited due to the complications systemic circulation, blood–brain barrier (BBB), and specific glioma environment. Thus, we aimed establish nanoliposome adaptable different environments by codelivery shCD163 doxorubicin (DOX) treat gliomas. In this study, first...
Abstract Mesenchymal stem cells (MSCs) have emerged as putative therapeutic tools due to their intrinsic tumour tropism, and anti-tumour immunoregulatory properties. The limited passage self-differentiation abilities of MSCs in vitro hinder preclinical studies on them. In this study, we focused the safety immortalized mesenchymal (im-MSCs) and, for first time, studied feasibility im-MSCs candidates treatment glioma. were constructed by lentiviral transfection genes. proliferative capacity...
Abstract Background: Exosomes are membrane-bound extracellular vesicles of 40–150 nm in size, that produced by many cell types, and play an important role the maintenance cellular homeostasis. Exosome secretion allows for selective removal harmful substances from cells. However, it remains unclear whether this process also takes place glioma Methods: Herein, tumour-suppressor miR-375 was explored human Immunoblotting qRT-PCR experiments demonstrated a functional link between its target,...
Background: Glioma has the highest fatality rate among intracranial tumours. Besides, heterogeneity of gliomas leads to different therapeutic effects even with same treatment. Developing a new signature for glioma achieve concept "personalised medicine" remains significant challenge. Method: The Cancer Genome Atlas (TCGA) and Chinese (CGGA) were searched acquire information on patients. Initially, correlation univariate Cox regression analyses performed screen prognostic pyroptosis-related...
Objective To explore the effect of fibroblast growth factor receptor (FGFR) antagonist BGJ398 in growth, migration and invasiveness gliomas. Methods (1) Glioma cells U87 U251 were routinely cultured vitro divided into treatment group (10 μmol/L complete medium) control group; proliferation was detected by CCK-8 colony formation; 2 d after cultivation, invasion measured wound-healing assay Transwell assay. The phosphor-FGFR (pFGFR) level vimentin expressions Western blotting. (2) Eight...
Objective To explore the expression, role and mechanism of long non-coding RNA (lncRNA) BANCR in astrocytoma. Methods (1) Twenty-four astrocytoma tissues 6 normal brain were collected from patients accepted surgical resection conformed by pathology our hospital January 2016 to September 2017; mRNA expressions signal transduction transcriptional activator 3 (STAT3) detected real-time quantitative(qRT)-PCR; glioma dataset GSE4290 including astrocytomas downloaded analyzed web software...
Abstract Background: Exosomes are membrane-bound extracellular vesicles of 40–150 nm produced by many cell types, and playing an important role in the maintenance cellular homeostasis. Exosome secretion allows for selective removal harmful substances from cells. Whether this process also takes place glioma cells is unknown. Methods: The tumour suppressor miR-375 was explored human lines. Immunoblotting qRT-PCR experiments demonstrated a functional link between its target, connective tissue...
Abstract Background: Intrauterine adhesions (IUAs) are manifestations of endometrial fibrosis characterized by inflammation and fibrinogen aggregation in the extracellular matrix (ECM). The available therapeutic interventions for IUA insufficiently effective clinical setting postoperative adhesion recurrence infertility problems. In this study, we investigated si-SNHG5-FOXF2 can serve as a molecular mechanism inhibition ex vivo. Methods: FOXF2, TGF-β1 collagens expression levels were measure...
Abstract Background: Intrauterine adhesions (IUAs) are manifestations of endometrial fibrosis characterized by inflammation and fibrinogen aggregation in the extracellular matrix (ECM). The available therapeutic interventions for IUA insufficiently effective clinical setting postoperative adhesion recurrence infertility problems. In this study, we investigated whether si-SNHG5-FOXF2 can serve as a molecular mechanism inhibition ex vivo. Methods: FOXF2, TGF-β1 collagen expression levels were...
Abstract BACKGROUND: Mesenchymal stem cells (MSCs) have emerged as putative therapeutic tools due to their intrinsic tumour tropism, anti-tumour, and immunoregulatory properties. The limited passage self-differentiation abilities of MSCs in vitro hinder preclinical studies mesenchymal cells, makes the MSC-based treatment tumours lack a stable, uniform, homogeneous source cells. In this study, we focused on safety immortalised (im-MSCs) and, for first time, studied feasibility im-MSCs...