A5045164457- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Ocular Diseases and Behçet’s Syndrome
- Molecular Biology Techniques and Applications
- Hepatitis B Virus Studies
- RNA regulation and disease
- Viral Infections and Immunology Research
- Hepatitis C virus research
- Mycobacterium research and diagnosis
- Toxoplasma gondii Research Studies
- HIV Research and Treatment
- Biosensors and Analytical Detection
- Neonatal Health and Biochemistry
- SARS-CoV-2 detection and testing
- Liver Disease Diagnosis and Treatment
- Parvovirus B19 Infection Studies
- Pneumonia and Respiratory Infections
- SARS-CoV-2 and COVID-19 Research
- Polyomavirus and related diseases
- Periodontal Regeneration and Treatments
- Hepatitis Viruses Studies and Epidemiology
- Head and Neck Cancer Studies
Johns Hopkins University
2014-2024
Johns Hopkins Medicine
2009-2023
Columbia University Irving Medical Center
2017-2023
University of Washington Tacoma
2022
Texas Health Dallas
2022
New York Hospital Queens
2017-2021
NewYork–Presbyterian Hospital
2017-2021
Columbia University
2019
Johns Hopkins Hospital
1989-2017
Harvard University
2015-2016
A safe and effective vaccine to prevent chronic hepatitis C virus (HCV) infection is a critical component of efforts eliminate the disease.
The increase in SARS-CoV-2 infections December 2021 was driven primarily by the Omicron variant, which largely displaced Delta over a three-week span. Outcomes from infection with remain uncertain. We evaluated whether clinical outcomes and viral loads differed between during period when both variants were co-circulating.
Cytomegalovirus (CMV) retinitis is among the most common opportunistic infections in patients with AIDS and a substantial cause of visual loss. With long-term therapy, resistant CMV may develop. In prospective study 108 retinitis, 80.6% were found to have either positive blood culture or urine for at diagnosis retinitis. At 0.9% 2.7% had ganciclovir-resistant isolate isolate, respectively. Of 76 initially treated ganciclovir, 11.4% by 6 months treatment 27.5% 9 months. The development...
In Brief Background Antiviral-resistant or refractory cytomegalovirus (CMV) infection is challenging, and salvage therapies, foscarnet, cidofovir, have significant toxicities. Several investigational anti-CMV agents are under development, but more information needed on outcomes of current treatments to facilitate clinical trial design for new drugs. Methods Records solid organ transplant (SOT) hematopoietic cell (HCT) recipients at a single center over 10-year period were reviewed...
The increase in SARS-CoV-2 infections December 2021 the United States was driven primarily by Omicron variant which largely displaced Delta over a three week span. Outcomes from infection with remain uncertain. We evaluate whether clinical outcomes and viral loads differ between during period when both variants were co-circulating.
Cytomegalovirus encephalitis (CMVE) is frequently diagnosed only at postmortem because its specific clinical features have not been fully identified. We described the clinical, radiologic, and laboratory of CMVE in a retrospective review 14 autopsy-confirmed cases compared them with control group demented acquired immunodeficiency syndrome (AIDS) patients without CMVE. was more common among homosexual men, subacute onset typical (mean duration presenting symptoms 3.5 weeks versus 18...
Adenoviruses (AdV) cause diseases that range from localized, self-limited illnesses to fatal infections in immunocompromised patients. Culture is assumed be sensitive but requires viable virus and up 3 weeks for detection, it can inhibited by bacterial contamination. A new PCR method amplifying a region of the hexon gene was developed order detect AdV urine more rapidly with greater sensitivity than obtainable culture technology. All 18 serotypes tested were detected. Quantitatively,...
ABSTRACT Quantification of Epstein-Barr virus (EBV) in peripheral blood is important for the diagnosis and management serious EBV diseases, including posttransplant lymphoproliferative disorder. A variety PCR-based methods are currently use; however, there little information on their comparability. This study assessed relative performance different quantitative assays. multicenter comparative was performed at eight sites using three panels consisting serial dilutions quantified DNA extracts...
ABSTRACT Automated nucleic acid extraction is an attractive alternative to labor-intensive manual methods. We compared two automated methods, the BioRobot M48 instrument (Qiagen, Inc.) and MagNA Pure (Roche Applied Sciences) QIAamp Viral RNA Mini kit (Qiagen) TRIzol (Invitrogen), for of enterovirus RNA. Analytical sensitivity was assessed by dilution analysis poliovirus type 2 Sabin in cerebrospinal fluid. The PCR equivalent after with QIAamp, M48, Pure. All 18 replicates 100 PFU/ml were...
Viral respiratory infections are among the most common reasons for hospitalization of children in United States. Our objective was to compare molecular and conventional methods a cohort hospitalized with without symptoms viral illness (RVI).We conducted retrospective study infants toddlers between December 2007 March 2008 at Johns Hopkins Hospital. Five hundred sixty-nine 641 patient visits (89%) were tested on admission. Conventional tests (immunochromatography, direct fluorescent antibody,...
An increase in influenza like illness children and adolescents at the Johns Hopkins Health system during summer 2022 was associated with increased positivity for enterovirus/ rhinovirus. We sought to characterize epidemiology viral evolution of enterovirus D68 (EV-D68).
Cytomegalovirus (CMV) retinitis is among the most common opportunistic infections in patients with acquired immunodeficiency syndrome. In a prospective study of 210 CMV retinitis, 26 were identified as having either phenotypic or genotypic ganciclovir-resistant isolate from blood urine cultures. For culture isolates an IC50 >6.0 µm for ganciclovir, sensitivity and specificity detecting UL97 mutation 95% 98%, respectively, whereas >8.0 µM they 79% 99%, respectively. Although there trade-offs...
Cytomegalovirus (CMV) retinitis is a common opportunistic infection in patients with AIDS. With long-term therapy for CMV retinitis, resistant may develop. In prospective study of 122 2.4 and 0.8% had foscarnet-resistant blood culture isolates (50% inhibitory concentration [IC50], >400 microM) urine isolates, respectively, at diagnosis prior to treatment, whereas 4.1 6.6% cidofovir-resistant (IC50, >2 respectively. Patients were treated according best medical judgement. Of 44...
• A retrospective study of bone marrow transplant recipients shedding adenovirus type 11 in the urine was carried out to determine association between viral and hemorrhagic cystitis this population. Weekly virology surveillance cultures were obtained during first 100 days following transplantation. Adenovirus cultured from five 502 1977 through 1984. In four these patients there associated cystitis. This contrasts with an overall incidence 20% case occurred a patient Recognition origin may...
Purpose: Reports of cytomegalovirus (CMV) detection in high-grade gliomas (HGG)/glioblastoma have been conflicting. We undertook a comprehensive approach to determine the presence or absence CMV tissue, plasma, and serum HGG patients.Experimental Design: In retrospective arm, 25 fresh frozen tissues from glioblastoma patients were tested for by real-time PCR. Tissue microarrays 70 IHC 20 formalin-fixed paraffin-embedded (FFPE) chromogenic situ hybridization (CISH), targeting CMV-encoded...
Nucleotide-binding oligomerization domain 2 (NOD2) is an important innate immune sensor of bacterial pathogens. Its induction results in activation the classic NF-κB pathway and alternative pathways including type I IFN autophagy. Although importance NOD2 recognizing RNA viruses has recently been identified, its role sensing DNA not studied. We report that infection with human cytomegalovirus (HCMV) significant expression, beginning as early hours post increasing steadily 24 afterwards....
Abstract Background Our understanding of the cocirculation infrequently targeted respiratory pathogens and their contribution to symptoms during coronavirus disease 2019 (COVID-19) pandemic is currently limited. This research aims at (1) epidemiology since start pandemic, (2) assessing non-severe acute syndrome 2 (SARS-CoV-2)/influenza/respiratory syncytial virus (RSV) symptoms, (3) evaluating coinfection rates in SARS-CoV-2-positive patients, both vaccinated unvaccinated. Methods...
Prior observation has shown differences in COVID-19 hospitalization risk between SARS-CoV-2 variants, but limited information describes outcomes.Inpatients with at 5 hospitals the eastern United States were included if they had hypoxia, tachypnea, tachycardia, or fever, and variant data, determined from whole-genome sequencing local surveillance inference. Analyses stratified by history of vaccination infection. The average effect on 28-day severe disease, defined advanced respiratory...
Osteoarthritis is a degenerative joint disease that causes pain, degradation, and dysfunction. Excessive canonical Wnt signaling in osteoarthritis contributes to chondrocyte phenotypic instability loss of cartilage homeostasis; however, the regulatory niche unknown. Using temporomandibular as model multiple species, we identify Lgr5-expressing secretory cells forming inhibitory instruct Wnt-inactive chondroprogenitors form nascent synovial regulate lineage identity. Lgr5 ablation or...
Rapid (2.5- to 3.5-h) enzyme immunoassays (EIAs) for the detection of Clostridium difficile toxins have been developed. We report results simultaneous testing 700 fresh stool specimens by tissue culture cytotoxin assay and four EIAs (Bartels Prima System C. Toxin A EIA, Cambridge Biotech Cytoclone A+B Meridian Diagnostics Premier TechLab Tox-A Test EIA). In cases disagreement, culturing toxigenic was performed. total 61 (8.7%) from 46 patients were positive toxin. The sensitivity 87%, that...
The clinical significance of cytomegalovirus (CMV) foscarnet resistance was studied in patients with acquired immunodeficiency syndrome and CMV retinitis. Sequencing the pol gene performed 30 isolates. Phenotypic characterized by DNA hybridization assay (DHA) isolates plaque-reduction (PRA) 18 Nine had mutations, including V787L E756Q that were confirmed marker transfer experiments. Seven 9 a 50% inhibitory concentration (IC(50)) >600 microM DHA genotypic resistance, compared 2 21 an IC(50)...