A5071523575- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Mitochondrial Function and Pathology
- Spaceflight effects on biology
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
- Photosynthetic Processes and Mechanisms
- Autism Spectrum Disorder Research
- Obsessive-Compulsive Spectrum Disorders
- Lipid Membrane Structure and Behavior
- Advanced MRI Techniques and Applications
- Pancreatic function and diabetes
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Cellular transport and secretion
- Yeasts and Rust Fungi Studies
- Acute Myeloid Leukemia Research
- Ion channel regulation and function
- Nuclear Structure and Function
- Body Image and Dysmorphia Studies
- Hemoglobin structure and function
- Autophagy in Disease and Therapy
- Neonatal Respiratory Health Research
- Epigenetics and DNA Methylation
- Transcranial Magnetic Stimulation Studies
The First People's Hospital of Guiyang
2025
Guangdong General Hospital
2015-2024
Washington University in St. Louis
1992-2023
Pediatrics and Genetics
2023
St. Louis Children's Hospital
2019
Brown University
2013-2018
Guangdong Academy of Medical Sciences
2015-2018
Allen Institute for Brain Science
2018
University of South Florida
2016
South China University of Technology
2015
GLUT-4 is the major facilitative glucose transporter isoform in tissues that exhibit insulin-stimulated transport. Insulin regulates transport by rapid translocation of from an intracellular compartment to plasma membrane. A critical feature this process efficient exclusion membrane absence insulin. To identify amino acid domains which confer sequestration, we analyzed subcellular distribution chimeric transporters comprised and a homologous isoform, GLUT-1, found predominantly at cell...
Background:Cognitive impairment is a key feature of treatment-resistant depression (TRD) and can be related to the anterior cingulate cortex (ACC) function. Repetitive transcranial magnetic stimulation (rTMS) as an antidepressant intervention has increasingly been investigated in last two decades. However, no studies date have association between neurobiochemical changes within executive dysfunction measured TRD being treated with rTMS.
Lipins are phosphatidic acid phosphatases with a pivotal role in regulation of triglyceride and glycerophospholipid metabolism. Lipin1 is also an amplifier PGC-1α, nuclear coactivator PPAR-α responsive gene transcription. do not contain recognized membrane-association domains, but interaction these enzymes cellular membranes necessary for access to their phospholipid substrate. We identified conserved polybasic amino motif N-terminal domain previously implicated as determinant localization...
Stem cell systems are essential for the development and maintenance of polarized tissues. Intercellular signaling pathways control stem systems, where niche cells signal to maintain fate/self-renewal inhibit differentiation. In C. elegans germline, GLP-1 Notch specifies fate, employing sequence-specific DNA binding protein LAG-1 implement transcriptional response. We undertook a comprehensive genome-wide approach identify targets signaling. expected primary response target genes be evident...
Significance The Rab5 GTPase functions in early endosome (EE) fusion the endocytic pathway. Here, we propose that RAB5B also has a noncanonical vesicular function regulated secretion pathway produces mature surfactant proteins SP-B and SP-C lung. This was revealed from investigation of proband with interstitial lung disease suggestive dysfunction disorder who carried de novo Asp136His variant gene. Our modeling C. elegans provided information on genetic cell biological mechanism, analyses...
Aims/Background Patients with relapsed acute myeloid leukemia (AML) have a poor prognosis and limited treatment options. The combination of azacitidine venetoclax has demonstrated some efficacy in AML. This study aimed to investigate the effects combined on immune function quality life patients Methods retrospectively analyzed clinical data 104 AML who were admitted First People's Hospital Fuyang from January 2020 2024. divided into group (n = 53, treated azacitidine) an 51, according...
Mitochondria are highly dynamic organelles whose morphology and position within the cell is tightly coupled to metabolic function. There a limited list of essential proteins that regulate mitochondrial mechanisms govern dynamics poorly understood. However, recent evidence indicates core machinery governs linked complex intracellular signalling cascades, including apoptotic pathways, cycle transitions nuclear factor kappa B activation. Given emerging importance plasticity in pathways...
Sleep deprivation impairs learning, causes stress, and can lead to death. Notch JNK-1 pathways impact C. elegans sleep in complex ways; these have been hypothesized involve compensatory sleep. DAF-16, a FoxO transcription factor, is required for homeostatic response decreased DAF-16 loss decreases survival after bout deprivation. Here, we investigate connections between the requirement mechanical stress. Reduced function of ligand LAG-2 or kinase resulted increased time bouts during...
Abstract Sleep is evolutionarily conserved and required for organism homeostasis survival. Despite this importance, the molecular cellular mechanisms underlying sleep are not well understood. Caenorhabditis elegans exhibits sleep-like behavioral quiescence thus provides a valuable, simple model system study of regulators process. In C. elegans, epidermal growth factor receptor (EGFR) signaling in neurosecretory neuron ALA to promote after stress. We describe novel role VAV-1, guanine...
C. elegans sleep during development is regulated by genes and cellular mechanisms that are conserved across the animal kingdom (Singh et al., 2014; Trojanowski & Raizen, 2016). developmental usually assessed transition to adulthood, a 2.6 h time interval called lethargus (Raizen 2008; Singh 2011). During lethargus, animals cycle between periods of immobility (sleep bouts) active locomotion (motion bouts). Sleep bouts resemble in other species based on behavioral criteria, including cessation...
We aimed to test a combined approach identify conserved genes regulating sleep and explore the association between DNA methylation length. identified candidate associated with shorter versus longer duration in college students based on using Illumina Infinium HumanMethylation450 BeadChip arrays. Orthologous Caenorhabditis elegans were identified, we examined whether their loss of function affected C. sleep. For whose perturbation sleep, subsequently undertook small pilot study re-examine an...
In Caenorhabditis elegans, Notch signaling regulates developmentally timed sleep during the transition from L4 larval stage to adulthood (L4/A) . To identify core pathways and find genes acting downstream of signaling, we undertook first genome-wide, classical genetic screen focused on C. elegans sleep. increase efficiency, looked for mutations that suppressed inappropriate anachronistic in adult hsp::osm-11 animals overexpressing coligand OSM-11 after heat shock. We retained suppressor...
Abstract The molecular mechanisms of sleep are not fully understood. Huang et al. demonstrate that loss Caenorhabditis elegans UNC-7 or UNC-9 innexins dramatically reduces during L4/A lethargus and those partially required... An essential characteristic is heightened arousal threshold, with decreased behavioral response to external stimuli. cellular underlying threshold changes We report innexin function reduced developmentally timed in elegans. was required premotor interneurons motor...
Due to its ease of genetic manipulation and transparency, Caenorhabditis elegans (C. elegans) has become a preferred model system study gene function by microscopy. The use Aequorea victoria green fluorescent protein (GFP) fused proteins or targeting sequences interest, further expanded upon the utility C. labeling subcellular structures, which enables following their disposition during development in presence mutations. Fluorescent with excitation emission spectra different from that GFP...
Standards-based COTS (common-off-the-shelf) middleware has been shown to be effective in meeting a range of functional and QoS (quality service) requirements for distributed real-time embedded (DRE) systems. Each standard makes limiting assumptions, often implicit, about the fundamental set system capabilities constraints typical domain which applies. When characteristics particular class systems violates standard's it may appropriate modify or extent its conforming implementations better...
Abstract Rab GTPases are important regulators of intracellular vesicular trafficking. RAB5C is a member the GTPase family that plays an role in endocytic pathway, membrane protein recycling and signaling. Here we report on 12 individuals with nine different heterozygous de novo variants RAB5C. All but one patient missense (n = 9) exhibited macrocephaly, combined mild-to-moderate developmental delay. Patients loss function 2) had apparently more severe clinical phenotype refractory epilepsy...