Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, has emerged as an active mediator in many essential functions central nervous system mammals. BDNF plays significant roles neurogenesis, neuronal maturation and/or synaptic plasticity and is involved cognitive such learning memory. Despite vast literature present mammals, studies devoted to brain other animal models are scarse. Zebrafish teleost fish widely known for developmental genetic emerging model...

Abstract Reactive oxygen species (ROS) represent a by-product of metabolism and their excess is toxic for hematopoietic stem progenitor cells (HSPCs). During embryogenesis, small number HSPCs are produced from the hemogenic endothelium, before they colonize transient organ where expand, example fetal liver in mammals. In this study, we use zebrafish to understand molecular mechanisms that important caudal tissue (equivalent mammalian liver) promote HSPC expansion. High levels ROS deleterious...

Nitrogen-based fertilizers represent the most common fertilization tools, particularly used in crop food agriculture, despite low cost-efficiency and high negative environmental impact. At present, there is still inadequate information available about effects of urea on human health; nevertheless, previous studies animals observed that concentration exposure can damage different tissues, including brain. In several vertebrates, a crucial factor involved neuronal cell formation represented by...

Abstract The reparative ability of the central nervous system varies widely in animal kingdom. In mammalian brain, regenerative mechanisms are very limited and newly formed neurons do not survive longer, probably due to a non‐suitable local environment. On opposite, fish can repair brain after injury, with fast complete recovery damaged area. zebrafish, teleost used as vertebrate model, also possesses high properties injury. Taking advantage this relevant aim present study was investigate...

Hematopoietic stem cells (HSCs) guarantee the continuous supply of all blood lineages during life. In response to stress, HSCs are capable extensive proliferative expansion, whereas in steady state, largely remain a quiescent state prevent their exhaustion. DNA replication is very complex process, where many factors need exert functions perfectly concerted manner. Mini-chromosome-maintenance protein 10 (Mcm10) an important factor, required for proper assembly eukaryotic fork. this report, we...

Abstract Nerve growth factor ( NGF ), a member of the neurotrophin family, was initially described as neuronal survival and factor, but successively has emerged an active mediator in many essential functions central nervous system mammals. is synthesized precursor pro‐ cleaved intracellularly into mature . However, recent evidence demonstrates that not simple inactive precursor, also secreted outside cells can exert multiple roles. Despite vast literature present mammals, studies devoted to...

Nerve growth factor (NGF), a member of the neurotrophin family, has emerged as an active mediator in different crucial events peripheral and central nervous system. At same time, several studies showed that this can also play role non-neuronal tissues (e.g., among gonads). In spite large number present mammals, investigations devoted to NGF its receptor TrkA reproductive system other animal models, such teleost fish, are scarce. To increase our knowledge vertebrate gonads model, report...

ABSTRACT Zebrafish, a suitable and widely used teleost fish model in basic biomedical research, displays morphophysiological features of adult gonads that share some commonalities with those mammalian species. In mammals, gametogenesis is regulated, among several factors, by brain‐derived neurotrophic factor (BDNF). This neurotrophin has well‐established role the developing nervous system, as well development functions vertebrate We hypothesize BDNF also gonadal zebrafish. At this purpose,...

Evidence shows that dysfunctional SSc keratinocytes contribute to fibrosis by altering dermal homeostasis. Whether IL-25, an IL-17 family member regulating many epidermal functions, takes part in skin is unknown. Here we address the role of IL-25 fibrosis.The expression was evaluated immunofluorescence and situ hybridization 10 seven healthy donor (HD) biopsies. Epidermal equivalents (EE) reconstituted primary HD were used as a model study transcriptomic changes induced epidermis. RNA...

Abstract During development, hematopoietic stem cells (HSCs) are produced from the hemogenic endothelium and will expand in a transient niche. Prostaglandin E 2 (PGE2) is essential during vertebrate development HSC specification, but its precise source embryo remains elusive. Here, we show that zebrafish embryo, PGE2 synthesis genes expressed by distinct stromal cell populations, myeloid (neutrophils, macrophages), endothelial of caudal tissue. Ablation cells, which produce precursor...

Neurotrophins (NTs) are implicated in the maintenance and survival of peripheral central nervous systems mediate several forms synaptic plasticity. Members family include nerve growth factor (NGF), brain-derived neurotrophic (BDNF), NT3, NT4. NTs were first identified as factors for developing neurons, but pleiotropic molecules that can exert a variety functions, including regulation neuronal differentiation, axonal dendritic growth, plasticity (Bothwell, 2014). interact with two distinct...

Neurogenesis is the process by which new brain cells are formed. This crucial event emerges during embryonic life and proceeds in adulthood, it could be influenced environmental pollution. Non-combustion-derived magnetite represents a portion of coarse particulate matter (PM) contributing to air water pollution urban settings. Studies on humans have reported that other iron oxides significant damaging effects at central level, where these particles accumulate promote oxidative stress....

During early vertebrate development, hematopoietic stem and progenitor cells (HSPCs) are produced in hemogenic endothelium located the dorsal aorta, before they migrate to a transient niche where expand fetal liver caudal tissue, mammals zebrafish, respectively. In previous studies have shown that extracellular matrix (ECM) around aorta must be degraded enable HSPCs leave aortic floor reach blood circulation. However, role of ECM components HSPC specification has never been addressed. this...

Neurotrophins and their receptors are involved in the development maintenance of neuronal populations. Different reports have shown that all neurotrophin/receptor pathways can also play a role several non-neuronal tissues vertebrates, including kidney. These signaling different events to ensure correct functioning kidney, such as growth, differentiation, regulation renal tubule transport. Previous studies some fish species identified neurotrophins In this study, for first time, we compare...

Chronic exposure to heavy metals has been widely demonstrated induce pathological features in different tissues and, particular, the central nervous system. Specific neurons, including dopaminergic were observed be more susceptible toxic agents. Several previous studies performed on zebrafish (Danio rerio) models that nickel (one of most abundant metals) induces impairment memory and anxious-like behaviors. Nevertheless, this phenotypical evidence not associated with system damage, no...

In vertebrates, neurotrophins and their receptors play a fundamental role in the central peripheral nervous systems. Several studies reported that each neurotrophin/receptor signalling pathway can perform various functions during axon development, neuronal growth, plasticity. Previous investigations some fish species have identified spinal cord under physiological conditions after injuries, highlighting potential regeneration. our study, for first time, we used an excellent animal model,...

Cytoplasmic linker-associated protein-2 (CLASP2) is a member of the CLIP-associating proteins (CLASPs) family involved in structure and function microtubules Golgi apparatus. Several studies performed using different mammalian non-mammalian model organisms reported that CLASP2 controls microtubule dynamics organization networks. In Drosophila mice, an important role during development germ cell lines has been uncovered. However, no study clearly defined its fish differentiation. present...