A5085870008- Herpesvirus Infections and Treatments
- Poxvirus research and outbreaks
- Tuberculosis Research and Epidemiology
- Virus-based gene therapy research
- Mycobacterium research and diagnosis
- Diagnosis and treatment of tuberculosis
- interferon and immune responses
- Malaria Research and Control
- HIV Research and Treatment
- Microbial Community Ecology and Physiology
- RNA regulation and disease
- Genomics and Phylogenetic Studies
- Animal Virus Infections Studies
- Antenna Design and Analysis
- Leaf Properties and Growth Measurement
- Bacterial Infections and Vaccines
- Virology and Viral Diseases
- Evolution and Genetic Dynamics
- Reproductive tract infections research
- Insect Resistance and Genetics
- Polyomavirus and related diseases
- Antibiotic Resistance in Bacteria
- Crop Yield and Soil Fertility
- Vector-borne infectious diseases
- Cytokine Signaling Pathways and Interactions
University of California, Davis
2020-2024
Kansas State University
2014-2016
The University of Texas Health Science Center at Houston
2010-2011
Brigham Young University
2005-2010
Johns Hopkins University
2008
Institut de Recherche pour le Développement
2002-2007
Centre National de la Recherche Scientifique
2004-2007
University of California, Irvine
2005
Korea University
2005
Maladies Infectieuses et Vecteurs: Écologie, Génétique, Évolution et Contrôle
2004
ABSTRACT Throughout the natural course of human immunodeficiency virus (HIV) infection, follicular dendritic cells (FDCs) trap and retain large quantities particle-associated HIV RNA in follicles secondary lymphoid tissue. We have previously found that murine FDCs vivo could maintain trapped particles an infectious state for at least 9 months. Here we sought to determine whether serve as reservoir, based on criteria therein must be replication competent, genetically diverse, archival nature....
Double-stranded (ds) RNA, generated during viral infection, binds and activates the mammalian anti-viral protein kinase PKR, which phosphorylates translation initiation factor eIF2α leading to general inhibition of synthesis. Although PKR-like activity has been described in fish cells, responsible enzymes eluded molecular characterization until recent discovery goldfish zebrafish PKZ, contain Z-DNA-binding domains instead dsRNA-binding (dsRBDs). Fish amphibian PKR genes have not so far. Here...
The antiviral protein kinase R (PKR) is an important host restriction factor, which poxviruses must overcome to productively infect cells. To inhibit PKR, many encode a pseudosubstrate mimic of the alpha subunit eukaryotic translation initiation factor 2 (eIF2), designated K3 in vaccinia virus. Although interaction between PKR and eIF2α highly conserved, some orthologs from host-restricted were previously shown species-specific manner. better define this range function, we compared...
// Levi G. Ledgerwood 1,* , Dhruv Kumar Agda Karina Eterovic 2 Jo Wick 3 Ken Chen 4 Hao Zhao Loubna Tazi 5 Pradip Manna 6 Spencer Kerley Radhika Joshi 1 Lin Wang 7 Simion I. Chiosea James David Garnett Terance Ted Tsue Jeremy Chien 8,9 Gordon B. Mills Jennifer Rubin Grandis 10,11 and Sufi Mary Thomas 1,9,12 Department of Otolaryngology, University Kansas Medical Center, Cancer City, MO, USA Systems Biology Bioinformatics, MD Anderson Houston, TX, Biostatistics, Computational Biology,...
Plasmodium vivax causes the most geographically widespread human malaria, accounting annually for 70-80 million clinical cases throughout tropical and subtropical regions of world's continents. We have analyzed DNA sequences Csp (circumsporozoite protein) gene in 24 representative strains P. 2 simium , which parasitizes several species New World monkeys. The are two types, VK210 VK247, differ by three diagnostic amino acid replacements, one each 5′ 3′ terminal [5′ nonrepeat (NR) NR] an...
Myxoma virus (MYXV) causes localized cutaneous fibromas in its natural hosts, tapeti and brush rabbits; however, the European rabbit, MYXV lethal disease myxomatosis. Currently, molecular mechanisms underlying this increased virulence after cross-species transmission are poorly understood. In study, we investigated interaction between M156 host protein kinase R (PKR) to determine their crosstalk with proinflammatory nuclear factor kappa B (NF-κB) pathway. Our results demonstrated that...
Translational control of transcription factor ATF4 through paired upstream ORFs (uORFs) plays an important role in eukaryotic gene regulation. While it is typically induced by phosphorylation eIF2α, translation can be also expression a translational inhibitor protein, eIF5-mimic protein 1 (5MP1, known as BZW2) mammals. Here we show that the 5MP maintained eukaryotes under strong purifying selection, but uniquely missing two major phyla, nematoda and ascomycota. The common function from...
Gonorrhea is a major sexually transmitted disease (STD) in many countries worldwide. The emergence of fluoroquinolone resistance has complicated efforts to control and treat this disease. We report the first study evolutionary processes acting on transmission dynamics resistant gonococcal population from Shanghai, China. compare these findings with our previous evolution sensitive Baltimore, MD.Ninety six samples were collected male patients All resistant. Seven MLST housekeeping genes, two...
Abstract The antiviral protein kinase R (PKR) is activated by viral double-stranded RNA and phosphorylates translation initiation factor eIF2α, thereby inhibiting virus replication. Most poxviruses contain two PKR inhibitors, called E3 K3 in vaccinia (VACV), which are determinants of host range. prevailing model for function that it inhibits through the non-specific sequestration (ds) RNA. Our data revealed Syrian hamster was resistant to E3, at odds with model. However, still sensitive...
ABSTRACT Although lower-resource countries have by far the highest burden of tuberculosis, knowledge Mycobacterium tuberculosis population structure and genetic diversity in these regions remains almost nonexistent. In this paper, 150 Moroccan M. isolates circulating Casablanca were genotyped random amplified polymorphic DNA analysis using 10 different primers mycobacterial interspersed repetitive units-variable number tandem repeats typing at 12 loci. The tests revealed a basically clonal...
Tuberculosis (TB) represents a global public health threat and is leading cause of morbidity mortality worldwide. Effective control TB complicated with the emergence multidrug resistance. Yet, there fundamental gap in understanding complex dynamic interactions between different
ABSTRACT The antiviral protein kinase R (PKR) is activated by viral double-stranded RNA and phosphorylates translation initiation factor eIF2α, thereby inhibiting virus replication. Most poxviruses contain two PKR inhibitors, called E3 K3 in vaccinia (VACV), which are determinants of host range. prevailing model for function that it inhibits through the non-specific sequestration (ds) RNA. Our data revealed Syrian hamster was resistant to E3, at odds with model. However, still sensitive...
Replicate experiments are often difficult to find in evolutionary biology, as this field is inherently an historical science. However, viruses, bacteria and phages provide opportunities study evolution both natural experimental contexts, due their accelerated rates of short generation times. Here we investigate HIV-1 by using a model represented monozygotic twins infected synchronically at birth with population from shared blood transfusion source. We explore the processes dynamics that...
Yaba monkey tumor virus (YMTV) and Tanapox (TPV) are members of the Yatapoxvirus genus can infect humans other primates. Despite threat posed by yatapoxviruses, factors determining their host range poorly understood. In this study, we analyzed ability YMTV TPV orthologs vaccinia K3 (called 012 in TPV), which share 75% amino acid identity with one another, to inhibit PKR from 15 different primate species. We first used a luciferase-based reporter, found that inhibited species-specific manner...
Yaba monkey tumor virus (YMTV) and Tanapox (TPV) are members of the Yatapoxvirus genus can infect humans other primates. Despite threat posed by yatapoxviruses, factors determining their host range poorly understood. In this study, we analyzed ability YMTV TPV orthologs vaccinia K3 (called 012 in TPV), which share 75% amino acid identity with one another, to inhibit PKR from 15 different primate species. We first used a luciferase-based reporter, found that inhibited species-specific manner...
ABSTRACT Tuberculosis (TB) represents a global public health threat and is the leading cause of morbidity mortality worldwide. Effective control TB complicated with emergence multidrug resistance. Yet, there still fundamental gap in understanding complex dynamic interactions between different Mycobacterium tuberculosis strains host. In this project, we investigated host immune response to M. strains, including avirulent or virulent rifampin-resistant isoniazid-resistant THP-1 cells. We...