A5101807372- Cancer-related molecular mechanisms research
- Autism Spectrum Disorder Research
- Genomics and Chromatin Dynamics
- Ion Channels and Receptors
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Biochemical Analysis and Sensing Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Biosensing Techniques and Applications
- Neuroscience of respiration and sleep
- Child Development and Digital Technology
- Genetics and Neurodevelopmental Disorders
- Molecular Biology Techniques and Applications
- Receptor Mechanisms and Signaling
- Simulation Techniques and Applications
- Heme Oxygenase-1 and Carbon Monoxide
- Real-time simulation and control systems
- Circular RNAs in diseases
- Gene expression and cancer classification
- 14-3-3 protein interactions
- Autophagy in Disease and Therapy
- PI3K/AKT/mTOR signaling in cancer
- Genetic Syndromes and Imprinting
Ningxia Medical University
2024-2025
Nanjing Medical University
2017-2024
Ningxia University
2024
Peking Union Medical College Hospital
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Urumqi 4th People's Hospital
2021
The Fourth People's Hospital
2021
China Tobacco
2020
Johns Hopkins Medicine
1991-2018
Johns Hopkins University
1991-2018
Nitric oxide (NO) and carbon monoxide (CO) seem to be neurotransmitters in the brain. The colocalization of their respective biosynthetic enzymes, neuronal NO synthase (nNOS) heme oxygenase-2 (HO2), enteric neurons altered intestinal function mice with genomic deletion enzymes ( nNOS Δ/Δ HO2 ) suggest neurotransmitter roles for CO nervous system. We now establish that are both interact as cotransmitters. Small smooth muscle cells from depolarized, apparent additive effects double knockouts /...
Abstract Background Many of the most popular pre-processing methods for Affymetrix expression arrays, such as RMA, gcRMA, and PLIER, simultaneously analyze data across a set predetermined arrays to improve precision final measures expression. One problem associated with these algorithms is that measurements particular sample are highly dependent on samples used normalization results obtained by different may not be comparable. A related an organization producing and/or storing large amounts...
Carbon monoxide (CO) is proposed as a physiological messenger. CO activates cGMP and has direct effect on potassium channels. Both actions of lead to hyperpolarization cell's resting membrane potential, suggesting that may function hyperpolarizing factor, although evidence still lacking. Here we take advantage the known potential gradient exists in muscle layers gastrointestinal tract determine whether an endogenous factor. We find heme oxygenase-2-null mice have depolarized smooth cells gut...
Induction of glutathione transferases (EC. 2.5.1.18), NAD(P)H:(quinone-acceptor) oxidoreductase (EC 1.6.99.2; quinone reductase) and other detoxification enzymes is a major mechanism for protecting cells against the toxicities electrophiles, including many carcinogens. Although inducers these two belong to different chemical classes, they nevertheless contain (or acquire by metabolism) electrophilic centres that appear be essential inclusive activity, are Michael reaction acceptors [Talalay,...
Abstract Background A major challenge in computational biology is to extract knowledge about the genetic nature of disease from high-throughput data. However, an important obstacle both biological understanding and clinical applications "black box" decision rules provided by most machine learning approaches, which usually involve many genes combined a highly complex fashion. Achieving biologically relevant results argues for different strategy. promising alternative base prediction entirely...
This study aimed to characterize PANoptosis-related genes with immunoregulatory features in osteoarthritis (OA) and investigate their potential diagnostic therapeutic implications. Gene expression data from OA patients healthy controls were obtained the Expression Omnibus (GEO) database. Differential analysis functional enrichment conducted identify (PRGs) associated pathogenesis. A model was developed using LASSO regression, value of key PRGs evaluated Receiver Operating Characteristic...
Abstract Transcriptional repressor GATA binding 1 (TRPS1), an atypical transcription factor, functions as a transcriptional and is also implicated in human cancers. However, the underlying mechanism of TRPS1 contributing to malignancy remains obscure. In current study, we report that recognizes both gene proximal distal start site (TSS) sequences repress expression. Co-IP mass spectrometry biochemical studies showed binds CHD4/NuRD(MTA2). Genome-wide molecular revealed CHD4/NuRD(MTA2)...
The delta 5-3-ketosteroid isomerase (EC 5.3.3.1) of Pseudomonas testosteroni catalyzes the conversion androst-5-ene-3,17-dione to androst-4-ene-3,17-dione by a stereospecific transfer 4 beta-proton 6 beta-position. reaction involves two steps: (a) rate-limiting concerted enolization, comprising protonation 3-carbonyl oxygen phenolic hydroxyl group Tyr-14 and abstraction carboxylate Asp-38, (b) rapid reketonization dienol, which may or not be concerted. active-site mutant D38N, lacks base...
Gonorrhea is a major sexually transmitted disease (STD) in many countries worldwide. The emergence of fluoroquinolone resistance has complicated efforts to control and treat this disease. We report the first study evolutionary processes acting on transmission dynamics resistant gonococcal population from Shanghai, China. compare these findings with our previous evolution sensitive Baltimore, MD.Ninety six samples were collected male patients All resistant. Seven MLST housekeeping genes, two...
Abstract Background Autism is a complex disease involving both environmental and genetic factors. Recent efforts have implicated the correlation of genomic imprinting brain development in autism, however pathogenesis autism not completely clear. Here, we used bioinformatic tools to provide comprehensive analysis autism-related genes, imprinted genes spatially temporally differentially expressed human brain, aiming explore relationship between imprinting. Methods This study analyzed...
Abstract Protein Kinase D (PKD) family contains PKD1, PKD2, and PKD3 in human. Compared to consistent tumor‐suppressive functions of PKD1 breast cancer, how PKD2/3 cancer are not fully understood. In the current study, we found that PKD2 but were preferentially overexpressed involved regulating cell proliferation metastasis. Integrated phosphoproteome, transcriptome, interactome showed was associated with multiple cancer‐related pathways, including adherent junction, regulation actin...
Background Radiofrequency ablation (RFA) is a widely used treatment for atrial fibrillation, reducing the risk of cardiac arrhythmia. Detailed visualization and quantification scarring has potential to improve preprocedural decision-making postprocedural prognosis. Conventional bright-blood late gadolinium enhancement (LGE) MRI can help detect scars; however, its suboptimal myocardium blood contrast inhibits accurate scar estimation. Purpose To develop test free-breathing LGE approach that...
Fungal secondary metabolites serve as a rich resource for exploring lead compounds with medicinal importance. Diorcinol N (DN), fungal metabolite isolated from an endophytic fungus, Arthrinium arundinis, exhibits robust anticancer activity. However, the mechanism of DN remains unclear. In this study, we examined growth-inhibitory effect on different human cancer cell lines. We found that decreased viability A3 T-cell leukemia cells in time- and concentration-dependent manner. Transcriptome...
Milk production traits play an important role in dairy cattle breeding, and single nucleotide polymorphisms can be used as effective molecular markers for milk trait marker-assisted breeding cattle. Based on the results of preliminary GWAS, candidate genes
Fundic tone is maintained through a balance of excitatory and inhibitory input to fundic smooth muscle. The aim this study was determine the role serotonin (5-HT) 5-HT receptors in modulating murine tone. Muscle strips were prepared from fundus. Intracellular recordings made circular muscle cells, effects on junction potentials evoked by electrical field stimulation (EFS) determined. induced concentration-dependent contraction depolarization that tetrodotoxin resistant. 1B/D receptor...
Somatic mutations in 3'-untranslated regions (3'UTR) do not alter amino acids and are considered to be silent cancers. We found that such can promote tumor progression by altering microRNA (miRNA) targeting efficiency consequently affecting miRNA⁻mRNA interactions. identified 67,159 somatic located the 3'UTRs of messenger RNAs (mRNAs) which interactions (functional mutations, funcMutations), 69.3% these funcMutations (the degree energy change > 12 kcal/mol) were significantly loss miRNA-mRNA...
Abstract Background Genomic localized hypermutation regions were found in cancers, which reported to be related the prognosis of cancers. This genomic is quite different from usual somatic mutations frequency occurrence and density. It like a “violent storm”, just what Greek word “kataegis” means. Results There are needs for light-weighted simple-to-use toolkit identify visualize genome. Thus we developed R package meet these needs. The used only three steps regions, i.e., i) read variation...
Reduced fasting or postprandial gastric volumes have been implicated in the pathophysiology of functional dyspepsia. The mechanisms that underlie control fundic volume are incompletely understood, partly because an inability to accurately measure vivo small animals. Small animals useful models evaluate mechanisms, e.g., knockout aim this study was determine whether ultrasonometric technique monitors contraction and relaxation mice effect modulation cholinergic, nitrergic, serotonergic...
MicroRNAs (miRNAs) are a class of evolutionarily conserved small noncoding RNAs, ~22 nt in length, and found diverse organisms play important roles the regulation mRNA translation degradation. It was shown that miRNAs were involved many key biological processes through regulating expression targets. Genetic polymorphisms miRNA target sites may alter therefore result alterations drug Recent studies have demonstrated SNPs can affect efficiency. However, there still large number specific...
The human major histocompatibility complex (MHC), also known as leukocyte antigen (HLA), plays an important role in the adaptive immune system by presenting non-self-peptides to T cell receptors. MHC region has been shown be associated with a variety of diseases, including autoimmune organ transplantation and tumours. However, structural analytic tools HLA are still sparse compared number identified alleles, which hinders disclosure its pathogenic mechanism.To provide integrative analysis...