A5070521305- Virus-based gene therapy research
- Poxvirus research and outbreaks
- Herpesvirus Infections and Treatments
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Viral Infections and Vectors
- Bacterial Infections and Vaccines
- Virology and Viral Diseases
- Plant Virus Research Studies
- Microbial infections and disease research
- Thermal Regulation in Medicine
- Animal Virus Infections Studies
- Respiratory Support and Mechanisms
- Asthma and respiratory diseases
- Eosinophilic Disorders and Syndromes
- HIV Research and Treatment
- interferon and immune responses
- Rabies epidemiology and control
- Cancer Mechanisms and Therapy
- Spine and Intervertebral Disc Pathology
- Anesthesia and Sedative Agents
- Diverse Topics in Contemporary Research
- Otolaryngology and Infectious Diseases
- Peptidase Inhibition and Analysis
- Animal Nutrition and Physiology
University of California, Davis
2019-2024
Northwestern University
2022-2023
Soonchunhyang University Hospital
2017-2022
Ajou University Hospital
2020
Hallym University
2015-2019
Kyungpook National University
2018-2019
Abstract Background Community–acquired pneumonia (CAP) is one of the leading worldwide causes childhood morbidity and mortality. Its disease burden varies by age etiology time dependent. We aimed to investigate annual seasonal patterns in etiologies pediatric CAP requiring hospitalization. Methods conducted a retrospective study 30,994 children (aged 0–18 years) with between 2010 2015 at 23 nationwide hospitals South Korea. Mycoplasma pneumoniae (MP) was clinically classified as...
Several bat species act as asymptomatic reservoirs for many viruses that are highly pathogenic in other mammals. Here, we have characterized the functional diversification of protein kinase R (PKR), a major antiviral innate defense system. Our data indicate PKR has evolved under positive selection and undergone repeated genomic duplications bats contrast to all studied mammals single copy gene. Functional testing relationship between poxvirus antagonists revealed how an evolutionary conflict...
The antiviral protein kinase R (PKR) is an important host restriction factor, which poxviruses must overcome to productively infect cells. To inhibit PKR, many encode a pseudosubstrate mimic of the alpha subunit eukaryotic translation initiation factor 2 (eIF2), designated K3 in vaccinia virus. Although interaction between PKR and eIF2α highly conserved, some orthologs from host-restricted were previously shown species-specific manner. better define this range function, we compared...
ABSTRACT The life cycle of hepatitis C virus (HCV) is highly dependent on host cellular proteins for propagation. In order to identify the factors involved in HCV propagation, we performed protein microarray assay using nonstructural 5A (NS5A) as a probe. Of ∼9,000 human immobilized microarray, approximately 90 were identified NS5A interactors. these candidates, Pim1, member serine/threonine kinase family composed three different isoforms (Pim1, Pim2, and Pim3), was selected further study....
Poxviruses are unusual DNA viruses that replicate in the cytoplasm. To do so, they encode approximately 100 immunomodulatory proteins counteract cytosolic nucleic acid sensors such as cGAMP synthase (cGAS) along with several other antiviral response pathways. Yet most of these immunomodulators expressed very early infection while many variable host range determinants, and significant gaps remain our understanding poxvirus sensing evasion strategies. Here, we show after is established,...
Abstract Double‐stranded RNA‐activated protein kinase R (PKR) is an important and rapidly evolving antiviral kinase. Most poxviruses contain two distinct PKR inhibitors, called E3 K3 in vaccinia virus (VACV), the prototypic orthopoxvirus. prevents homodimerization by binding double‐stranded RNA, while acts as a pseudosubstrate inhibitor directly to activated thereby inhibiting interaction with its substrate eIF2α. In our study here, we analyzed orthologs from phylogenetically capripoxviruses...
Abstract The antiviral protein kinase R (PKR) is activated by viral double-stranded RNA and phosphorylates translation initiation factor eIF2α, thereby inhibiting virus replication. Most poxviruses contain two PKR inhibitors, called E3 K3 in vaccinia (VACV), which are determinants of host range. prevailing model for function that it inhibits through the non-specific sequestration (ds) RNA. Our data revealed Syrian hamster was resistant to E3, at odds with model. However, still sensitive...
Abl is a central regulator of multiple cellular processes controlling actin dynamics, proliferation, and differentiation. Here we showed that knockdown impaired hepatitis C virus (HCV) propagation. Treatment tyrosine kinase-specific inhibitor, imatinib dasatinib, also significantly decreased HCV RNA protein levels in HCV-infected cells. We both dasatinib selectively inhibited infection at the entry step life cycle, suggesting kinase activity may be necessary for entry. Using pseudoparticle...
Hepatitis C virus (HCV) is a major cause of chronic liver disease and highly dependent on cellular proteins for propagation. To identify the factors involved in HCV propagation, we recently performed protein microarray assays using nonstructural 5A (NS5A) as probe. Of 90 candidates, selected soluble resistance-related calcium-binding (sorcin) further characterization. Sorcin expressed certain cancer cells. We verified that NS5A interacted with sorcin through domain I NS5A, phosphorylation...
Receptor for activated C kinase 1 (RACK1) is a small ribosomal subunit protein that phosphorylated by vaccinia virus (VacV) to maximize translation of postreplicative (PR) mRNAs harbor 5' polyA leaders. However, RACK1 multifunctional both controls directly and acts as scaffold signaling from the ribosome. This includes stress ribosome-associated quality control (RQC) ribotoxic response (RSR) pathways. As VacV infection activates RQC signaling, whether influences viral synthesis through its...
Background . The quantification of asthma medication reduction and its relation to an aggravation during pregnancy at individual level are unclear. Methods We conducted a nationwide retrospective cohort study asthmatic pregnant women in South Korea. All the medications were ranked from 1 4 according guideline-based stepwise approach. assessed daily sums ranks their association with exacerbations three phases based on individual’s delivery date: before, during, after pregnancy. Results...
ABSTRACT The antiviral protein kinase R (PKR) is activated by viral double-stranded RNA and phosphorylates translation initiation factor eIF2α, thereby inhibiting virus replication. Most poxviruses contain two PKR inhibitors, called E3 K3 in vaccinia (VACV), which are determinants of host range. prevailing model for function that it inhibits through the non-specific sequestration (ds) RNA. Our data revealed Syrian hamster was resistant to E3, at odds with model. However, still sensitive...
Vaccinia virus (VACV) was instrumental in eradicating variola (VARV), the causative agent of smallpox, from nature. Since its first use as a vaccine, VACV has been developed vector for therapeutic vaccines and an oncolytic virus. These applications take advantage VACV's easily manipulated genome broad host range outstanding platform to generate recombinant viruses with variety applications. Several methods have VACV, including marker selection transient dominant selection. Here, we present...
Two trials were carried out to assess the effects of different ratios standardized ileal digestible isoleucine:lysine (SID Ile:Lys) on growth performance broiler chickens fed low-protein diets. A total 1,320 male distributed in each trial into 6 treatments, with 10 replicates 22 birds each. control diet was formulated that satisfied nutritional requirements broilers, and a reduced protein content, meeting requirements, except for SID Ile levels. Five Ile:Lys (56%, 61%, 66%, 71%, 76%)...
Abstract Hepatitis C virus (HCV) is the major causative agent of chronic liver diseases, including cirrhosis and hepatocellular carcinoma. The recent development highly effective direct-acting antivirals (DAAs) has revolutionized treatment HCV patients. However, these DAAs are exorbitantly expensive for majority patients worldwide. Moreover, drugs still show genotypic difference in cure rate have some resistant-associated variants. Tylophorine, a natural compound derived from Tylophora...
Awareness during general anesthesia occurs in approximately 0.1-0.2% of cases; nevertheless, particular attention is required because it can lead to critical complications including insomnia, depression, anxiety, and post-traumatic stress disorder. To prevent these complications, bispectral index (BIS) end-tidal anesthetic gas (ETAG) concentration monitoring are commonly used examine patient consciousness surgery. In the present case, an 80-year-old man was scheduled for total gastrectomy....
Abstract Background The study was designed to evaluate the ability of a novel electronic automatic notification system (EANS) detect significant changes in transfusion‐associated vital signs (VSs) during transfusion and determine whether EANS improved acute reaction (ATR) detection rates suspected ATR reporting rates. Study Design Methods VSs were measured three times per unit or batch product transfused:—before, 15 minutes after commencement, at completion transfusion—and recorded on EANS....
Purpose: Croup is known to have epidemics in seasonal and biennial trends, be strongly associated with of parainfluenza virus.However, annual croup not been clearly reported Korea.This study aimed examine the seasonal/annual patterns etiologies childhood Korea during a consecutive 6-year period.Methods: Pediatric data were collected from 23 centers 1 January 2010 31 December 2015.Electronic medical records, including multiplex reverse transcription polymerase chain reaction (RT-PCR) results,...
Vaccinia virus (VACV) was instrumental in eradicating variola (VARV), the causative agent of smallpox, from nature. Since its first use as a vaccine, VACV has been developed vector for therapeutic vaccines and an oncolytic virus. These applications take advantage VACV's easily manipulated genome broad host range outstanding platform to generate recombinant viruses with variety applications. Several methods have VACV, including marker selection transient dominant selection. Here, we present...
Yaba monkey tumor virus (YMTV) and Tanapox (TPV) are members of the Yatapoxvirus genus can infect humans other primates. Despite threat posed by yatapoxviruses, factors determining their host range poorly understood. In this study, we analyzed ability YMTV TPV orthologs vaccinia K3 (called 012 in TPV), which share 75% amino acid identity with one another, to inhibit PKR from 15 different primate species. We first used a luciferase-based reporter, found that inhibited species-specific manner...
Yaba monkey tumor virus (YMTV) and Tanapox (TPV) are members of the Yatapoxvirus genus can infect humans other primates. Despite threat posed by yatapoxviruses, factors determining their host range poorly understood. In this study, we analyzed ability YMTV TPV orthologs vaccinia K3 (called 012 in TPV), which share 75% amino acid identity with one another, to inhibit PKR from 15 different primate species. We first used a luciferase-based reporter, found that inhibited species-specific manner...
Purpose: A fire in the city, classified as a social disaster, does huge damage to life and property. In Particular, threat is being increased by concentrated population density, increase number of buildings complex land use according deepening urbanization.Therefore, it important prevent advance observing golden time for suppression, rescue first-aid activities. However, issue illegal parking continues be discussed one factors that hinder dispatch trucks, private vehicles Seoul expected...