Determining the levels of human mitochondrial heteroplasmy is utmost importance in several fields. In spite this, there are currently few published works that have focused on this issue. order to increase knowledge DNA (mtDNA) heteroplasmy, main goal work investigate frequency and mutational spectrum mtDNA genome. To address a set nine primer pairs designed avoid co-amplification nuclear (nDNA) sequences origin (NUMTs) was used amplify genome 101 individuals. The analysed individuals...

We analyzed the control region of mitochondrial DNA (mtDNA) from maternally related individuals originating Azores Islands (Portugal) in order to estimate mutation rate mtDNA and gain insights into process by which a new arises segregates heteroplasmy. Length and/or point heteroplasmies were found at least one individual 72% studied families. Eleven substitutions found, all them heteroplasmy, five appear be somatic mutations six can considered germinal, evidencing high frequency healthy...

Previous genetic, anthropological and linguistic studies have shown that Roma (Gypsies) constitute a founder population dispersed throughout Europe whose origins might be traced to the Indian subcontinent. Linguistic evidence point Indo-Aryan ethnic groups from North-western India as ancestral parental of Roma. Recently, strong genetic hint supporting this theory came study private mutation causing primary congenital glaucoma. In present study, complete mitochondrial control sequences...

To date, there are no published primers to amplify the entire mitochondrial DNA (mtDNA) that completely prevent amplification of nuclear (nDNA) sequences origin. The main goal this work was design, validate and describe a set primers, specifically sequence complete human mtDNA, allowing correct interpretation mtDNA heteroplasmy in healthy pathological samples. Validation performed using two different approaches: (i) Basic Local Alignment Search Tool (ii) isolated nDNA obtained from sperm...

Objectives: To determine the African, European and Native-American paternal contributions in genetic samples from Department of Bolivar (Colombia) with aims establishing (1) possible population substructures, (2) proportion biological African heritage admixed populations European, Amerindian, descent.Methods: Y-SNPs were typed six communities, including Palenque (renowned for its linguistic cultural heritage).Results: Findings reveal a high diversity Y-haplogroups. With exception Palenque,...

Abstract Telomeric DNA is composed of highly conserved sequences which are present at the termini chromosomes as well intrachromosomal locations. Here, we studied a Chinese hamster ovary (CHO) cell line, BL‐10, with stable amplified telomeric We show that or interstitial sites in this line and another CHO HA‐1, radiosensitive they more prone to breakage than would be expected based on percentage genome sequences. The frequency 4.3 8.3 times higher overall. These conclusions reached by both...

The non-recombining nature of the Y chromosome and well-established phylogeny Y-specific Single Nucleotide Polymorphisms (Y-SNPs) make them useful for defining haplogroups with high geographical specificity; therefore, they are more apt than Y-STRs to detect population stratification in admixed populations from diverse continental origins. Different Y-SNP typing strategies have been described address issues history movements within geographic territories interest. In this study, we...

Abstract A new human genome reference sequence – GRCh37 was recently generated and made available by the Genome Reference Consortium. Since prior disposable hg18 previously used for mitochondrial DNA primer BLAST validation, a revision of those published pairs is required. Thus, aim this Short Communication to perform an in silico test nine using report pertinent modifications. The analysis showed that one tested requires revision. Therefore, validated pair, which specifically amplifies...

Within the opisthobranchs, cephalaspideans are traditionally considered a transitional group between typical testacean prosobranchs and shell-less opisthobranchs. The cephalaspidean anatomy, including presence of cephalic shield, is related to burrowing through soft sediment. Recent studies have shown that some herbivorous carnivorous contain secondary metabolites. micro-herbivorous bubble snails Bullidae Haminoeidae families known metabolites which different ecological roles....

Taste perception is crucial in monitoring food intake and, hence, thought to play a significant role human evolution. To gain insights into possible adaptive signatures genes encoding bitter, sweet, and umami taste receptors, we surveyed the available sequence variation data from 1000 Genomes Project Phase 3 for TAS1R (TAS1R1-3) TAS2R (TAS2R16 TAS2R38) families. Our study demonstrated that these two families have experienced contrasting evolutionary histories: While TAS1R1 TAS1R3 showed...

The Ibizan Hound is a canine breed native to the Mediterranean region, where leishmaniasis an endemic zoonosis. Several studies indicate low prevalence of this disease in dogs, whereas other breeds present high prevalence. However, underlying molecular mechanisms still remain unknown. aim work analyse relationship between serum levels cytokines and genomic profiles two breeds, (resistant model) Boxer (susceptible model).In study, we haplotypes genes encoding related immune response...

Abstract Several studies have shown the importance of recent events in configuration genetic landscape a specific territory. In this context, due to phenomena repopulation and demographic fluctuations that took place centuries, Iberian Northern plateau is very interesting case study. The main aim work check if population movements together with existing boundaries (geographical administrative) influenced current composition area. To accomplish general purpose, mitochondrial DNA variations...

Alleles at the TPOX STR locus have 6-14 different numbers of a four-nucleotide (AATG) repeat motif arranged in tandem. Although tri-allelic genotypes are generally rare, pattern has higher frequency, varying widely among populations. Despite this, there few accurate reports to disclose nature third allele. In this work we present data obtained from 45 individuals belonging same pedigree, which cases genotypes. The subjects were apparently healthy with normal biological development. We...

Abstract Objectives We examined internal lineages and haplotype diversity in Portuguese samples belonging to J‐M304 improve the spatial temporal understanding of introduction this haplogroup Iberia, using available knowledge about phylogeography its main branches, J1‐M267 J2‐M172. Methods A total 110 males descent were analyzed for 17 Y‐chromosome bi‐allelic markers seven short tandem repeats (Y‐STR) loci. Results Among individuals ( n = 36), five different sub‐haplogroups identified, with...

Human mitochondrial DNA (mtDNA) has been extensively used in population and evolutionary genetics studies. Thus, a valid estimate of human mtDNA rate is important many research fields. The small number estimations performed for the coding region molecule, showed differences between phylogenetic empirical approaches. We analyzed portion (tRNALeu, ND1 tRNAIle genes), using individuals belonging to extended families from Azores Islands (Portugal) with main aim providing mutation under different...

A simple bioassay system was developed to study locomotory and ovipositional responses of screwworm, Cochliomyia hominivorax (Coquerel), flies bovine blood inoculated with eight species coliform bacteria that were isolated from screwworm-infested animal wounds. When exposed odors bacteria-inoculated which incubated for 72 h at 37°C, ≈50% 7- 10-d-old gravid females landed on the by end 15 min test exposure. Only 17% 7-d-old reproductively sterile (from irradiated pupae) previtellogenic...

Recent genomic analyses suggest that the current North African gene pool was mainly influenced by population flow coming from East altered genetic structure of autochthonous Berber populations. Such has not been extensively addressed yet using populations Middle-eastern origin as reference.To discern component in background Tunisian Arabs and evaluate extent Middle into populations.This study examined 113 Tunisians well-known Arabian Kairouan region, 15 autosomal Short Tandem Repeats (STRs)...

Larvae of screwworm, Cochliomyia hominivorax (Coquerel), were reared on diets solidified with an inexpensive starch-grafted sodium polyacrylate gel (brown and white formulations) a control diet Water-Lock G-400 gel, the standard gelling agent used for larval in Screwworm Eradication Program (of United States Department Agriculture collaboration government Mexico). Mean pupal weight, number pupae, percent emergence, sex ratio, adult longevity, fecundity, fertility recorded from test brown...

Journal Article A New Meatless Diet for Adult Screwworm (Diptera: Calliphoridae) Get access M. F. Chaudhury, Chaudhury Research Laboratory, USDA-ARS, Apartado 544, Tuxtla Gutierrez, Chiapas, Mexico Search other works by this author on: Oxford Academic PubMed Google Scholar L. Alfredo Alvarez, Alvarez Lopez Velazquez of Economic Entomology, Volume 93, Issue 4, 1 August 2000, Pages 1398–1401, https://doi.org/10.1603/0022-0493-93.4.1398 Published: 01 2000