Abstract CRISPR/Cas9-mediated beta-globin ( HBB ) gene correction of sickle cell disease (SCD) patient-derived hematopoietic stem cells (HSCs) in combination with autologous transplantation represents a recent paradigm therapy. Although several Cas9-based -correction approaches have been proposed, functional vivo erythropoiesis has not investigated previously. Here, we use humanized globin-cluster SCD mouse model to study Cas9-AAV6-mediated HBB- HSCs within the context transplantation. We...

Abstract The membrane TNF-α is known to serve as a precursor of the soluble form TNF-α. Although it has been reported biological functions ligand, outside-to-inside (reverse) signal transmitted through poorly understood. Here we report novel function mediated by via into cells expressing Activation anti-TNF-α Ab against on human T cell leukemia virus (HTLV) I-infected line, MT-2, or PHA-activated normal CD4+ resulted in induction an adhesion molecule, E-selectin (CD62E), with peak 12–24 h,...

To investigate whether a polymorphism(s) or mutation(s) in the tumor necrosis factor receptor II (TNFRII) gene is involved pathogenesis of systemic lupus erythematosus (SLE).All 10 exons TNFRII were analyzed by exon-specific polymerase chain reaction-single-strand conformation polymorphism, followed nucleotide sequencing that displayed aberrant bands. analyze function polymorphisms, full-length complementary DNA each allele was transfected HeLa cells and then studied for specific binding...

The bacterial transposon Tn3 encodes a site-specific recombination system. requires the product of tnpR, gene previously identified as repressor transposase. This is site specific and takes place somewhere within sequence C-G-A-A-A-T-A-T-T-A-T-A-A-A-T-T-A-T-C but at least one additional outside this. phenotype mutations in this system suggests that transposition proceeds by mechanism which cointegrates are intermediates.

Bladder rupture can occur as a late complication of radiation therapy targeting the pelvic organs. It is often diagnosed peritonitis caused by intraperitoneal urine leakage. We report case an internal hernia and strangulated ileus bladder wall defect that occurred due to rupture, which served orifice. The patient was woman in her 70s who had undergone radical hysterectomy postoperative for cervical cancer 37 years prior. She with underwent emergency surgery abdominal pain. Intraoperative...

In the 13 years since their discovery, prokaryotic insertion sequences (IS) have changed from a scientific curiosity (Jordan et al. 1967; Malamy Saedler and Starlinger 1967a,b) to potent tool for bacterial geneticists (Kleckner 1975; Botstein Kleckner 1977; Chumley 1979). Their significance in evolution is now well established: Insertion cousins, transposons (Tn), promote rapid by providing plasticity genome via deletions rearrangements. They account emergence of new pathogenic bacteria...

Membrane TNF-alpha is a precursor form of soluble and exerts pro-inflammatory functions in cell-to-cell contact manner. We showed that membrane induced upon activation on the cell surface CD4+ T cells CD8+ cells. In patients with systemic lupus erythematosus (SLE), percentage TNF-alpha-bearing (41.5+/-12.3%) was significantly higher compared those healthy controls (26.7+/-3.9%) (p=0.007) or rheumatoid arthritis (29.8+/-15.4%) (p=0.038). from SLE displayed cytotoxic activity against L929 It...

To determine predictive factors of response to interferon (IFN) therapy in chronic hepatitis C patients, we administered IFN-α, 6 million U, intramuscularly daily for 2 consecutive weeks, then three times a week, 136 patients judged have virus (HCV) infection according HCV-RNA positivity. We also investigated the most effective length IFN-α treatment efficacy factors, i.e., histological activity index, genotype, and levels. Patients were classified either into short-term group (entire period...

Abstract CRISPR/Cas9-mediated beta-globin ( HBB ) gene correction of Sickle Cell Disease (SCD) patient-derived hematopoietic stem cells (HSCs) in combination with autologous transplantation represents a novel paradigm therapy. Although several Cas9-based -correction approaches have been proposed, functional vivo erythropoiesis has not investigated. Here, we used humanized globin-cluster SCD mouse model to study Cas9-AAV6-mediated HSCs within the context transplantation. We discover that...

Cardiopulmonary resuscitation (CPR) can sometimes induce organ injury, however, such an occurrence is rare. We herein report a case of liver injury due to CPR with life-threatening pulmonary embolization (PE) that required the patient undergo surgical hemostasis and antithrombotic therapy.A woman in her 70s fell off bicycle. She suffered cardiopulmonary arrest underwent CPR. was diagnosed PE catheter treatment anticoagulant therapy; blood pressure did not increase. Contrast-enhanced computed...

A 31-year-old woman was admitted because of persistent remittent fever. Tricuspid valve endocarditis due to Staphylococcus aureus identified as the cause The patient had no history intravenous drug abuse, oral contraceptives or predisposing cardiac disease. Huge hepatomegaly found without any signs congestive heart failure. Liver enzyme abnormalities were not detected throughout entire course therapy. liver biopsy specimen revealed peliosis hepatis. Treatment with panipenem/betamipron...

Behçet's disease is a multisystem inflammatory disorder with unknown etiology. It has been shown that the titer of plasma complement component 9 (C9) good indicator activity. Therefore, involvement C9 in pathogenesis suggested. We report case associated complete deficiency (C9D) carrying homozygous nonsense mutation at Arg-95 (R95X). The patient presented typical characteristics disease, such as uveitis, recurrent oral aphthae and genital ulcers, arthritis, suggesting does not play an...

Rib fractures can cause injury to some organs. We herein report a case of hemorrhagic shock due intercostal artery that occurred during initial trauma care (ITC) treated by resuscitative thoracotomy (RT) and transcatheter arterial embolization (TAE) with multiple displaced rib (RFs) traumatic head (THI). A man in his 50s who was injured traffic accident transferred our institution helicopter for emergency medical treatment. He underwent left thoracic drainage on site. On admission, he...

Behçet's disease is a multisystem inflammatory disorder with unknown etiology. It has been shown that the titer of plasma complement component 9 (C9) good indicator activity. Therefore, involvement C9 in pathogenesis suggested. We report case associated complete deficiency (C9D) carrying homozygous nonsense mutation at Arg-95 (R95X). The patient presented typical characteristics disease, such as uveitis, recurrent oral aphthae and genital ulcers, arthritis, suggesting does not play an...