A5044166109- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Immune cells in cancer
- Graphene and Nanomaterials Applications
- Immunotherapy and Immune Responses
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- Bone Tissue Engineering Materials
- Polymer Surface Interaction Studies
- Infection Control in Healthcare
- Gold and Silver Nanoparticles Synthesis and Applications
- Extracellular vesicles in disease
- Cancer Research and Treatments
- Multiple and Secondary Primary Cancers
- Genetic factors in colorectal cancer
- Tissue Engineering and Regenerative Medicine
- Workplace Violence and Bullying
- Gastric Cancer Management and Outcomes
- Allergic Rhinitis and Sensitization
- Nanomaterials for catalytic reactions
- Cancer Diagnosis and Treatment
- Pesticide Residue Analysis and Safety
- Mesenchymal stem cell research
- Dental Research and COVID-19
- Field-Flow Fractionation Techniques
Massachusetts General Hospital
2022-2023
University of Toronto
2013-2022
Canada Research Chairs
2021-2022
Toronto East General Hospital
2016
State Key Laboratory of Food Science and Technology
2014
Jiangnan University
2014
Brigham and Women's Hospital
2012-2013
Harvard University
2012-2013
Harvard–MIT Division of Health Sciences and Technology
2012-2013
Harvard Stem Cell Institute
2012-2013
Understanding how nanoparticles are eliminated from the body is required for their successful clinical translation. Many promising nanoparticle formulations in vivo medical applications large (>5.5 nm) and nonbiodegradable, so they cannot be renally. A proposed pathway these hepatobiliary elimination, but transport has not been well-studied. Here, we explored barriers that determined elimination of through route. The route usually following pathway: (1) liver sinusoid, (2) space Disse, (3)...
Significance Nanomaterials are developed for treating and diagnosing cancer, but only 0.7% (median) delivered to a solid tumor. To address this delivery problem, we examining each biological barrier determine its impact on tumor delivery. Because the liver sequesters up 70% of nanomaterials, in study, asked, if Kupffer cells were removed, what is delivery? While demonstrate that increased 150 times, achieved 2% nanomaterials different size, material, type. This suggests need focus...
A significant challenge to delivering therapeutic doses of nanoparticles targeted disease sites is the fact that most become trapped in liver. Liver-resident macrophages, or Kupffer cells, are key cells hepatic sequestration nanoparticles. However, precise role macrophage phenotype plays nanoparticle uptake unknown. Here, we show human modulates hard uptake. Using gold nanoparticles, examined by monocyte-derived macrophages had been driven a "regulatory" M2 an "inflammatory" M1 and found...
Lymph node follicles capture and retain antigens to induce germinal centers long-lived humoral immunity. However, control over antigen retention has been limited. Here we discovered that conjugated nanoparticle carriers of different sizes impacts the intralymph transport specific cell interaction. We found follicular dendritic (FDC) networks determine follicle fate these nanoparticles by clearing smaller ones (5-15 nm) within 48 h retaining larger (50-100 for 5 weeks. The 50-100 nm-sized had...
Biodegradable elastomers synthesized under mild conditions with highly tunable mechanical properties are described. These elastomeric biomaterials biocompatible, exhibit minimal deformation following cyclical tensile loading, and permit tight control over the release kinetics of encapsulated bioactive molecules. As a service to our authors readers, this journal provides supporting information supplied by authors. Such materials peer reviewed may be re-organized for online delivery, but not...
The successful delivery of nanoparticles to solid tumors depends on their ability pass through blood vessels and into the tumor microenvironment. Here, we discovered a subset endothelial cells that facilitate nanoparticle transport tumors. We named these (N-TECs). show only 21% located small number are involved in transporting N-TECs have an increased expression genes related vessel permeability compared other cells. act as gatekeepers determine entry point, distribution, cell accessibility, enter
Nanoparticles need to navigate a complex microenvironment target cells in solid tumors after extravasation. Diffusion is currently the accepted primary mechanism for nanoparticle distribution tumors. However, extracellular matrix can limit diffusion. Here, we identified tumor-associated macrophages as another key player transporting and redistributing nanoparticles tumor microenvironment. We found actively migrate toward extravasated from vessels, engulfing them stroma. The carry 2–5 times...
Three-dimensional (3D) optical microscopy can be used to understand and improve the delivery of nanomedicine. However, this approach cannot performed for analyzing liposomes in tissues because processing step make transparent imaging typically removes lipids. Here, we developed a tag, termed REMNANT, that enables 3D organic materials biological tissues. We demonstrated utility tag mapping intact also showed is able monitor release entrapped therapeutic agents. found their cargo >100-fold...
This field study aimed to determine the incidence and distribution of needlestick injuries among medical trainees at a community teaching hospital in Toronto, Canada.The was performed during 2013-2015 academic years Toronto East General Hospital (TEGH), University Toronto-affiliated community-teaching years. Eight-hundred forty trainees, including students, residents, post-graduate fellows, were identified invited via email participate an anonymous online fluidsurveys.com survey 16...
There is a tremendous focus on the application of nanomaterials for treatment cancer. Nonprimate models are conventionally used to assess biomedical utility nanomaterials. However, these animals often lack an intact immunological background, and tumors in do not develop spontaneously. We introduce preclinical woodchuck hepatitis virus-induced liver cancer model as platform nanoparticle (NP)-based vivo experiments. Liver development out-bred occurs result persistent viral infection, mimicking...
The delivery of therapeutic nanoparticles to target cells is critical their effectiveness. Here we quantified the impact biological barriers on macrophages in two different tissues. We compared gold liver versus those tumor. found that nanoparticle tumor was 75% less than due structural barriers. tumor-associated took up more Kupffer absence Our results highlight cellular targets.
The use of tissue adhesives for internal clinical applications is limited due to a lack materials that balance strong adhesion with biocompatibility. substrate topography explored reduce the volume highly reactive and toxic glue without compromising adhesive strength. Micro‐textured patches coated thin layer cyanoacrylate achieve similar levels employing large amounts adhesive, superior level achieved when coating applied non‐textured patch. In vivo studies demonstrate reduced inflammation...
43 Background: Immunotherapy (IO) has shown remarkable efficacy in gastrointestinal (GI) cancers with high microsatellite instability (MSI-H). We evaluated real world outcomes of patients treated neoadjuvant IO for MSI-H colorectal (CRC) and gastroesophageal (GE) cancers. Methods: queried diagnoses, pathology reports, clinic notes receiving at Massachusetts General Hospital from October 2014 to March 2022 using the Research Patient Data Registry MATLAB. 1140 were identified esophageal,...