ZIF-8 MOFs, with their large specific surface area and void volume, unique biodegradability pH sensitivity, significant loading capacity, have been widely used as carrier materials for bioactive molecules such drugs, vaccines genes.

Inadequate antigen capture and insufficient antigen-presenting cell (APC) activity at tumor sites limit the effectiveness of in situ vaccines. To address this, poly(glutamic acid-cholinephosphate) (pGluCP) is introduced as a polymer with membrane adhesion properties capable capturing both water-soluble insoluble antigens from necrotic cells while recruiting more APCs. The approach uses manganese-mineralized black phosphorus (MnBP) coated pGluCP αPD-1 antibodies to create MnBP@pGluCP-αPD-1...

Dendritic cells (DCs)-based tumor vaccines have the advantages of high safety and rapid activation T cells, been approved for clinical treatment. However, conventional DC some severe problems, such as poor DCs in vitro, low level antigen presentation, reduced cell viability, difficulty targeting lymph nodes vivo, resulting therapeutic effects. In this research, magnetic nanoparticles Fe

Single-walled carbon nanotubes (SWCNTs) have been used to deliver single-stranded (ssDNA). ssDNA in oligonucleotide can act as an inhibitor of microRNA regulate cellular functions. However, these are difficult bind with low transferring efficiency cells. To this end, we designed regulatory and functional units form ssDNA-SWCNT hybrids study their binding effects efficiency. The unit on mimics the (MI) miRNA-382, which plays a crucial role progress many diseases such renal interstitial...

Tumor vaccines can inhibit or eliminate tumors by vaccinating hosts with tumor antigens to activate antigen-specific immune responses and have gained wild attention. However, their clinical application efficacy is often comprised due the low safety poor efficiency of vaccine adjuvants/carriers. Specifically, adjuvants/carriers usually could not efficiently recruit antigen presenting cells (APCs) capture directly damage these cells. Therefore, ideal should effectively APCs be friendly for...

Background: Although several advancements have been achieved in research and treatment of lung adenocarcinoma the past few years, mechanism concerning cancerous cell migration cause chemoresistance remains ambiguous. This aimed to explore impact miR-1247-3p adenocarcinoma. Methods: The mRNA expression STAT5A were conducted with qRT-PCR. Lentiviral vectors containing mimics inhibitors constructed. Cell examined using Transwell assay. To observe chemotherapy resistance, Docetaxel, Doxorubicin,...

CaCO3particles, due to their unique properties such as biodegradation, pH-sensitivity, and porous surface, have been widely used carrier materials for delivering drugs, genes, vaccines, other bioactive molecules. In these applications, CaCO3particles are often administered intravenously. this sense, the interaction between blood components plays a key role in delivery efficacy biosafety, though hemocompatibility of has not evaluated until now. Deficiency biosafety information delayed...

Abstract Tumor vaccines, a crucial immunotherapy, have gained growing interest because of their unique capability to initiate precise anti-tumor immune responses and establish enduring memory. Injected tumor vaccines passively diffuse the adjacent draining lymph nodes, where residing antigen-presenting cells capture present antigens T cells. This process represents initial phase response constitutes pivotal determinant effectiveness. Nevertheless, granularity paradox, arising from different...

This article reports the characterization of two siblings diagnosed with late-onset multiple Acyl-CoA dehydrogenase deficiency (MADD) caused by mutations in electron transfer flavoprotein(ETF)-ubiquinone oxidoreductase (ETF-QO) (ETFDH) gene. Whole exome sequencing (WES) was performed proband's pedigree. Clinical phenotypes Proband 1 (acidosis, hypoglycemia, hypotonia, muscle weakness, vomiting, hepatomegaly, glutaric acidemia, and aciduria) were consistent symptoms MADD ETFDH mutation....

Abstract Background Marfan syndrome (MFS) is a rare autosomal dominant connective tissue disorder affecting the cardiovascular, skeletal, and ophthalmic systems. This report aimed to describe novel genetic background treatment prognosis of MFS. Case presentation A proband was initially diagnosed with bilateral pathologic myopia suspected We performed whole exome sequencing found pathogenic nonsense FBN1 mutation in proband, which confirmed diagnosis Notably, we identified second SDHB ,...

The purpose of this study was to elucidate the biological role MicroRNA-223 (miRNA-223) in mediating malignant progression esophageal cancer and underlying mechanism. MiRNA-223 levels were measured using qRT-PCR 50 paired tissues adjacent paracancerous tissues. correlation between miRNA-223 level pathological indicators patients analyzed. In vitro experiments assessed impact on proliferative, migratory, invasive abilities cells. Additionally, rescue conducted investigate involvement its...

Our study investigated miR-222’s effect on NSCLC apoptosis and proliferation. H1650 cells were assigned into group, SI group IN followed by analysis of Akt-mTOR protein content, cell proliferation, miR-222 expression Western blot, Hoechst 33258 fluorescence staining, qRT-PCR, cck-8, immunohistochemistry was lowest in highest ( P < 0.05). The nucleus shrinkage rate significantly higher than that 0.05) with showed more had OD value having p-Akt/p-mTOR lower level number positive while...