Cancer immunotherapy holds significant promise for addressing diverse malignancies. Nevertheless, its efficacy remains constrained by the intricate tumor immunosuppressive microenvironment. Herein, a light-triggered nanozyme Fe-TCPP-R848-PEG (Fe-MOF-RP) was designed remodeling The Fe-TCPP-MOFs were utilized not only as core catalysis component against destruction but also biocompatible delivery vector of an immunologic agonist, improving long circulation and enrichment. Concurrently, it...

Oxidative damage to the kidneys is a primary factor in occurrence of kidney stones. This study explores inhibitory effect Porphyra yezoensis polysaccharides (PYP) on oxalate-induced renal injury by detecting levels oxidative damage, expression adhesion molecules, and intracellular organelles revealed molecular mechanism biology methods. Additionally, we validated role PYP vivo using crystallization model hyperoxalate-induced rats. effectively scavenged overproduction reactive oxygen species...

Vascular smooth muscle cell damage is a key step in inducing vascular calcification that yields hydroxyapatite (HAP) as major product. The effect of the shape HAP on to cells has yet be investigated. In this study, we compared differences toxicity four various morphological nano-HAP crystals, namely, H-Rod, H-Needle, H-Sphere, and H-Plate, rat aortic (A7R5). sizes these crystals were 39 nm × 115 nm, 41 ×189 56 91 192 respectively. Results showed all HAPs decreased viability, disorganized...

Inadequate antigen capture and insufficient antigen-presenting cell (APC) activity at tumor sites limit the effectiveness of in situ vaccines. To address this, poly(glutamic acid-cholinephosphate) (pGluCP) is introduced as a polymer with membrane adhesion properties capable capturing both water-soluble insoluble antigens from necrotic cells while recruiting more APCs. The approach uses manganese-mineralized black phosphorus (MnBP) coated pGluCP αPD-1 antibodies to create MnBP@pGluCP-αPD-1...

Kidney stones, which have high prevalence and recurrence rates, often cause severe oxidative damage inflammation. The ultrasmall hydrodynamic diameter of nanoparticles is crucial for their enrichment in the kidneys to exert biological activity. However, integrating crystallization inhibition therapeutic functions into a single nanoparticle challenging. A novel iron (Fe)-gallic acid (Ga) metal-phenolic networks (Fe-Ga MPNs) developed treating calcium oxalate (CaOx) nephropathies. These MPNs...

ZIF-8 MOFs, with their large specific surface area and void volume, unique biodegradability pH sensitivity, significant loading capacity, have been widely used as carrier materials for bioactive molecules such drugs, vaccines genes.

Schematic Illustration of the Preparation SOM-ZIF-8-based Antigen Delivery System and Its Role in Enhancing Cross-presentation Cellular Immunity for Cancer Immunotherapy.

Abstract The interaction between nanohydroxyapatite (HAP) and smooth muscle cells is an important step in vascular calcification. However, the effect of shape HAP on adhesion endocytosis to aortic has been rarely reported. Four different morphological crystals (H‐Rod, H‐Needle, H‐Sphere, H‐Plate) were selected interact with rat (A7R5). Fluorescence‐labeled was used detect crystal then pretreated endocytic inhibitors explore pathway endocytotic crystals. distribution inside outside...

Dendritic cells (DCs)-based tumor vaccines have the advantages of high safety and rapid activation T cells, been approved for clinical treatment. However, conventional DC some severe problems, such as poor DCs in vitro, low level antigen presentation, reduced cell viability, difficulty targeting lymph nodes vivo, resulting therapeutic effects. In this research, magnetic nanoparticles Fe

Kidney stone is a urinary system disease with high incidence, and effective drugs are limited. Hydroxycitric acid (HCA), mainly found in Garcinia cambogia Hibiscus sabdariffa, can reduce body weight calcium salt deposition. In this study, we explored the molecular mechanisms of HCA inhibition renal oxalate (CaOx) deposition nephrotoxicity an oxalate-induced cell model rat model. could effectively inhibit CaOx crystal adhesion oxidative damage. Further cellular transcriptomic analysis vitro...

Renal epithelial cell injury and cell-crystal interaction are closely related to kidney stone formation.This study aims explore the inhibition of endocytosis nano-sized calcium oxalate monohydrate (nano-COM) crystals protection corn silk polysaccharides (CCSPs) with different carboxyl contents (3.92, 7.75, 12.90, 16.38%). The nano-COM protected or unprotected by CCSPs were co-cultured human renal proximal tubular cells (HK-2), then changes in biochemical indicators detected.CCSPs could...

Background: The damage to renal tubular epithelial cells is closely related the formation of kidney stones. At present, research on drugs that can protect from remains limited. Methods: This study aims explore protective effects four different sulfate groups (-OSO3-) Laminaria polysaccharides (SLPs) human proximal (HK-2) and determine difference in endocytosis nano-sized calcium oxalate monohydrate (COM) crystals before after protection. COM with a size 230 ± 80 nm was used HK-2 establish...

<italic>Porphyra yezoensis</italic> polysaccharide could effectively reduce the cell damage and osteogenic transformation of vascular smooth muscle cells induced by hydroxyapatite crystals.

Tumor vaccines can inhibit or eliminate tumors by vaccinating hosts with tumor antigens to activate antigen-specific immune responses and have gained wild attention. However, their clinical application efficacy is often comprised due the low safety poor efficiency of vaccine adjuvants/carriers. Specifically, adjuvants/carriers usually could not efficiently recruit antigen presenting cells (APCs) capture directly damage these cells. Therefore, ideal should effectively APCs be friendly for...

CaCO3particles, due to their unique properties such as biodegradation, pH-sensitivity, and porous surface, have been widely used carrier materials for delivering drugs, genes, vaccines, other bioactive molecules. In these applications, CaCO3particles are often administered intravenously. this sense, the interaction between blood components plays a key role in delivery efficacy biosafety, though hemocompatibility of has not evaluated until now. Deficiency biosafety information delayed...

Immune vaccination is a critically important strategy in disease prevention and treatment. In vaccination, efficient vaccine carriers are necessary to augment the immune response initiated by vaccines generally with weak immunogenecity. Nowadays, commercially available vaccine/carrier formulations induce effective humoral immunity but or none cellular immunity. However, practice, rather than plays key role treating some intractable diseases such as AIDS cancers, which elicited cytotoxic T...

Abstract Tumor vaccines, a crucial immunotherapy, have gained growing interest because of their unique capability to initiate precise anti-tumor immune responses and establish enduring memory. Injected tumor vaccines passively diffuse the adjacent draining lymph nodes, where residing antigen-presenting cells capture present antigens T cells. This process represents initial phase response constitutes pivotal determinant effectiveness. Nevertheless, granularity paradox, arising from different...

<title>Abstract</title> Calcium salt deposition in the kidney induces epithelial-to-mesenchymal transition (EMT) renal tubular epithelial cells, which is pathological basis for progression to fibrosis patients with stones; however, effective drugs prevent and treat this disease have not been adequately investigated. In study, we conducted a comprehensive analysis of fibrosis-related core genes by utilizing bioinformatics on RNA-seq data, along web database information. Additionally, designed...