A5006947389- Molecular Sensors and Ion Detection
- Advanced biosensing and bioanalysis techniques
- Luminescence and Fluorescent Materials
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Nanocluster Synthesis and Applications
- Molecular Junctions and Nanostructures
- Click Chemistry and Applications
- Cancer, Hypoxia, and Metabolism
- Organic Light-Emitting Diodes Research
- Dendrimers and Hyperbranched Polymers
- Ubiquitin and proteasome pathways
- Crystallography and molecular interactions
- Nanoplatforms for cancer theranostics
- DNA and Nucleic Acid Chemistry
- Advanced Proteomics Techniques and Applications
- Trace Elements in Health
- Porphyrin and Phthalocyanine Chemistry
- N-Heterocyclic Carbenes in Organic and Inorganic Chemistry
- Lanthanide and Transition Metal Complexes
- Synthetic Organic Chemistry Methods
- Metal complexes synthesis and properties
- Extracellular vesicles in disease
- Biotin and Related Studies
- Photochromic and Fluorescence Chemistry
University of Hong Kong
2013-2024
Hong Kong Science and Technology Parks Corporation
2023-2024
Chinese University of Hong Kong
2023
University of California, Berkeley
2017-2022
Novartis (Switzerland)
2018
University of California System
2017
Institute of Molecular Functional Materials
2012-2017
University Grants Committee
2012-2017
Berkeley College
2017
State Key Laboratory of Synthetic Chemistry
2016
Targeted protein degradation has arisen as a powerful strategy for drug discovery allowing the targeting of undruggable proteins proteasomal degradation. This approach most often employs heterobifunctional degraders consisting protein-targeting ligand linked to an E3 ligase recruiter ubiquitinate and mark interest One challenge with this approach, however, is that only few recruiters currently exist targeted applications, despite hundreds known ligases in human genome. Here, we utilized...
The interaction of conjugated polyelectrolyte, PPE-SO3–, with platinum(II) complexes, [Pt(tpy)(C≡CC6H4−CH2NMe3-4)](OTf)2 (1) and [Pt(tpy)(C≡C–CH2NMe3)](OTf)2 (2), has been studied by UV–vis, steady-state time-resolved emission spectroscopy. A unique FRET from PPE-SO3– to the aggregated complex 1 on polymer chain Pt···Pt demonstrated, resulting in growth triplet metal-metal-to-ligand charge transfer (3MMLCT) near-infrared (NIR) region. This two-component ensemble employed a...
Palladium(ii) complexes supported by tetradentate [N^C^C^N] and [O^N^C^N] ligand systems display sky blue to red phosphorescence with emission quantum yields lifetimes up 0.64 272 μs, respectively. Femtosecond time-resolved fluorescence (fs-TRF) measurements on these Pd(ii) reveal a fast intersystem crossing from singlet triplet manifolds time constants of 0.6-21 ps. DFT/TDDFT calculations revealed that, as result the spiro-fluorene bridging tertiary amine units ligands, T
Significance Copper is a required metal nutrient for life, yet its altered homeostasis associated with many diseases. Thus, to develop new methods help decipher copper biology, we present an activity-based ratiometric fluorescence resonance energy transfer (FRET) probe that exploits biomimetic, copper(I)-dependent cleavage reaction enable imaging of loosely bound, labile pools in cells and oxidation state selectivity self-calibrating response. Application this technology cellular models...
Abstract Palladium(II) complexes are generally reactive toward substitution/reduction, and their biological applications seldom explored. A new series of palladium(II) N‐heterocyclic carbene (NHC) that stable in the presence thiols reported. representative complex, [Pd(C^N^N)(N,N′‐nBu 2 NHC)](CF 3 SO ) ( Pd1 d , HC^N^N=6‐phenyl‐2,2′‐bipyridine, N,N′‐nBu NHC=N,N′‐di‐n‐butylimidazolylidene), displays potent killing activity cancer cell lines (IC 50 =0.09–0.5 μ m but is less cytotoxic a normal...
Excited state properties, photo-catalysis and cellular imaging of photo-stable bis-NHC Ir(<sc>iii</sc>) complexes are described.
Water-soluble alkynylplatinum(II) terpyridine complexes, [Pt{tpy(C6H4CH2NMe3-4)-4′}(CC–Ar)](OTf)2 [Ar = C6H3–(OH)2-3,5 (1), C6H4OH-4 (2), C6H3–(OMe)2-3,5 (3)], have been synthesized and characterized. The photophysical electrochemical properties of the complexes studied. Complex 1 has found to undergo aggregation at low pHs, leading metal–metal and/or π–π interactions emergence a triplet metal-metal-to-ligand charge transfer (3MMLCT) emission in near-infrared (NIR) region, intensity which...
Hydrogen peroxide (H2O2) is a central reactive oxygen species (ROS) that contributes to diseases from obesity cancer neurodegeneration but also emerging as an important signaling molecule. We now report versatile histochemical approach for detection of H2O2 can be employed across broad range cell and tissue specimens in both healthy disease states. have developed first-generation H2O2-responsive analogue named Peroxymycin-1, which based on the classic cell-staining molecule puromycin enables...
In hepatocellular carcinoma (HCC) patients with extrahepatic metastasis, the lung is most frequent site of metastasis. However, how microenvironment favors disseminated cells remains unclear. Here, it found that nidogen 1 (NID1) in metastatic HCC cell-derived extracellular vesicles (EVs) promotes pre-metastatic niche formation by enhancing angiogenesis and pulmonary endothelial permeability to facilitate colonization tumor EV-NID1 also activates fibroblasts, which secrete necrosis factor...
The end-to-end aggregation of gold nanorods (GNRs) has been demonstrated to be directed by a thioacetate-containing alkynylplatinum(II) terpyridine complex. in situ deprotected complex is preferentially attached at the ends and induce GNRs an "end-to-end" manner Pt···Pt π-π interactions, which have characterized electron microscopy, energy dispersed X-ray (EDX) analysis, UV-vis absorption spectroscopy. assembly into chain-like nanostructures can controlled concentration Pt(II) complexes.
Copper is a required nutrient for life and particularly important to the brain central nervous system. Indeed, copper redox activity essential maintaining normal physiological responses spanning neural signaling metabolism, but at same time misregulation associated with inflammation neurodegeneration. As such, chemical probes that can track dynamic changes in spatial resolution, especially loosely bound, labile forms, are valuable tools identify characterize its contributions healthy disease...
Amphiphilic gold(<sc>iii</sc>) porphyrin–PEG conjugates can undergo self-assembly into nanostructures, resulting in promising anti-cancer properties with minimal systemic toxicity. The nanostructures be employed as nanocarriers for drug co-delivery.
Hypoxia, low oxygen (O2), is a key feature of all solid cancers, including hepatocellular carcinoma (HCC). Genome-wide CRISPR-Cas9 knockout library screening used to identify reliable therapeutic targets responsible for hypoxic survival in HCC. We find that protein-tyrosine phosphatase mitochondrial 1 (PTPMT1), an important enzyme cardiolipin (CL) synthesis, the most significant gene and ranks just after hypoxia-inducible factor (HIF)-1α HIF-1β as crucial survival. CL constitutes membrane...
Abstract Cysteine has been exploited as the binding site of covalent drugs. Its high sensitivity to oxidation is also important for regulating cellular processes. To identify new ligandable cysteines which can be hotspots therapy and better study cysteine oxidations, we develop cysteine-reactive probes, N -acryloylindole-alkynes (NAIAs), have superior reactivity owing delocalization π electrons acrylamide warhead over whole indole scaffold. This allows NAIAs probe functional more effectively...
A two-component platinum(II) complex–polymer ensemble has been demonstrated for label-free spectroscopic detection of glucose and α-glucosidase activity, based on the electrostatic assembly cationic complex molecules onto a glucose-bound anionic polymer.
An amphiphilic platinum(II)-containing diblock copolymer, with pH- and solvent-responsive micellization properties that could induce drastic UV-vis emission spectral changes via modulation of Pt⋯Pt π–π stacking interactions, has been demonstrated in both aqueous organic media.
The formation of polymer–metal complex aggregates and the FRET between PPE-SO3− several water-soluble cationic alkynylplatinum(II) complexes are revealed from UV-vis, steady-state emission time-resolved decay studies. From Stern–Volmer plots, [Pt{tpy(C6H4CH2NMe3-4)-4′}(CCC6H5)](OTf)2 (2) is found to be most efficient quencher at both low high concentrations. This has been ascribed its steric bulkiness stronger interactions with PPE-SO3−, hence largest association constant as well Förster...
Terpyridylplatinum(II)-based metallosupramolecular triblock copolymers with hydrophilic alkynyl ligands have been synthesised and characterised. As a result of the intrinsic properties Pluronics, reversible temperature-induced micellisation occurred at high temperature leading to aggregation platinum(II) complex moieties through Pt⋅⋅⋅Pt π-π interactions, resulting in significant UV/Vis absorption near-infrared (NIR) emission spectral changes. The critical micelle temperatures complexes were...
Two platinum(II) terpyridyl-based metallo-supramolecular triblock copolymers, [ClPt(tpy)PEO-PPO-PEO(tpy)PtCl](OTf)2 (1) and [(Ph–C≡C)Pt(tpy)PEO-PPO-PEO(tpy)Pt(C≡C–Ph)](OTf)2 (2), have been synthesized characterized. The two complexes were found to aggregate with Pt⋯Pt and/or π–π interactions at high temperature, which not reported so far, as revealed by UV/Vis absorption, emission 1H NMR study. This is due the formation of spherical micelles driven PEO-PPO-PEO copolymers temperatures above...
A series of water-soluble pH-responsive alkynylplatinum(II) terpyridine complexes have been synthesized and characterized. The electronic absorption, emission, electrochemical properties the studied. self-assembly processes representative in aqueous media, presumably through Pt···Pt and/or π–π interactions, investigated by concentration- temperature-dependent UV–vis absorption measurements dynamic light scattering experiments. Interestingly, some found to undergo induced disassembly media...
The ensemble of platinum(<sc>ii</sc>) complex and<italic>m</italic>PPE-Ala has been demonstrated for label-free detection G-quadruplex DNAs and the probing their topological transitions.
Water-soluble cationic alkynylplatinum(II) 2,6-bis(benzimidazol-2'-yl)pyridine (bzimpy) complexes have been demonstrated to undergo supramolecular assembly with anionic polyelectrolytes in aqueous buffer solution. Metal-metal-to-ligand charge transfer (MMLCT) absorptions and triplet MMLCT ((3) MMLCT) emissions found UV/Vis absorption emission spectra of the electrostatic non-conjugated polyelectrolytes, driven by Pt⋅⋅⋅Pt π-π interactions among complex molecules. Interestingly, two-component...