A5071543991- Cancer, Hypoxia, and Metabolism
- Protein Kinase Regulation and GTPase Signaling
- Cancer-related Molecular Pathways
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- Ubiquitin and proteasome pathways
- Cellular Mechanics and Interactions
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related gene regulation
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Cell Adhesion Molecules Research
- Cancer Mechanisms and Therapy
- Hepatocellular Carcinoma Treatment and Prognosis
- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
- Cancer, Lipids, and Metabolism
- FOXO transcription factor regulation
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Wnt/β-catenin signaling in development and cancer
- Marine animal studies overview
- Nuclear Structure and Function
- Cancer Immunotherapy and Biomarkers
University of Hong Kong
2015-2024
Chinese University of Hong Kong
2004-2024
Queen Mary Hospital
2013-2021
State Key Laboratory of Synthetic Chemistry
2016
Prince of Wales Hospital
2004-2011
CSY China-International Hepatitis Research Foundation
2010
Hong Kong Jockey Club
2008
University of Illinois Urbana-Champaign
2007
The University of Texas Medical Branch at Galveston
2004
Abstract Interaction between tumor cells and immune in the microenvironment is important cancer development. Immune interact with to shape this process. Here, we use single-cell RNA sequencing analysis delineate landscape heterogeneity a cohort of patients HBV-associated human hepatocellular carcinoma (HCC). We found that tumor-associated macrophages suppress T cell infiltration TIGIT-NECTIN2 interaction regulates immunosuppressive environment. The state transition towards more exhaustive...
Objective We investigated the effect and mechanism of hypoxic microenvironment hypoxia-inducible factors (HIFs) on hepatocellular carcinoma (HCC) cancer stemness. Design HCC stemness was analysed by self-renewal ability, chemoresistance, expression stemness-related genes stem cell (CSC) marker-positive population. Specific small ubiquitin-like modifier (SUMO) proteases 1 (SENP1) mRNA level examined with quantitative PCR in human paired HCCs. Immunoprecipitation used to examine binding...
MicroRNAs (miRNAs) regulate mRNA stability and protein expression, certain miRNAs have been demonstrated to act either as oncogenes or tumor suppressors. Differential miRNA expression signatures documented in many human cancers but the role of endometrioid endometrial cancer (EEC) remains poorly understood. This study identifies significantly dysregulated EEC cells, characterizes their impact on malignant phenotype. We studied 365 using Taqman low density arrays EECs normal endometriums....
The pathogenesis of hepatocellular carcinoma (HCC) is a multistep process involving the progressive accumulation molecular alterations pinpointing different and cellular events. next-generation sequencing technology facilitating global systematic evaluation landscapes in HCC. There emerging evidence supporting importance cancer metabolism tumor microenvironment providing favorable supportive niche to expedite HCC development. Moreover, recent studies have identified distinct surface markers...
Cancer metastasis is a multistep process that involves series of tumor‐stromal interaction, including extracellular matrix (ECM) remodeling, which requires concerted action multiple proteolytic enzymes and their endogenous inhibitors. This study investigated the role tissue inhibitor metalloproteinases (TIMP) 2 in context hepatocellular carcinoma (HCC) metastasis. We found TIMP2 was most significantly down‐regulated member among TIMP family human HCCs. Moreover, underexpression frequent...
<h3>Objective</h3> We investigated the mutational landscape of mammalian target rapamycin (mTOR) signalling cascade in hepatocellular carcinomas (HCCs) with chronic HBV background, aiming to evaluate and delineate mutation-dependent mechanism mTOR hyperactivation hepatocarcinogenesis. <h3>Design</h3> performed next-generation sequencing on human HCC samples cell line panel. Systematic screening pathway-related genes was undertaken mutant were evaluated based their recurrence. Protein...
Background and Aims Hepatitis B virus (HBV) integrations are common in hepatocellular carcinoma (HCC). In particular, alterations of the telomerase reverse transcriptase ( TERT ) gene by HBV frequent; however, molecular mechanism functional consequence underlying integration unclear. Approach Results We adopted a targeted sequencing strategy to survey human HBV‐associated HCCs (n = 95). at promoter was frequent (35.8%, n 34/95) HCC tumors associated with increased mRNA expression more...
Abstract Caveolin‐1 (Cav1) has been implicated in diverse human cancers, yet its role hepatocellular carcinoma (HCC) tumourigenesis and metastasis remains elusive. In the current study, we aim to provide a comprehensive understanding regarding functional of Cav1 HCC metastasis. expression was examined panel cell lines using western blotting analysis quantitative RT‐PCR tissues by immunohistochemistry. not detected normal liver line all non‐tumourous but exclusively expressed tissues....
Controversy over the benefits of antioxidants supplements in cancers persists for long. Using hepatocellular carcinoma (HCC) as a model, we investigated effects exogenous N-acetylcysteine (NAC) and glutathione (GSH) on tumor formation growth.Multiple mouse models, including diethylnitrosamine (DEN)-induced Trp53KO/C-MycOE-induced HCC hepatoma cell human xenograft models with subcutaneous or orthotopic injection were used. In vitro assays ROS assay, colony formation, sphere proliferation,...
Aims Deleted in liver cancer 1 (DLC1), a member of RhoGTPase activating protein (GAP) family, is known to have suppressive activities tumorigenicity and metastasis. However, the underlying molecular mechanisms how DLC1 suppresses cell motility not been fully elucidated. Rho-kinase (ROCK) an immediate down-stream effector RhoA mediating cellular cytoskeletal events motility. In present study, we aimed investigate effects on Rho/ROCK signaling pathway hepatocellular carcinoma (HCC)....
Hepatitis B virus (HBV) integration is common in HBV-associated hepatocellular carcinoma (HCC) and may play an important pathogenic role through the production of chimeric HBV-human transcripts. We aimed to screen transcriptome for HBV integrations HCCs.
Deleted in liver cancer 1 (DLC1) is a Rho GTPase-activating protein (RhoGAP) frequently deleted and underexpressed hepatocellular carcinoma (HCC) as well other cancers. Recent independent studies have shown interaction of DLC1 with members the tensin focal adhesion family Src Homology 2 (SH2) domain-dependent mechanism. tensins interact co-localize to punctate structures at adhesions. However, mechanisms underlying between various remain controversial.We used co-immunoprecipitation assay...
The focal adhesion protein Tensin4, also known as cten (c-terminal tensin like), is structurally distinct from the three other members in Tensin family. Its expression and potential functions cancers including hepatocellular carcinoma (HCC) are not well understood. With immunohistochemistry, 43% (13/30) of our human HCC cases showed up-regulation Tensin4 compared with their corresponding non-tumorous livers. In cells, treatment epidermal growth factor (EGF) significantly induced transcript...
// Wei Ma 1 , Karen Man-Fong Sze Lo Kong Chan Joyce Lee Larry Lai Chun-Ming Wong Terence Kin-Wah Carmen Chak-Lui Irene Oi-Lin Ng Department of Pathology and State Key Laboratory for Liver Research, The University Hong Kong, Pokfulam, Correspondence to: Ng, email: iolng@hku.hk Keywords: Rho GTPase, Rho-associated kinase, HCC, drug-resistance, cell survival Received: August 26, 2015 Accepted: April 18, 2016 Published: May ABSTRACT Small GTPase (Rho) its immediate effector kinase (ROCK) are...