A5012103892- Particle physics theoretical and experimental studies
- High-Energy Particle Collisions Research
- Single-cell and spatial transcriptomics
- Quantum Chromodynamics and Particle Interactions
- Cosmology and Gravitation Theories
- Dark Matter and Cosmic Phenomena
- Cancer Immunotherapy and Biomarkers
- Atherosclerosis and Cardiovascular Diseases
- Parallel Computing and Optimization Techniques
- Black Holes and Theoretical Physics
- Gene expression and cancer classification
- Physics of Superconductivity and Magnetism
- CAR-T cell therapy research
- Cutaneous lymphoproliferative disorders research
- Cell Image Analysis Techniques
- Cancer Cells and Metastasis
- Immune cells in cancer
- Gene Regulatory Network Analysis
- Immunotherapy and Immune Responses
- Matrix Theory and Algorithms
- Genetics, Aging, and Longevity in Model Organisms
- Molecular Biology Techniques and Applications
- Theoretical and Computational Physics
- Immune Response and Inflammation
- Cancer Genomics and Diagnostics
Broad Institute
2022-2025
Ludwig-Maximilians-Universität München
2022-2025
Massachusetts Institute of Technology
2022-2023
University of Regensburg
2014-2017
Forschungszentrum Jülich
2015-2017
Eötvös Loránd University
2015-2016
MTA-SZTE Research Group on Artificial Intelligence
2016
European Organization for Nuclear Research
2015
The spatial organization of cells and molecules plays a key role in tissue function homeostasis disease. Spatial transcriptomics has recently emerged as technique to capture positionally barcode RNAs directly tissues. Here, we advance the application at scale, by presenting Multi-Omics (SM-Omics) fully automated, high-throughput all-sequencing based platform for combined spatially resolved antibody-based protein measurements. SM-Omics uses DNA-barcoded antibodies, immunofluorescence or...
UNC93B1 is critical for trafficking and function of nucleic acid-sensing Toll-like receptors (TLRs) TLR3, TLR7, TLR8, TLR9, which are essential antiviral immunity. Overactive TLR7 signaling induced by recognition self-nucleic acids has been implicated in systemic lupus erythematosus (SLE). Here, we report variants (E92G R336L) four patients with early-onset SLE. Patient cells or mouse macrophages carrying the produced high amounts TNF-α IL-6 upon stimulation TLR7/TLR8 agonist, but not TLR3...
Axions are one of the most attractive dark matter candidates. The evolution their number density in early universe can be determined by calculating topological susceptibility $χ(T)$ QCD as a function temperature. Lattice provides an ab initio technique to carry out such calculation. A full result needs two ingredients: physical quark masses and controlled continuum extrapolation from non-vanishing zero lattice spacings. We determine quenched framework (infinitely large masses) extrapolate...
Abstract Transferring annotations of single-cell-, spatial- and multi-omics data is often challenging owing both to technical limitations, such as low spatial resolution or high dropout fraction, biological variations, continuous spectra cell states. Based on the concept that these are best described mixtures cells molecules, we present a computational framework for transfer their combinations (TACCO), which consists an optimal transport model extended with different wrappers annotate wide...
Most cell types in multicellular organisms can perform multiple functions. However, not all functions be optimally performed simultaneously by the same cells. Functions incompatible at level of individual cells population level, where divide labor and specialize different Division arise due to instruction tissue environment or through self-organization. Here, we develop a computational framework investigate contribution these mechanisms division within cell-type population. By optimizing...
Finite temperature charmonium spectral functions in the pseudoscalar and vector channels are studied lattice QCD with 2+1 flavours of dynamical Wilson quarks, on fine isotropic lattices (with a spacing 0.057fm), non-physical pion mass m π ≈ 545 MeV. The highest is approximately 1.4T c . Up to this no significant variation function seen channel. channel shows some dependence, which seems be consistent dependent low frequency peak related heavy quark transport, plus independent term at ω > 0....
Abstract Although metastatic disease is the leading cause of cancer-related deaths, its tumor microenvironment remains poorly characterized due to technical and biospecimen limitations. In this study, we assembled a multi-modal spatial cellular map 67 biopsies from 60 patients with breast cancer across diverse clinicopathological features nine anatomic sites detailed clinical annotations. We combined single-cell or single-nucleus RNA sequencing for all panel four expression assays...
While advances in single cell genomics have helped to chart the cellular components of tumor ecosystems, it has been more challenging characterize their specific spatial organization and functional interactions. Here, we combine RNA-seq, transcriptomics by Slide- seq, situ multiplex RNA analysis, create a detailed map healthy dysplastic colon ecosystems association with disease progression. We profiled inducible genetic CRC mouse models that recapitulate key features human CRC, assigned...
While advances in single cell genomics have helped to chart the cellular components of tumor ecosystems, it has been more challenging characterize their specific spatial organization and functional interactions. Here, we combine RNA-seq, transcriptomics by Slide- seq, situ multiplex RNA analysis, create a detailed map healthy dysplastic colon ecosystems association with disease progression. We profiled inducible genetic CRC mouse models that recapitulate key features human CRC, assigned...
We present a full result for the equation of state (EoS) in 2+1+1 (up/down, strange and charm quarks are present) flavour lattice QCD. extend this analysis give 2+1+1+1 In order to describe evolution universe from temperatures several hundreds GeV tens MeV we also include known effects electroweak theory effective degree freedoms. As another application QCD calculate topological susceptibility (chi) up few temperature region. These two results, EoS chi, can be used predict dark matter...
Abstract While advances in single cell genomics have helped to chart the cellular components of tumor ecosystems, it has been more challenging characterize their specific spatial organization and functional interactions. Here, we combine RNA-seq, transcriptomics by Slide- seq, situ multiplex RNA analysis, create a detailed map healthy dysplastic colon ecosystems association with disease progression. We profiled inducible genetic CRC mouse models that recapitulate key features human CRC,...
Understanding of the transport properties quark-gluon plasma is becoming increasingly important to describe current measurements at heavy ion collisions.This work reports on recent efforts determine shear viscosity η in deconfined phase from lattice QCD.The main focus integration Wilson flow analysis get a better handle infrared behaviour spectral function which relevant for transport.It carried out finite time, eliminates dependence spacing.Eventually, new continuum limit has be sends...
We use the Wilson flow to define gauge anisotropy at a given physical scale. demonstrate of anisotropic by performing tuning bare in tree-level Symanzik action for several lattice spacings and target anisotropies. this method tune parameters full QCD, where we also exploit diminishing effect well chosen smearing on renormalization fermion anisotropy.
A common problem in lattice QCD simulations on the torus is extremely long autocorrelation time of topological charge when one approaches continuum limit. The reason suppressed tunneling between sectors. can be circumvented by replacing with a different manifold, so that connectivity configuration space changed. This achieved using open boundary conditions fields, as proposed earlier. It has side effect breaking translational invariance strongly. Here we propose to use nonorientable manifold...
We give an overview of QPACE 2, which is a custom-designed supercomputer based on Intel Xeon Phi processors, developed in collaboration Regensburg University and Eurotech.We some general recommendations for how to write high-performance code the then discuss our implementation domain-decomposition-based solver present number benchmarks.
Abstract Rapid advances in single-cell-, spatial-, and multi-omics, allow us to profile cellular ecosystems tissues at unprecedented resolution, scale, depth. However, both technical limitations, such as low spatial resolution biological variations, continuous spectra of cell states, often render these data imperfect representations systems, best captured mixtures over cells or molecules. Based on this conceptual insight, we build a versatile framework, TACCO (Transfer Annotations Cells...
Finite temperature charmonium spectral functions in the pseudoscalar(PS) and vector(V) channels are studied lattice QCD with 2+1 flavours of dynamical Wilson quarks, on fine isotropic lattices (with a spacing 0.057fm), non-physical pion mass 545MeV. The highest is approximately 1.4Tc. Up to this no significant variation function seen PS channel. V channel shows some dependence, which seems be consistent dependent low frequency peak related heavy quark transport, plus independent term at...
Finite temperature charmonium spectral functions in the pseudoscalar(PS) and vector(V) channels are studied lattice QCD with 2+1 flavours of dynamical Wilson quarks, on fine isotropic lattices (with a spacing 0.057fm), non-physical pion mass 545MeV. The highest is approximately 1.4Tc. Up to this no significant variation function seen PS channel. V channel shows some dependence, which seems be consistent dependent low frequency peak related heavy quark transport, plus independent term at...
<h3>Background</h3> The majority cutaneous T cell lymphoma (CTCL) run an indolent clinical course. However, advanced stages (>=IIB) or certain CTCL subtypes such as folliculotropic Mycosis Fungoides (FMF) and Sézary Syndrome (SS) are associated with significantly worse prognosis treatment options limited. In previous studies, our group has described increased numbers of tissue infiltrating B cells adverse prognostic factor. the underlying mechanisms still poorly understood.This project...