The dynamic interface between the cellular membrane and 3D nanostructures determines biological processes guides design of novel biomedical devices. Despite fact that recent advancements in fabrication artificial biointerfaces have yielded an enhanced understanding this interface, there remain open questions on how reacts behaves presence sharp objects nanoscale. Here we provide a multifaceted characterization membrane's mechanical stability when closely interacting with high-aspect-ratio...

Delivery of molecules into intracellular compartments is one the fundamental requirements in molecular biology. However, possibility delivering a precise number nano-objects with single-particle resolution still an open challenge. Here we present electrophoretic platform based on 3D hollow nanoelectrodes to enable delivery single nanoparticles selected cells and monitoring by surface-enhanced Raman scattering (SERS). The gold-coated nanoelectrode capable confinement enhancement...

Electroporation of in-vitro cultured cells is widely used in biological and medical areas to deliver molecules interest inside cells. Since very high electric fields are required electroporate the plasma membrane, depending on geometry electrodes voltages can be often critical cell viability. Furthermore, traditional electroporation configuration based planar there no a priori certain feedback about which has been targeted delivered addition fluorophores may needed gain this information. In...

Graphene with its unique electrical properties is a promising candidate for carbon-based biosensors such as microelectrodes and field effect transistors. Recently, graphene were successfully used extracellular recording of action potentials in electrogenic cells; however, intracellular recordings remain beyond their current capabilities because the lack an efficient cell poration method. Here, we present microelectrode platform consisting out-of-plane grown three-dimensional fuzzy (3DFG)...

Abstract Presently, the in vitro recording of intracellular neuronal signals on microelectrode arrays (MEAs) requires complex 3D nanostructures or invasive and approaches such as electroporation. Here, it is shown that laser poration enables coupling planar electrodes without damaging neurons altering their spontaneous electrophysiological activity, allowing process to be repeated multiple times same cells. This capability distinguishes laser‐based neuron from more methods like...

A platform that enables simultaneous targeted drug delivery to single/few cells and intracellular recording at a network level from large cell populations.

Live intracellular imaging is a valuable tool in modern diagnostics and pharmacology. Surface Enhanced Raman Spectroscopy (SERS) stands out as non-destructive multiplexed technique, but SERS still suffers from interfering background endogenous components. Here we show the assembly of small colloidal probes with signal cell-silent window 1800-2900 cm-1 for biorthogonal dopamine that was undistinguishable cell background. By linking silver nanoparticles alkyne-dopamine adducts, clusters are...

Abstract 3D vertical nanostructures have become one of the most significant methods for interfacing cells and nanoscale accessing intracellular functionalities such as membrane potential. As this access can be induced by means diverse cellular poration mechanisms, it is important to investigate in detail cell condition after rupture assessing real effects techniques on biological environment. Indeed, differences dynamics reshaping not been observed yet when membrane‐nanostructure system...

High quality attenuated intracellular action potentials from large cell networks can be recorded on multi-electrode arrays by means of 3D vertical nanopillars using electrical pulses. However, most the techniques require complex nanostructures that prevent straightforward translation into marketable products and wide adoption in scientific community. Moreover, are often delicate objects cannot sustain several harsh use/cleaning cycles. On contrary, laser optoacoustic poration allows...

Neuropathological models and neurological disease progression treatments have always been of great interest in biomedical research because their impact on society. The application vitro microfluidic devices to neuroscience-related disciplines provided several advancements therapeutics or neuronal modeling thanks the ability control cellular microenvironment at spatiotemporal level. Recently, introduction three-dimensional nanostructures has allowed high performance both recording...

The use of gold nanoparticles for hyperthermia therapy in near infrared (NIR) spectral regions has catalysed substantial research efforts due to the potential impact clinical applications. However, photoscattering effect scaling with square nanoparticle volume leads a low absorption efficiency, which hindered utility NIR II above 1000 nm. Here, we conquer this limit by introducing hyperbolic metamaterial that are made multi-layered gold/dielectric nanodisks and exhibit >70% efficiency III...

Optical stimulation technologies are gaining great consideration in cardiology, neuroscience studies, and drug discovery pathways by providing control over cell activity with high spatio-temporal resolution. However, this precision requires manipulation of biological processes at genetic level concealing its development from broad scale application. Therefore, translating these into tools for medical or pharmacological applications remains a challenge. Here, an all-optical nongenetic method...

In vitro multi-electrode array (MEA) technology is nowadays involved in a wide range of applications beyond neuroscience, such as cardiac electrophysiology and bio-interface studies. However, the cost commercially available acquisition systems severely limits its adoption outside specialized laboratories with high budget capabilities. Thus, availability low-cost methods to acquire signals from MEAs important allow research labs worldwide exploit this for an ever-expanding pool experiments...

The electrophysiological recording of action potentials in human cells is a long-sought objective due to its pivotal importance many disciplines. Among the developed techniques, invasiveness remains common issue, causing cytotoxicity or altering unpredictably cell physiological response. In this work, new approach for intracellular signals outstanding quality and with noninvasiveness introduced. By taking profit concept mirror charge classical electrodynamics, proposed device transduces...

Abstract The reliable identification of chronic cardiotoxic effects in vitro screenings is fundamental for filtering out toxic molecular entities before vivo animal experimentation and clinical trials. Present techniques such as patch-clamp, voltage indicators, standard microelectrode arrays do not offer at the same time high sensitivity measuring transmembrane ion currents low-invasiveness monitoring cells over long time. Here, we show that optoporation applied to enables action potentials...

Drug-induced cardiotoxicity is a major barrier to drug development and main cause of withdrawal marketed drugs. Drugs can strongly alter the spontaneous functioning heart by interacting with cardiac membrane ion channels. If these effects only surface during in vivo preclinical tests, clinical trials or worse after commercialization, societal economic burden will be significant seriously hinder efficient process. Hence, safety pharmacology requires vitro electrophysiological screening assays...

In vivo cell niches are complex architectures that provide a wide range of biochemical and mechanical stimuli to control behavior fate. With the aim in vitro microenvironments mimicking physiological niches, microstructured substrates have been exploited support adhesion shape as well three dimensional morphology. At variance with previous methods, we propose simple rapid protein subtractive soft lithographic method obtain polydimethylsiloxane for studying stem growth. The adult renal cells...

In this work we present a device able to electroporate and deliver molecules inside adherent cells using hollow nanoelectrodes that act also as nanofluidic channels. Our fabrication approach allows greatly reduce the amplitude of applied electrical pulse train selectively only targeted cells.

Biological studies on in vitro cell cultures are of fundamental importance to investigate cells response external stimuli, such as new drugs for treatment specific pathologies, or study communication between electrogenic cells. Although three-dimensional (3D) nanostructures brought tremendous improvements biosensors used various biological studies, including drug delivery and electrical recording, there is still a lack multifunctional capabilities that could help gaining deeper insights...

The use of gold nanoparticles for hyperthermia therapy in near infrared (NIR) spectral regions has catalysed substantial research efforts due to the potential impact clinical applications. However, photoscattering effect scaling with square nanoparticle volume leads a low absorption efficiency, which hindered utility NIR II above 1000 nm. Here, we conquer this limit by introducing hyperbolic metamaterial that are made multi-layered gold/dielectric nanodisks and exhibit >70% efficiency III...