A5047189927- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Gold and Silver Nanoparticles Synthesis and Applications
- Silicon Nanostructures and Photoluminescence
- Nanowire Synthesis and Applications
- Cellular Mechanics and Interactions
- Plasmonic and Surface Plasmon Research
- Photoreceptor and optogenetics research
- Advanced Memory and Neural Computing
- Lipid Membrane Structure and Behavior
- Force Microscopy Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Anodic Oxide Films and Nanostructures
- Spectroscopy Techniques in Biomedical and Chemical Research
- Pluripotent Stem Cells Research
- Nanoplatforms for cancer theranostics
- Quantum Dots Synthesis And Properties
- Congenital heart defects research
- Mass Spectrometry Techniques and Applications
- Metamaterials and Metasurfaces Applications
- Optical Coatings and Gratings
- Microbial Inactivation Methods
- Virus-based gene therapy research
- bioluminescence and chemiluminescence research
King's College London
2020-2024
London Centre for Nanotechnology
2022-2024
Italian Institute of Technology
2015-2020
Transnational Press London
2020
University of Genoa
2016-2019
Istituto Nazionale di Fisica Nucleare, Sezione di Genova
2018
Sapienza University of Rome
2014
Three-dimensional vertical micro- and nanostructures can enhance the signal quality of multielectrode arrays promise to become prime methodology for investigation large networks electrogenic cells. So far, access intracellular environment has been obtained via spontaneous poration, electroporation, or by surface functionalization micro/nanostructures; however, these methods still suffer from some limitations due their intrinsic characteristics that limit widespread use. Here, we demonstrate...
A Universal plasmonic/microfluidic platform for spatial and temporal controlled intracellular delivery is described. The system can inject/transfect the desired amount of molecules with an efficacy close to 100%. Moreover, it highly scalable from single cells large ensembles without administering extracellular bath. latter enables quantitative control over injected molecules. We present in vitro delivering a broad range into compartment cell selective high parallelization potential. achieved...
Abstract Periodontal disease is a significant burden for oral health, causing progressive and irreversible damage to the support structure of tooth. This complex structure, periodontium, composed interconnected soft mineralised tissues, posing challenge regenerative approaches. Materials combining silicon lithium are widely studied in periodontal regeneration, as they stimulate bone repair via silicic acid release while providing stimuli through activation Wnt/β-catenin pathway. Yet,...
The dynamic interface between the cellular membrane and 3D nanostructures determines biological processes guides design of novel biomedical devices. Despite fact that recent advancements in fabrication artificial biointerfaces have yielded an enhanced understanding this interface, there remain open questions on how reacts behaves presence sharp objects nanoscale. Here we provide a multifaceted characterization membrane's mechanical stability when closely interacting with high-aspect-ratio...
Delivery of molecules into intracellular compartments is one the fundamental requirements in molecular biology. However, possibility delivering a precise number nano-objects with single-particle resolution still an open challenge. Here we present electrophoretic platform based on 3D hollow nanoelectrodes to enable delivery single nanoparticles selected cells and monitoring by surface-enhanced Raman scattering (SERS). The gold-coated nanoelectrode capable confinement enhancement...
Electroporation of in-vitro cultured cells is widely used in biological and medical areas to deliver molecules interest inside cells. Since very high electric fields are required electroporate the plasma membrane, depending on geometry electrodes voltages can be often critical cell viability. Furthermore, traditional electroporation configuration based planar there no a priori certain feedback about which has been targeted delivered addition fluorophores may needed gain this information. In...
Gaining access to the cell interior is fundamental for many applications, such as electrical recording and drug biomolecular delivery. A very promising technique consists of culturing cells on micro-/nanopillars. The tight adhesion high local deformation in contact with nanostructures can promote permeabilization lipids at plasma membrane, providing internal compartment. However, there still much experimental controversy regarding when how intracellular environment targeted role geometry...
Abstract 3D nanostructures are widely exploited in cell cultures for many purposes such as controlled drug delivery, transfection, intracellular sampling, and electrical recording. However, little is known about the interaction of cells with these substrates, even less effects electroporation on cellular membrane nuclear envelope. This work exploits plasmonic nanoelectrodes to study, by surface‐enhanced Raman scattering (SERS), dynamics nanostructured substrate before, during, after...
A platform that enables simultaneous targeted drug delivery to single/few cells and intracellular recording at a network level from large cell populations.
Abstract Multicellular tumour cell spheroids embedded within three‐dimensional (3D) hydrogels or extracellular matrices (ECM) are widely used as models to study cancer growth and invasion. Standard methods embed in 3D result random placement space which limits the use of inverted fluorescence microscopy techniques, thus resolution that can be achieved image molecular detail intact spheroid. Here, we leverage UV photolithography microfabricate PDMS (polydimethylsiloxane) stamps allow for...
Live intracellular imaging is a valuable tool in modern diagnostics and pharmacology. Surface Enhanced Raman Spectroscopy (SERS) stands out as non-destructive multiplexed technique, but SERS still suffers from interfering background endogenous components. Here we show the assembly of small colloidal probes with signal cell-silent window 1800-2900 cm-1 for biorthogonal dopamine that was undistinguishable cell background. By linking silver nanoparticles alkyne-dopamine adducts, clusters are...
Abstract 3D vertical nanostructures have become one of the most significant methods for interfacing cells and nanoscale accessing intracellular functionalities such as membrane potential. As this access can be induced by means diverse cellular poration mechanisms, it is important to investigate in detail cell condition after rupture assessing real effects techniques on biological environment. Indeed, differences dynamics reshaping not been observed yet when membrane‐nanostructure system...
Abstract: In order to deliver low-cost viral capsomeres from a large amount of soluble VP6 protein human rotavirus, we developed and optimized biotechnological platform in Escherichia coli . Specifically, three different expression protocols were compared, differing their genetic constructs, ie, simple native histidine-tagged sequence, fused thioredoxin, obtained with the newly described small ubiquitin-like modifier (SUMO) fusion system. Our results demonstrate that does not escape...
Abstract Engineering the spatial organisation of organotypic cultures is pivotal for refining tissue models that are useful gaining deeper insights into complex, non‐cell autonomous processes. These advanced key to improving understanding fundamental biological mechanisms and therapeutic strategies. Controlling gene regulation through spatially‐resolved delivery nucleic acids provides an attractive approach produce such models. An emerging strategy transfection uses photosensitizing...
Abstract Mapping the molecular composition of tissues using spatial biology provides high-content information for diagnostics. However, approaches require invasive procedures to collect samples and destroy investigated tissue, limiting extent analysis, particularly highly functional such as those brain. To address these limitations, we developed a workflow harvest biomolecules from brain nanoneedles characterise distribution lipids desorption electrospray ionization mass spectrometry...
The epicardium has recently gained interest in the cardiovascular field due to its capacity support heart regeneration after ischemic injury. Models study of large animals vitro are limited and mainly based on epicardial cell isolation/differentiation from stem cells, followed by 2D cells culture. In this method paper, we describe procedure obtain culture 3D organotypic slices presenting an intact epicardium, as a novel model physiology activation. Epicardial obtained porcine hearts using...
We developed a platform based on 3D plasmonic nanoantennas able to perform different functions with applications in the biological research area. In particular it will be shown how peculiar geometry of system plays fundamental role, leading tight interaction cellular membrane. Such configuration allows one side investigation extracellular features through enhanced vibrational spectroscopy and electrical recording, other possibility intracellular injection by optoporation. this regard...
Gaining access to the cell interior is fundamental for many applications, such as electrical recording, drug and biomolecular delivery. A very promising technique consists of culturing cells on nano/micro pillars. The tight adhesion high local deformation in contact with nanostructures can promote permeabilization lipids at plasma membrane, providing internal compartment. However, there still much experimental controversy regarding when how intracellular environment targeted role geometry...