Abstract Background MicroRNAs (miRNAs), a ubiquitous class of short RNAs, play vital roles in physiological and biochemical processes plants by mediating gene silencing at post-transcriptional (PTGS) level. Tomato is model system to study molecular basis fleshy fruit ripening senescence, ethylene biosynthesis signal transduction owing its genetic tractability. To the functions miRNAs tomato their possible response, next generation sequencing method was employed identify fruit. Bioinformatics...

ABSTRACT The nitrate and nitrite reductases of Bacillus subtilis have two different physiological functions. Under conditions nitrogen limitation, these enzymes catalyze the reduction via to ammonia for anabolic incorporation into biomolecules. They also function catabolically in anaerobic respiration, which involves use as terminal electron acceptors. Two distinct reductases, encoded by narGHI nasBC , catabolic metabolism, respectively. However, reported herein, a single NADH-dependent,...

Abstract Eggplant ( Solanum melongena ) is an economically important vegetable requiring investigation into its various genomic functions. The current limitation in the of function eggplant lack effective tools available for conducting functional assays. Virus‐induced gene silencing (VIGS) has played a critical role genetic analyses. In this paper, TRV‐mediated VIGS was successfully elicited eggplant. We first cloned CDS sequence PDS PHYTOENE DESATURASE and then silenced gene....

Mutations in clpP and clpX have pleiotropic effects on growth developmentally regulated gene expression Bacillus subtilis . ClpP ClpX are needed for of comK , encoding the competence transcription factor required genes within regulon. ClpP, combination with ATPase ClpC, degrades inhibitor ComK, MecA. Proteolysis MecA is stimulated by a small protein, ComS, which interacts Suppressor mutations ( cxs ) were isolated that bypass requirement expression. These found also to overcome defect...

The mouse sucrase-isomaltase (SI) gene is an enterocyte-specific expressed in a complex developmental pattern. We previously reported that short, evolutionarily conserved promoter regulates expression of SI small intestine. Herein, we investigated the role hepatocyte nuclear factor-1 (HNF-1) cis-acting element to regulate vivo. Transgenic constructs with mutated HNF-1 (SIF3) revealed strong reduction activity comparison wild-type construct mice and during Caco-2 cell differentiation. Nuclear...

Abstract Background Sufentanil-induced cough is common during the induction of anesthesia. The objective this study was to determine whether pretreatment with a small dose esketamine effective in treating sufentanil-induced cough. Methods 220 patients were screened, and 200 who had scheduled elective surgery between 18 70 years old randomly divided into two groups. Before sufentanil administered, group (group K) injected 0.15 mg/kg at 5 s, control C) administered same volume. Within 1 min...

The effects of sulfur fertilization on the growth profile, contents glucosinolates, anthocyanins, vitamin C, carotenoids, chlorophylls, total phenolics, and FRAP value in radish seeds sprouts were investigated. concentrations glucosinolates antioxidants strongly influenced by process germination. Sulfur induced significant increases individual phenolics. phenolic cultivated using 20, 60, or 100 mg/L sulfate 20.7%, 40.4%, 40.8% higher, respectively, than those 7-day-old control sprouts. No...

The two‐component regulatory proteins ResD and ResE are required for anaerobic nitrate respiration in Bacillus subtilis . ResD, when it undergoes ResE‐dependent phosphorylation, is thought to activate transcriptionally anaerobically induced genes such as fnr , hmp nasD In this report, deletion analysis of the promoter regions was carried out identify cis ‐acting sequences ResDE‐dependent transcription. results suggest that promoters have multiple target regulation has a single site. Gel...

The Bacillus subtilis clpX and clpP genes are the sites of pleiotropic mutations that adversely affect growth on a variety media impair developmental processes such as sporulation competence development. ClpX is necessary for post‐exponential induction require σ H form RNA polymerase transcription. Both ClpP required activation A ‐dependent transcription srf operon encodes surfactin synthetase regulatory peptide ComS, development genetic competence. Transcription activated by two‐component...

Summary. Interferon alpha (IFN‐α) therapy is widely used to treat patients with chronic hepatitis B (CHB) but the sustained response rate low, and molecular mechanisms for ineffectiveness of IFN‐α treatments are not known. We screened differentially expressed genes between responders (Rs) nonresponders (NRs) in CHB treated explore basis treatment failure. Expression profiling was performed on percutaneous needle liver biopsy specimens taken before therapy. Gene expression levels were...

The ResD-ResE signal transduction system is required for transcription of genes involved in aerobic and anaerobic respiration Bacillus subtilis. Phosphorylated ResD (ResD approximately P) interacts with target DNA to activate transcription. A strong sequence similarity was detected promoter regions some ResD-controlled including fnr resA. Single-base substitutions the resA promoters were performed determine a ResD-binding sequence. DNase I footprinting analysis indicated that P itself does...

The Bacillus subtilis ResD-ResE two-component regulatory system activates genes involved in nitrate respiration response to oxygen limitation or nitric oxide (NO). sensor kinase ResE the regulator ResD through phosphorylation, which then binds region of anaerobiosis activate their transcription. is composed an N-terminal signal input domain and a C-terminal catalytic domain. contains two transmembrane subdomains large extracytoplasmic loop. It also has cytoplasmic PAS subdomain between HAMP...

BACKGROUND: LE-RIN encodes the RIN (Ripening Inhibitor) transcription factor belonging to MADS (Mcm1, Agamous, Deficiens, Srf) box family and regulates tomato ripening. The plays an indispensable role in ethylene biosynthesis. In study, we respectively evaluated gene expressions enzymatic activities of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) ACC oxidase (ACO) production understand transcriptional regulation RESULTS: generates strong or weak induction expression LE-ACS2,...

ABSTRACT The ResD-ResE signal transduction system is required for aerobic and anaerobic respiration in Bacillus subtilis . histidine sensor kinase ResE, by functioning as a phosphatase the cognate response regulator ResD, controls level of phosphorylated ResD. A high ResD postulated to cause dramatic increase transcription ResDE-controlled genes under conditions. mutant which retains autophosphorylation phosphorylation activities but defective dephosphorylation, allowed partially derepressed...

Small RNAs have emerged as critical regulators in the expression and function of eukaryotic genomes at post‐transcriptional level. To elucidate functions microRNA ( miRNAs ) endogenous small‐interfering siRNAs tomato fruit ripening process, deep sequencing bioinformatics methods were combined to parse small landscape three fruit‐ripening stages (mature green, breaker red‐ripe) on a whole genome. Two species‐specific two members TAS3 family identified, 590 putative phased 125 cis ‐natural...

Abstract Aims Veno‐arterial extracorporeal membrane oxygenation (VA‐ECMO) is an important technique for the treatment of refractory cardiogenic shock and cardiac arrest; however, early management ventricular fibrillation/ventricular tachycardia (VF/VT), within 72 h VA‐ECMO, its effects on patient prognosis remain unclear. Methods results We retrospectively analysed patients at First Affiliated Hospital Nanjing Medical University who underwent VA‐ECMO between January 2017 March 2022. The were...

ABSTRACT The Bacillus subtilis ResD-ResE two-component signal transduction system is essential for aerobic and anaerobic respiration. A spontaneous suppressor mutant that expresses ResD-controlled genes grows anaerobically in the absence of ResE histidine kinase was isolated. In addition, expression suppressed strain constitutive occurred at a much higher level than observed wild-type strain. suppressing mutation, which mapped to pgk , gene encoding 3-phosphoglycerate kinase, failed suppress...

Background Although periodic blood shortages are widespread in major Chinese cities, approximately 1x105 U of whole discarded yearly because under-collection. To reduce the wastage acid citrate dextrose solution B (ACD-B) anticoagulated under-collected (UC-WB), this study was performed to elucidate effect extracellular pH and holding time on erythrocyte quality. Mannitol-adenine-phosphate (MAP) concentrates (UC-RBCs) were prepared with UC-WB assess safety efficacy component. Methods The...

<title>Abstract</title> Background The success rate of venoarterial extracorporeal membrane oxygenation (VA-ECMO) is related to complications such as nosocomial infection (NI), with multidrug-resistant organisms (MDROs) posing the toughest challenge, however, incidence, risk factors, and prognosis NI during VA-ECMO are unclear. Methods We performed a single-center, retrospective analysis 152 patients receiving VA-ECMO. Patients were categorized into non-NI groups, depending on whether...

<title>Abstract</title> Study objective: Despite being one of the most common adverse effects following thyroidectomy, there are currently no known effective preventive or therapeutic measures for postoperative nausea and vomiting (PONV). The purpose this prospective randomised controlled trial is to assess if any correlation between in patients having thyroidectomy 20° Trendelenburg posture during surgical preparation. Methods 180 undergoing elective who were 18 65 years old randomly...