A5009119759- Cardiovascular Function and Risk Factors
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Cardiovascular Disease and Adiposity
- Metabolomics and Mass Spectrometry Studies
- Protein Tyrosine Phosphatases
- Chemotherapy-induced cardiotoxicity and mitigation
- Metabolism, Diabetes, and Cancer
- Metabolism and Genetic Disorders
- Cardiac Ischemia and Reperfusion
- Diet, Metabolism, and Disease
- Liver Disease Diagnosis and Treatment
- Cardiac electrophysiology and arrhythmias
- Cardiac Valve Diseases and Treatments
- Cancer, Hypoxia, and Metabolism
- Nitric Oxide and Endothelin Effects
- Advanced MRI Techniques and Applications
- Cardiomyopathy and Myosin Studies
- Antioxidant Activity and Oxidative Stress
- Electron Spin Resonance Studies
- Cardiovascular Effects of Exercise
- Sirtuins and Resveratrol in Medicine
- Cardiac Imaging and Diagnostics
- Redox biology and oxidative stress
Case Western Reserve University
2009-2023
Indiana University School of Medicine
2019
Indiana University – Purdue University Indianapolis
2019
Institute for Musculoskeletal Health
2019
University School
2016-2017
University of Minnesota
2005-2007
University of Minnesota, Duluth
2001-2007
Cardiomyopathy is a major complication of diabetes. Our study was aimed to identify the sites mitochondrial dysfunction and delineate its consequences on metabolism in model type 1 Diabetes induced by streptozotocin injection male Lewis rats. We found decrease biogenesis pathway electron transport chain complex assembly that targets Complex I. Oxidation II long-chain fatty acid substrates support leak superoxide production. Mitochondrial defects do not limit oxidation as heart's preferred...
Biological systems that produce or are exposed to nitric oxide (NO radical) exhibit changes in the rate of oxygen free radical production. Considering mitochondria main intracellular source radicals, and based on recently documented production NO(radical) by intact mitochondria, we investigated whether NO(radical), produced mitochondrial nitric-oxide synthase, could affect generation radicals. Toward this end, H(2)O(2) rat liver were monitored at different rates endogenous The observed...
A metabolomic approach to selectively profile all acyl-CoAs was developed using a programmed multiple reaction monitoring (MRM) method in LC-MS/MS and employed the analysis of various rat organs. The MRM possessed 300 mass ion transitions with difference 507 between precursor (Q1) product (Q3), started from m/z 768 progressively increased one unit at each step. Acyl-dephospho-CoAs resulting dephosphorylation were identified by accurate MS fragmentation. also quantitatively scanned 427 Q1 Q3...
Regulation of blood pH-critical for virtually every facet life-requires that the renal proximal tubule (PT) adjust its rate H(+) secretion (nearly same as HCO3 (-) reabsorption, JHCO3 ) in response to changes [CO2] and [HCO3 (-)]. Yet CO2/HCO3 sensing mechanisms remain poorly characterized. Because receptor tyrosine kinase inhibitors render PT insensitive CO2 concentration, we hypothesized structural features protein phosphatase-γ (RPTPγ) are consistent with binding extracellular or...
Previous studies have reported that high fat feeding in mild to moderate heart failure (HF) results the preservation of contractile function. Recent evidence has suggested preventing switch from fatty acid glucose metabolism HF may ameliorate dysfunction, and insulin resistance is one potential mechanism for regulating substrate utilization. This study was designed determine whether peripheral myocardial exists with and/or a high-fat diet signaling altered accordingly. Rats underwent...
Dietary fructose causes salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the filtered NaCl. Angiotensin II (Ang II), atrial natriuretic peptide (ANP) and norepinephrine (NE) regulate this process. Although Ang signaling blockade ameliorates fructose-induced hypertension, basal PT Na+ reabsorption its sensitivity to aforementioned factors have not been studied in model. We hypothesized consuming with a high-salt diet selectively enhances transport II. investigated effects...
Impaired myocardial contractile function is a hallmark of heart failure (HF), which may present under resting conditions and/or during physiological stress. Previous studies have reported that high fat feeding in mild to moderate HF/left ventricular (LV) dysfunction associated with improved at baseline. The goal this study was determine whether compromised response stress and evaluate the global gene expression profile rats fed dietary after infarction. Male Wistar underwent ligation or sham...
Thyroid hormone (TH) is recognized for its role in cellular metabolism and growth participates homeostasis of the heart. T3 activates pro-survival pathways including Akt mTOR. Treatment with after myocardial infarction cardioprotective promotes elements physiological hypertrophic response cardiac injury. Although known to benefit heart, very little about regulation at molecular level has been described date. The ubiquitin proteasome system (UPS) regulates nuclear receptors such as estrogen,...
Diabetic cardiomyopathy is preceded by mitochondrial alterations, and progresses to heart failure. We studied whether treatment with methylene blue (MB), a compound that was reported serve as an alternate electron carrier within the transport chain (ETC), improves metabolism cardiac function in type 1 diabetes. MB administered at 10 mg/kg/day control diabetic rats. Both echocardiography hemodynamic studies were performed assess function. Mitochondrial comprised measurement of oxidative...
Fructose-enriched diets cause salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the water and salt filtered through glomerulus. Angiotensin II (Ang II) regulates this process. Normally, dietary reduces Ang allowing kidney to excrete more salt, thereby preventing We hypothesized that fructose-enriched enhance ability low concentrations stimulate PT transport. measured effects a concentration (10-12 mol/L) on transport-related oxygen consumption (QO₂), Na/K-ATPase...
Pathological conditions such as diabetes, insulin resistance, and obesity are characterized by elevated plasma myocardial lipid levels have been reported to exacerbate the progression of heart failure (HF). Alterations in cardiomyocyte Ca(2+) regulatory properties myofilament proteins also implicated contractile dysfunction HF. However, our prior studies that high saturated fat (SAT) feeding improves vivo function, thereby exerting a cardioprotective effect Therefore, we hypothesized SAT...