A5100429502- 3D Printing in Biomedical Research
- Biosensors and Analytical Detection
- Microfluidic and Bio-sensing Technologies
- Cellular Mechanics and Interactions
- Semiconductor materials and devices
- Advanced biosensing and bioanalysis techniques
- Nanowire Synthesis and Applications
- Semiconductor materials and interfaces
- Single-cell and spatial transcriptomics
- Hepatitis B Virus Studies
- Acoustic Wave Resonator Technologies
- Chemical Looping and Thermochemical Processes
- Neuroscience and Neural Engineering
- Advanced Surface Polishing Techniques
- Advanced Biosensing Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Hydrogels: synthesis, properties, applications
- Tissue Engineering and Regenerative Medicine
- Artificial Intelligence in Healthcare and Education
- COVID-19 diagnosis using AI
- Liver physiology and pathology
- Advanced Sensor and Energy Harvesting Materials
- Clinical Reasoning and Diagnostic Skills
- Fuel Cells and Related Materials
Duke University
2023-2024
Creative Commons
2023
Discovery Centre
2022
McMaster University
2022
Hannam University
2022
Queen's University
2022
University of British Columbia
2022
Stanford University
2008-2021
Stanford Medicine
2021
Pohang University of Science and Technology
2014-2015
The vast majority of microfluidic devices are developed in PDMS by molding ("soft lithography") because is an inexpensive material, has physicochemical properties that well suited for biomedical and physical sciences applications, design cycle lengths generally adequate prototype development. However, tediously slow thus cannot provide the high- or medium-volume production required commercialization devices. While high-throughput plastic techniques (e.g. injection molding) exist, exorbitant...
A facile method has been developed to detect pathogenic bacteria using magnetic nanoparticle clusters (MNCs) and a 3D-printed helical microchannel. Antibody-functionalized MNCs were used capture E. coli (EC) in milk, the free MNC-EC complexes separated from milk permanent magnet. The dispersed buffer solution, then solution was injected into microchannel device with or without sheath flow. via Dean drag force lift force, separation facilitated presence of concentration determined light...
We developed a novel 3D immunomagnetic flow assay for the rapid detection of pathogenic bacteria in large-volume food sample. Antibody-functionalized magnetic nanoparticle clusters (AbMNCs) were magnetically immobilized on surfaces 3D-printed cylindrical microchannel. The injection Salmonella-spiked sample solution into microchannel produced instant binding between AbMNCs and Salmonella due to their efficient collisions. Nearly perfect capture AbMNCs-Salmonella complexes was achieved under...
Emerging artificial intelligence (AI) technologies have diverse applications in medicine. As AI tools advance towards clinical implementation, skills how to use and interpret a healthcare setting could become integral for physicians. This study examines undergraduate medical students' perceptions of AI, educational opportunities about medicine, the desired medium curriculum delivery.A 32 question survey students was distributed from May-October 2021 all 17 Canadian schools. The assessed...
Cell-cell interactions influence all aspects of development, homeostasis, and disease. In cancer, between cancer cells stromal play a major role in nearly every step carcinogenesis. Thus, the ability to record cell-cell would facilitate mechanistic delineation microenvironment. Here, we describe GFP-based Touching Nexus (G-baToN) which relies upon nanobody-directed fluorescent protein transfer enable sensitive specific labeling after interactions. G-baToN is generalizable system that enables...
In this study, we investigated whether hepatitis B virus (HBV) causes the alteration of lipid metabolism and composition during acute infection liver regeneration in a mouse model. The controls biogenesis bile acid homeostasis. Infection HBV various diseases impairs regeneration. As there are very few reports available literature on alterations by or HBV-mediated injury, have analyzed phospholipids that important roles using matrix-assisted laser desorption/ionization (MALDI)-imaging mass...
Cell encapsulating poly(ethylene glycol) hydrogels represent a promising approach for constructing 3D cultures designed to more closely approximate in vivo tissue environment. Improved strategies are needed, however, optimally balance hydrogel permeability support metabolic activities of encapsulated cells, while maintaining patternability restore key aspects architecture. Herein, we have developed one such strategy incorporating hydrophobic nanoparticles partially induce looser...
We have studied the encapsulation of human progenitor cells into 3D PEG hydrogels. Replication-incompetent lentivirus promoter reporter vectors were found to efficiently detect in vivo expression hepatic genes hydrogel-encapsulated liver cells. Similarly, could be infected with hepatitis C virus, and progeny infectious virus recovered from media supernatants Provocatively, diameters these particles range approximately 50 100 nm, while calculated mesh size 8 k hydrogel is 44.6 +/- 1.7 A. To...
We have studied the encapsulation of human progenitor cells into 3D PEG hydrogels. Replication-incompetent lentivirus promoter reporter vectors were found to efficiently detect in vivo expression hepatic genes hydrogel-encapsulated liver cells. Similarly, could be infected with hepatitis C virus, and progeny infectious virus recovered from media supernatants Provocatively, diameters these particles range ∼50 100 nm, while calculated mesh size 8 k hydrogel is 44.6 ± 1.7 Å. To reconcile how...
The temporospatial regulation of axon outgrowth is useful for guiding de novo connectivity or re-connectivity neurons in neurological injury disease. Here we report the successful construction a biocompatible guidance device, which linear propagation IGF-1 gradient sequentially directs outgrowth. We observe extensive vitro axonal extension over 5 mm with desired growth rate ∼ 1 mm/day.
Here we describe the development of a rapid and facile method for colorimetric detection penicillin antibiotics, including G, V, nafcillin, oxacillin, cloxacillin dicloxacillin, using hybrid magnetic nanoparticle clusters (HMNCs) class-selective, antibody-functionalized platinum nanoparticles (Ab-Pt). Amine-functionalized HMNCs were used to chemically capture antibiotics from sample solution through formation amide bonds between amine groups carboxyl antibiotics. Ab-Pt was then allowed bind...
The spatial cell distribution is one of the critical features for governing cellular interactions and their consequent behaviors. Here we suggest a novel method building hierarchical structure by stacking cellattached microplate structures with specific configurations within hydrogel layers. This applied to reconstruction 3D architecture liver lobule development an experimental model various phases cancer angiogenesis.
Abstract Tissue-specific patterned stem cell differentiation serves as the basis for development, remodeling and regeneration of multicellular structure native tissues. We herein proposed a cytocompatible 3D casting process to recapitulate this reconstructing tissues in vitro . first reconstituted 2D culture conditions fate control within hydrogel by incorporating sets diffusible signal molecules delivered through drug-releasing microparticles. Then, utilizing thermo-responsivity...
Abstract A new choked flow criterion based on the system eigenvalues derived by characteristic analysis of a hyperbolic nonequilibrium two-fluid model is employed in RELAP5/MOD3 code. modification produced earlier works Trapp and Ransom elaborated so that better predictions rate can be made two-phase bubbly flow. Marviken tests large-scale break pipes are assessed using present criterion. The assessment results demonstrate more accurate regime without any adjustment than those calculations...
As a part of an ongoing effort to discover inhibitors the Hepatitis C Virus RNA replication, we describe here first synthetic route 1'(<TEX>$\alpha$</TEX>),2'(<TEX>$\beta$</TEX>)-C-dimethyl carbocyclic adenine analogue. The key intermediate cyclopentenyl alcohol 8(<TEX>$\alpha$</TEX>) was prepared from aldehyde 4 using ring-closing metathesis (RCM) as reaction. Coupling with nucleosidic base via regioselective Mitsunobu reaction followed by stereoselective dihydoxylation and deprotection...
The structural integrity of butt fusion (BF) joints in thermoplastic pressure piping systems is critical to their long-term safe use. tapered waist tensile (TWT) specimen was developed alleviate issues associated with ISO 13953 waisted (WT) for evaluating BF joints. Experimental and finite element analyses were performed obtain optimum TWT designs the joint destructive test. For specimens, depending on pipe size, displacement at onset necking reduced by 30~100%, tested area increased 60~80%...