A5066653364- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Microbial Community Ecology and Physiology
- Amino Acid Enzymes and Metabolism
- CRISPR and Genetic Engineering
- Microbial Metabolic Engineering and Bioproduction
- Bacillus and Francisella bacterial research
- Probiotics and Fermented Foods
- RNA modifications and cancer
- Polyamine Metabolism and Applications
- Fungal and yeast genetics research
- Parkinson's Disease Mechanisms and Treatments
- Metalloenzymes and iron-sulfur proteins
- Spectroscopy Techniques in Biomedical and Chemical Research
- Pediatric health and respiratory diseases
- Arsenic contamination and mitigation
- Chromium effects and bioremediation
- Antimicrobial Peptides and Activities
- Infection Control and Ventilation
- Advanced Proteomics Techniques and Applications
Kiel University
2013-2023
Institut für Medizinische Informatik, Biometrie und Epidemiologie
2013
Botanic Garden and Botanical Museum Berlin
2013
Universitätsmedizin Göttingen
2005
University of Göttingen
2005
The methanoarchaea Methanosphaera stadtmanae and Methanobrevibacter smithii are known to be part of the indigenous human gut microbiota. Although immunomodulatory effects bacterial commensals have been studied extensively in last decade, impact human's health disease was rarely examined. Consequently, we report here on M. immune cells. Whereas exposure leads substantial release proinflammatory cytokines monocyte-derived dendritic cells (moDCs), only weak activation detected after incubation...
The clustered regularly interspaced short palindromic repeats (CRISPR) system represents a highly adaptive and heritable defense against foreign nucleic acids in bacteria archaea. We analyzed the two CRISPR-Cas systems Methanosarcina mazei strain Gö1. Although belonging to different subtypes (I-B III-B), leaders of both loci are nearly identical. Also, despite many point mutations each array, common hairpin motif was identified by bioinformatics analysis vitro structural probing. expression...
ABSTRACT In spite of the significant impact biomethylation on mobility and toxicity metals metalloids in environment, little is known about biological formation these methylated metal(loid) compounds. While element-specific methyltransferases have been isolated for arsenic, striking versatility methanoarchaea to methylate numerous metal(loid)s, including rare elements like bismuth, still not understood. Here, we demonstrate that same metal(loid)s (arsenic, selenium, antimony, tellurium,...
ABSTRACT A novel archaeal lytic virus targeting species of the genus Methanosarcina was isolated using mazei strain Gö1 as host. Due to its spherical morphology, designated Met hanosarcina s pherical v irus (MetSV). Molecular analysis demonstrated that MetSV contains double-stranded linear DNA with a genome size 10,567 bp containing 22 open reading frames (ORFs), all oriented in same direction. Functions were predicted for some these ORFs, i.e., such polymerase, ATPase, and DNA-binding...
Summary PII‐like signal transduction proteins are found in all three domains of life and have been shown to play key roles the control bacterial nitrogen assimilation. This communication reports first target protein an archaeal protein, representing a novel PII receptor. The GlnK 1 methanogenic archaeon Methanosarcina mazei strain Gö1 interacts forms stable complexes with glutamine synthetase (GlnA ). Complex formation absence metabolites inhibits activity GlnA . On other hand, this enzyme...
HMm is the only archaeal histone in Methanosarcina mazei Göl and recombinant HMm, synthesized by expression of MM1825 Escherichia coli, has been purified confirmed to have DNA binding compaction properties characteristic an histone. Insertion a puromycin resistance conferring cassette (pac) into was not lethal but resulted mutants (M. MM1825::pac) that impaired ability grow on methanol trimethylamine. Loss also increased sensitivity UV light decreased transcript levels for approximately 25%...
ABSTRACT As members of the indigenous human microbiota found on several mucosal tissues, Methanobrevibacter smithii and Methanosphaera stadtmanae are exposed to effects antimicrobial peptides (AMPs) secreted by these epithelia. Although molecular AMPs bacteria well described, data for archaea not available yet. Besides, it is clear whether affect them as archaeal cell envelope differs profoundly in terms chemical composition structure from that bacteria. The different synthetic growth M. ,...
Small ORF (sORF)‐encoded small proteins have been overlooked for a long time due to challenges in prediction and distinguishing between coding‐ noncoding‐predicted sORFs their biochemical detection characterization. We report on the first functional characterization of protein (sP26) archaeal model organism Methanosarcina mazei, comprising 23 amino acids. The corresponding encoding leaderless mRNA (spRNA26) is highly conserved nucleotide level as well coded acids within numerous strains...
Short open reading frame-encoded peptides (SEP) have been identified across all domains of life and are predicted to be involved in many biochemical processes, however, for the vast majority SEP their biological function is still unknown. Optimized methodologies used mass spectrometric analysis SEP, because traditional methods bottom-up proteomics show a bias against small proteins. Here, different staining SDS-PAGE gels prior in-gel digestion following LC-MS/MS identification archaeon...
We report here on the formation of a complex between two NrpR homologs present in Methanosarcina mazei Gö1 and their binding properties to nifH glnK(1) promoters. Reciprocal co-chromatography demonstrated that NrpRI forms stable complexes with NrpRII (at an : molar ratio ∼ 1 3), which are not affected by 2-oxoglutarate. Promoter-binding, analyses using DNA-affinity chromatography electrophoretic gel mobility shift assays, verified is able bind either promoter or except when NrpRI. Specific...
Archaeosine (G+) is a structurally complex modified nucleoside found quasi-universally in the tRNA of Archaea and located at position 15 dihydrouridine loop, site not any outside G+ characterized by an unusual 7-deazaguanosine core structure with formamidine group 7-position. The location 15, coupled its novel molecular structure, led to hypothesis that stabilizes tertiary through several distinct mechanisms. To test whether contributes stability define biological role G+, we investigated...
With the aim of unraveling their potential involvement in regulation nitrogen metabolism Methanosarcina mazei strain Gö1, we characterized five genes that are differentially transcribed response to changing availability and encoding putative transcriptional regulators. Study respective mutant strains under nitrogen-limited conditions revealed a growth delay for M. MM0444::pac MM1708::pac, strongly reduced diazotrophic MM0872::pac, whereas absence MM2441 or MM2525 did not affect behaviour....
Today, the number of known viruses infecting methanogenic archaea is limited. Here, we report on a novel lytic virus, designated Blf4, and its host strain Methanoculleus bourgensis E02.3, archaeon belonging to Methanomicrobiales, both isolated from commercial biogas plant in Germany. The virus consists an icosahedral head 60 nm diameter long non-contractile tail 125 length, which consistent with new isolate Siphoviridae family. Electron microscopy revealed that Blf4 attaches vegetative cells...
The clustered regularly interspaced short palindromic repeat (CRISPR) system is a prokaryotic adaptive defense against foreign nucleic acids. In the methanoarchaeon Methanosarcina mazei Gö1, two types of CRISPR-Cas systems are present (type I-B and type III-C). Both loci encode Cas6 endonuclease, Cas6b-IB Cas6b-IIIC, typically responsible for maturation functional CRISPR RNAs (crRNAs). To evaluate potential cross cleavage activity, we biochemically characterized both Cas6b proteins regarding...
Abstract The mature 5′‐ends of tRNAs are generated by RNase P in all domains life. ancient form the enzyme is a ribonucleoprotein consisting catalytic RNA and one or more protein subunits. However, hyperthermophilic bacterium Aquifex aeolicus close relatives, protein‐only single type polypeptide (Aq_880, ~23 kDa). In many archaea, homologs Aq_880 were identified (termed HARPs for Homologs P) addition to RNA‐based P, raising question about functions HARP classical these archaea. Here we...
spherical virus (MetSV), infecting
Abstract Patients with Parkinson’s disease (PD) exhibit differences in their gut microbiomes compared to healthy individuals. Although have most commonly been described the abundances of bacterial taxa, changes viral and archaeal populations also observed. Mechanistic links between microbes PD pathogenesis remain elusive but could involve molecules that promote α-synuclein aggregation. Here, we show 2-hydroxypyridine (2-HP) represents a key molecule for PD. We observe significantly elevated...
The linear chromosome of the Methanosarcina spherical virus with 10,567 bp exhibits 22 ORFs mostly unknown functions. Annotation using common tools and databases predicted functions for a few genes like type B DNA polymerase (MetSVORF07) or small (MetSVORF15) major (MetSVORF16) capsid proteins. For verification assigned additional ORFs, biochemical genetic approaches were found to be essential. Consequently, we established system MetSV by cloning its genome into E. coli plasmid pCR-XL-2....
Raman microspectroscopy has been used to thoroughly assess growth dynamics and heterogeneity of prokaryotic cells, yet little is known about how the chemistry individual cells changes during infection with virulent viruses, resulting in so-called virocells. Here, we investigate biochemical bacterial archaeal three different species laboratory cultures before after addition their respective viruses using single-cell microspectroscopy. By applying multivariate statistics, identified...