Abstract BACKGROUND Mulberry leaf protein (MLP) is a high‐quality with significant nutritional value and functional properties. Enzymatic modification of proteins can enhance their properties by using proteases to covalently crosslink or hydrolyze proteins. This study investigates the potential transglutaminase (TGase)‐induced crosslinked MLP as an emulsifier in formation high‐internal‐phase Pickering emulsions. RESULTS Crosslinked samples were prepared TGase concentrations ranging from 0 25...

A computational DFT B3LYP method with 6-311G (d,p) basis set, the in vitro chemical-based and cellular antioxidant activity (CAA) assays were applied this study to explain structure–antioxidant relationships of delphinidin petunidin. The compound molecular structures, spectral properties, frontier orbital energy, transition state petunidin compared. In state, result active site (O21–H32 O22–H33) was consistent bond length. theory results indicated that probable order (0.09126 a.u.) >...

Hovenia acerba Lindl. is a perennial tree of Rhamnaceae family. In the present study, four solvents (80% methanol, ethyl acetate, n-hexane, and water) were used to extract compounds peduncles (HAP). The total contents phenolics tannins (TPC TTC), as well their antioxidant activities (DPPH, ABTS, FRAP) these different extracts including 80% methanol (ME), acetate (EE), n-hexane (HE) water (WE) compared. HAP showed positive correlations with TPC TTC, indicating that maybe major contributor...

Abstract To derive a mutant of L. helveticus SH2‐1 with the capacity weak postacidification and high texturing, first, taking delbrueckii frs4‐1 S. thermophilus grx02 as controls, H + ‐ATPase activity was demonstrated to be highly related SH2‐1. Then, by detecting activity, postacidify (renamed sh2‐5–66) selected from 80 UV mutants. The pH acidity milk fermented sh2‐5–66 were separately 0.57 units higher 57.1 °T lower than that acidification further genetically stable during 100 generations...

Flavonoids (quercetin, luteolin) and carotenoids (lycopene, lutein) were combined at different molecular ratios in a total concentration of 8 μM to investigate their antioxidant interactions. Cellular uptake carotenoids, the expression carotenoid transporters, ROS scavenging ability, enzymes activities compared HUVEC, Caco-2, L-02 cells. Combinations with flavonoids majority showed stronger activity. Lycopene quercetin ratio 1:5 activities, increased 18, 12, 12 activity (CAA) units cells,...

The hydrophilic extracts of mulberry (HEM) and blueberry (HEB) lipophilic mango (LEM) watermelon (LEW) were mixed in different ratios to assess the antioxidant interactions by chemical-based (DPPH ABTS assays) H9c2 cell-based models. There both synergistic antagonistic among these fruits. Some groups with combinational showed stronger effects than individual groups, others (HEM-LEW F1/10, LEW-LEM F5/10, HEB-LEM F3/10) based on indicators [the values DPPH, ABTS, MTT; expression superoxide...

Oxidative stress is a potential factor in the promotion of endothelial dysfunction. In this research, flavonoids (quercetin, luteolin) combined with carotenoids (lycopene, lutein), especially quercetin-lycopene combination (molar ratio 5:1), prevented oxidative HUVEC cells by reducing reactive oxygen species (ROS) and suppressing expression NADPH oxidase 4 (Nox4), major source ROS production. RNA-seq analysis indicated downregulated inflammatory genes induced H2O2, such as IL-17 NF-κB. The...

Pathogenic microorganisms have been detected in fermented food. Combining the enormous class of pathogens and their continuously appearing mutants or novel species, it is important to select suitable safe antibacterial agents for food safety. Lactic acid bacteria (LAB) which produce diverse imperative antimicrobial metabolites an immense number applications industry. Here, human-derived strain YT was isolated due its cell-free supernatant (CFS-YT) cells (Cs-YT), respectively performed...

Introduction Kefir grains with efficient proteolytic system is an excellent starter culture for the production of bioactive peptides and milk products. This study explores casein derived from fermented bovine by kefir using peptidomics approaches. The angiotensin converting enzyme (ACE) inhibitory activity these were also investigated. Methods After fermentation, based on LC-MS/MS was used to investigate dynamic profile structure specificity generated peptides. ACE determined measuring...

Abstract BACKGROUND To improve the characteristics and safety of traditional cuocai, selection use mixed starter cultures is important. This study aimed to isolate main indigenous lactic acid bacteria (LABs) yeast species from spontaneous fermented mustard leaves, they were used as ferment leaves. RESULTS Five predominant LABs, namely Q‐1 ( Enterococcus faecalis ), G‐1 Lactobacillus plantarum G‐3 pentosus G‐2 G‐4 buchneri ) strains, two strains J‐1 Issatchenkia orientalis J‐2 occidentalis...

Purpose: To assess the suitability of high performance liquid chromatography (HPLC) for determination cordycepin content Cordyceps militaris recombinant riceMethods: rice was made by mixing brown with artificial Chinese caterpillar fungus culture medium powder using twin-screw extrusion technology. Cordycepin determined reversed-phase HPLC water:acetonitrile (95:5, v/v) as mobile phase, detection wavelength 260 nm, and flow rate 1.0 mL/min.Results: contents showed good linearity in range 1 -...

The antioxidant activities of lycopene (LY), lutein (LU), chlorogenic acid (CA), and delphinidin (DP) were tested in vitro on H9c2 cell-based models. Some indicators, such as the generation reactive oxygen (ROS), quantification cell activity (CAA), expressions SOD, GSH-Px, CAT, calculated to examine their interactions. From our results, phytochemical mixtures (M1: CA-LU: F3/10, M2: DP-CA: F7/10, M3: DP-LY: F5/10) displayed strong synergistic effects based ROS CAA. However, great antagonistic...

Quercetin and lycopene are strong dietary antioxidants that often co-exist in foods. Here, the cardiac neuroprotective effects of quercetin combination were analyzed D-galactose-induced oxidative stress mice. ICR mice divided into control group, D-galactose group (D-GAL), treatment groups (Q45: 45 mg·kg−1·d−1quercetin, Q60: 60 mg·kg−1·d−1 quercetin), (15 lycopene), M (45 mg·kg−1·d−1quercetin+15 lycopene). Mice injected with (150 mg·kg−1·d−1) for 6 weeks except group. Results showed...