A5016147352- Ubiquitin and proteasome pathways
- Prion Diseases and Protein Misfolding
- Adenosine and Purinergic Signaling
- Obesity, Physical Activity, Diet
- Cancer, Hypoxia, and Metabolism
- Health and Lifestyle Studies
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Gastroesophageal reflux and treatments
- Protein Degradation and Inhibitors
- Cancer-related Molecular Pathways
- Eosinophilic Esophagitis
- Biochemical and Molecular Research
- Cultural Identity and Heritage
- Advanced Proteomics Techniques and Applications
- RNA Research and Splicing
- Helicobacter pylori-related gastroenterology studies
- Fungal and yeast genetics research
- Diet, Metabolism, and Disease
- Studies on Chitinases and Chitosanases
- Physical Activity and Health
- Neurological diseases and metabolism
- Microbial Metabolic Engineering and Bioproduction
- Obesity and Health Practices
- Genetics and Neurodevelopmental Disorders
Max Planck Institute of Biochemistry
2016-2023
McMaster University
2016-2022
Impact
2017-2022
Brown University
2012-2016
University of Arizona
2016
John Brown University
2014
Albert Einstein College of Medicine
2012
Canadian Institutes of Health Research
2008-2009
Université de Sherbrooke
2008-2009
Abstract Epithelial cells participate in the immune response of intestinal mucosa. Extracellular nucleotides have been recognized as inflammatory molecules. We investigated role extracellular and their associated P2Y receptors secretion cytokines by epithelial cells. The effect inflammation on P2Y6 receptor expression was determined PCR mouse, rat, human. Localization immunofluorescence microscopy colon normal dextran sulfate sodium-treated mice. activation UDP cytokine release using a...
Abstract Inflammatory stresses associated with inflammatory bowel diseases up-regulate P2Y2 mRNA receptor expression in the human colon adenocarcinoma cell line Caco-2, noncancerous IEC-6 cells and colonic tissues of patient suffering from Crohn’s disease ulcerative colitis. However, transcriptional events regulating (P2Y2R) are not known. We have identified a putative transcription start site P2Y2R gene demonstrated acetylation Lys14 on histone H3 Lys8 H4, thus suggesting that chromatin...
The proteostasis network has evolved to support protein folding under normal conditions and expand this capacity in response proteotoxic stresses. Nevertheless, many pathogenic states are associated with misfolding, revealing vivo limitations on quality control mechanisms. One contributor these is the physical characteristics of misfolded proteins, as exemplified by amyloids, which largely resistant clearance. However, other imposed cellular environment poorly understood. To identify...
Article19 April 2022Open Access Transparent process A GID E3 ligase assembly ubiquitinates an Rsp5 adaptor and regulates plasma membrane transporters Christine R Langlois Corresponding Author [email protected] orcid.org/0000-0003-1567-3577 Department of Molecular Machines Signaling, Max Planck Institute Biochemistry, Martinsried, Germany Contribution: Conceptualization, Data curation, Formal analysis, Supervision, Investigation, Methodology, Writing - original draft, review & editing Search...
Ubiquitylation is catalyzed by coordinated actions of E3 and E2 enzymes. Molecular principles governing many important E3-E2 partnerships remain unknown, including those for RING-family GID/CTLH ubiquitin ligases their dedicated E2, Ubc8/UBE2H (yeast/human nomenclature). GID/CTLH-Ubc8/UBE2H-mediated ubiquitylation regulates biological processes ranging from yeast metabolic signaling to human development. Here, cryoelectron microscopy (cryo-EM), biochemistry, cell biology reveal this...
Prions are a special class of proteins capable adopting multiple (misfolded) conformations, some which have been associated with fatal diseases in mammals such as bovine spongiform encephalopathy or Creutzfeldt–Jakob Disease. Prion diseases, like protein misfolding general, caused by the formation and amplification ordered aggregates called amyloids. While can take decades to form, yeast variety prion phenotypes that occur over few hours, making this system an ideal model for disease...
Significance We use a single-run, data-independent acquisition–based mass spectrometry approach to generate and compare dozens of yeast proteomes in less than day, provide comprehensive resource detailing changes the proteome following commonly used stress treatments yeast. Our systems biology identifies validates regulatory targets an E3 ubiquitin ligase during metabolic switch, providing insights into interplay pathways. The speed, simplicity, scalability this workflow makes it...
In intestine, neutrophils are recruited in response to bacterial infiltration and their anti-cellular activities contribute inflammatory bowel diseases. contrast, little is known regarding the recruitment of MPhi intestinal epithelium. Extracellular adenosine uridine 5'-triphosphate (ATP UTP) can function as leukocyte chemoattractants. We investigated effects these nucleotides on ability epithelial cells (IEC) promote transepithelial migration adhesion. ATP UTP promoted neutrophil-like...
Prions are a group of proteins that can adopt spectrum metastable conformations in vivo. These alternative states change protein function and self-replicating transmissible, creating protein-based elements inheritance infectivity. Prion conformational flexibility is encoded the amino acid composition sequence protein, which dictate its ability not only to form an ordered aggregate known as amyloid but also maintain transmit this structure But, while we effectively predict propensity vitro,...
Abstract Genome wide association studies (GWAS) have identified single-nucleotide polymorphisms (SNPs) that are associated with fasting plasma glucose (FPG) in adult European populations. The contribution of these SNPs to FPG non-Europeans and children is unclear. We studied the 15 GWAS a genotype score (GS) 7 metabolic traits 1,421 Mexican adolescents from Mexico City. Genotyping was performed using TaqMan Open Array. used multivariate linear regression models adjusted for age, sex, body...
Introduction Obesity is a global epidemic and risk factor for developing other comorbidities. Young adulthood critical period body weight change establishing healthy lifestyle behaviours. The ‘Freshman 15’ suggests that undergraduate students gain 15 lbs (6.8 kg) during their first year of university, although evidence estimates more modest approximately 3–5 (1.4–2.3 kg). Previous studies have only investigated in the do not study potential factors. Genetic EnviroNmental Effects on...
The transition to university often involves a change in living arrangement for many first-year students. While weight gain during first year of has been well documented, Canadian literature on the impact within this context is limited. objective investigation was explore effect anthropometric traits students from Ontario, Canada.244 undergraduate were followed longitudinally with data collected early academic and towards end year. Anthropometric parameters including weight, waist hip...
Article The Effects of Pubertal Status and Glycemic Control on the Growth Hormone-IGF-I Axis in Boys with Insulin-Dependent Diabetes Mellitus was published May 1, 1998 journal Journal Pediatric Endocrinology Metabolism (volume 11, issue 3).
Abstract Cells rapidly remodel their proteomes to align cellular metabolism environmental conditions. Ubiquitin E3 ligases enable this response, by facilitating rapid and reversible changes protein stability, localization, or interaction partners. In S. cerevisiae , the GID ligase regulates switch from gluconeogenic glycolytic conditions through induction incorporation of substrate receptor subunit Gid4, which promotes degradation enzymes. Here, we show an alternative receptor, Gid10, is...
Background Little is known about the impact of race/ethnicity on weight change at university. The objective this study to determine if ethnicity has an obesity traits in a multiethnic cohort first-year students McMaster University Ontario, Canada. Methods 183 first year from three most represented ethnic groups (South Asian, East and white-Caucasian) our sample were followed longitudinally with data collected early academic towards end year. Obesity parameters including body weight, mass...
Background While weight gain during first year of university has been well documented in North America, literature on sex-specific effects is scarce and inconsistent. The objective this investigation was to explore changes obesity traits at McMaster University (Ontario, Canada). Methods 245 first-year students (80.4% females) were followed longitudinally with data collected early the academic towards end year. Obesity parameters including weight, waist hip circumferences, BMI, ratio...
SUMMARY Cells respond to environmental changes by toggling metabolic pathways, preparing for homeostasis, and anticipating future stresses. For example, in Saccharomyces cerevisiae , carbon stress-induced gluconeogenesis is terminated upon glucose availability, a process that involves the multiprotein E3 ligase, GID SR4 recruiting N-termini catalyzing ubiquitylation of gluconeogenic enzymes. Here, genetics, biochemistry, cryo electron microscopy define molecular underpinnings g lucose- i...
To explore the patterns and predictors of body mass index (BMI) change among undergraduate students from Ontario (Canada).