A5086217955- Prostate Cancer Treatment and Research
- Telomeres, Telomerase, and Senescence
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Prostate Cancer Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Cancer, Lipids, and Metabolism
- CRISPR and Genetic Engineering
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA modifications and cancer
- Genetics, Aging, and Longevity in Model Organisms
- Bioinformatics and Genomic Networks
- Sexual Differentiation and Disorders
- Urologic and reproductive health conditions
- Cancer Genomics and Diagnostics
- IgG4-Related and Inflammatory Diseases
- Kidney Stones and Urolithiasis Treatments
- Mechanisms of cancer metastasis
- Smart Materials for Construction
- Flood Risk Assessment and Management
- Biomedical Research and Pathophysiology
- Trypanosoma species research and implications
- Long-Term Effects of COVID-19
Universidade Federal de São Paulo
2025
University of Birmingham
2022-2024
Universidade de São Paulo
2021-2024
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2020-2024
Genomics (United Kingdom)
2023-2024
D’Or Institute for Research and Education
2021-2023
Park University
2020
Seattle University
2020
Universidade Estadual Paulista (Unesp)
2020
Université de Tours
2019
Abstract Ageing is a complex and multifactorial process. For two decades, the Human Genomic Resources (HAGR) have aided researchers in study of various aspects ageing its manipulation. Here, we present key features recent enhancements these resources, focusing on six main databases. One database, GenAge, focuses genes related to ageing, featuring 307 linked human 2205 associated with longevity model organisms. AnAge longevity, life-history across animal species, containing data 4645 species....
COVID-19 represents a public health emergency, whose mechanism of which is not fully understood. It speculated that microRNAs may play crucial role in host cells after infection by SARS-CoV-2. Thus, our study aimed to analyze the expression miR-200c-3p saliva samples from patients with COVID-19. One handred eleven were divided into 4 groups. Group I: 39 negative for Covid-19; II: 37 positive and symptomatic patients, no indication hospitalization; III: 21 respiratory disorders...
<title>Abstract</title> Forest fires produce toxic emissions that are detrimental to human health. Currently, the Pantanal, largest continuous wetland in world, is facing intense fires. This situation may contribute increased hospital admissions for respiratory and cardiovascular diseases. To assess additive effect between active fire hotspots hospitalizations of residents a generalized linear model was used, incorporating geolocalized covariates such as air quality climatic conditions. Our...
The infection by COVID-19 is a serious global public health problem. An efficient way to improve this disease's clinical management would be characterize patients at higher risk of progressing critically severe using prognostic biomarkers. telomere length could used for purpose. Telomeres are responsible controlling the number maximum cell divisions. biomarker aging and several diseases. We aimed compare leukocyte (LTL) between without with different severity infection. Were included 53 who...
Prostate cancer (PCa) has a high prevalence and represents an important health problem, with increased risk of metastasis. With the advance CRISPR-Cas9 genome editing, new possibilities have been created for investigating PCa. The technique is effective in knockout oncogenes, reducing tumor resistance. MMP9 miR-21 target genes are associated PCa progression; therefore, we evaluated MMP-9 targets using system. Single guide RNAs (sgRNAs) sequences were inserted into PX-330 plasmid, transfected...
specialists have an urge for biomarkers that can discriminate indolent prostate cancer from aggressive tumors. Ki67 is a proliferation marker, and its expression associated with the aggressiveness of several cancers.analyze in samples correlating disease.Ki67 mRNA levels were determined utilizing data TCGA cohort (Tumor(n)=492 control(n)=52). The protein was on 94 biopsies patients by immunohistochemical assay.in mRNA, upregulation tissue (p<0.0001) worst disease-free survival (p=0.035)....
Abstract Introduction Understanding changes in cell identity cancer and ageing is of great importance. In this work, we analyzed how gene expression human tissues are associated with tissue specificity during using transcriptome data from TCGA GTEx. Results We found significant downregulation tissue-specific genes 40% the analyzed, which suggests loss age. For most types, have noted a consistent pattern that specific to tumor originated. Moreover, observed an activation not usually expressed...
BACKGROUND/AIMS: Cholesterol modulates intratumoral androgenic signaling in prostate cancer; however, the molecular mechanisms underlying these changes castration-resistant cancer (CRPC) are not fully elucidated. Herein, we investigated effect of cholesterol on androgen receptor (AR) coactivators expression and tumorigenesis vitro vivo. METHODS: monitored AR (SRC-1, 2, 3 PCAF) genes PC-3 cells exposed to 2µg/mL for 8 hours by qPCR. We also performed cell migration at 0, 8, 24, 48 72h flow...
MicroRNAs (miRNAs) have gained a prominent role as biomarkers in prostate cancer (PCa). Our study aimed to evaluate the potential suppressive effect of miR-137 model advanced PCa with and without diet-induced hypercholesterolemia. In vitro, PC-3 cells were treated 50 pmol mimic for 24 h, gene protein expression levels SRC-1, SRC-2, SRC-3, AR evaluated by qPCR immunofluorescence. We also assessed migration rate, invasion, colony-forming ability, flow cytometry assays (apoptosis cell cycle)...
Peyronie's disease (PD) is characterized by the formation of fibrous plaque in tunica albuginea, causing several problems patients. The etiology this not fully understood, and there are few effective treatments. To better understand molecular pathways PD, we studied miR-29b, a microRNA that could be involved with illness. MicroRNAs endogenous molecules act inhibiting messenger RNA. MiR-29b regulates 11 20 collagen genes TGF-β1 gene, which related to PD progression.We compared miR-29b...
Abstract Ageing is a complex and multifactorial process. For two decades, the Human Genomic Resources (HAGR) have aided researchers in study of various aspects ageing its manipulation. Here we present key features recent enhancements these resources, focusing on six main databases. One database, GenAge, focuses genes related to ageing, featuring 307 linked human 2205 associated with longevity model organisms. AnAge longevity, life-history across animal species, containing data 4645 species....
The new identified protein telomeric zinc-finger associated (TZAP) is a negative regulator of telomere length. Since length and maintenance mechanisms are essential to cancer progression, TZAP considered player in biology. Here we aimed analyze using the Cancer Genome Atlas data Pan-Cancer approach. We gathering from TCGA studies utilizing cBioPortal, GEPIA UALCAN. In total analyzed 33 types (n=9664) their respective controls (n=711). transcribed all cancers but less than 5% tumors show any...
Abstract Background Telomere dysfunction is one of the hallmarks cancer and crucial to prostate carcinogenesis. TERF1 a gene essential telomere maintenance, its has already been associates with several cancers. target miR-155, this microRNA can inhibit expression promotes carcinogenesis in breast cancer. We aim analyze TERF1, mRNA level, involvement progression. Results Alterations DNA were evaluated using datasets primary tumor castration-resistant tumors (CRPC) deposited cBioportal. The...
Prostate cancer is the most frequent malignancy affecting men worldwide. Due to low sensitivity and specificity of prostate-specific antigen test digital rectal exam as screening modalities, several alternatives are being studied. This study aimed evaluate application MMP-9 its regulators (TIMP-1, RECK, miR-338-3p) diagnostic prognostic indicators prostate cancer. A total 134 randomly selected patients under investigation for submitted a transrectal ultrasound-guided biopsy were enrolled in...
Abstract Purpose To assess the role of p160 family, AR, and AR-V7 in different initial presentations prostate cancer their association with clinical endpoints related to tumor progression. Methods The study sample comprises 155 patients who underwent radical prostatectomy 11 healthy peripheral zone biopsies as control group. Gene expression was quantified by qPCR from tissue specimens. statistical analysis investigated correlations between gene levels, associations disease presence,...
Despite their biological importance, the role of stem cells in human aging remains to be elucidated. In this work, we applied a machine learning methodology GTEx transcriptome data and assigned stemness scores 17,382 healthy samples from 30 tissues aged between 20 79 years. We found that ~60% studied exhibit significant negative correlation subject's age score. The only exception was uterus, where observed an increased with age. Moreover, is positively correlated cell proliferation...
Introdução: Os tumores de células germinativas testiculares representam cerca 97% dos cânceres testiculares. Histologicamente, classificam-se em seminomas e não seminomas, tendo aplicabilidade diagnóstica prognóstica. O sucesso terapêutico depende do diagnóstico precoce associado ao correto estadiamento, sendo então grande importância a avaliação biomarcadores que possam contribuir para o manejo dessa doença. Objetivo: Identificar os genes podem estar correlacionados com prognóstico...
Abstract Background Peyronie's disease is characterized by the formation of fibrotic plaques in penile tunica albuginea. Effective treatments are limited, warranting investigation new promising therapies, such as application microRNAs that regulate fibrosis‐related genes. Objective We aimed to investigate therapeutic potential mimicking microRNA‐29b a fibrin‐induced rat model disease. Material/methods The study was designed two phases. To establish an optimal model, rats received either...