A5012458582- Bladder and Urothelial Cancer Treatments
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Prostate Cancer Treatment and Research
- Telomeres, Telomerase, and Senescence
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- NF-κB Signaling Pathways
- Immune cells in cancer
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Urological Disorders and Treatments
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
- Cancer-related Molecular Pathways
- Metabolism, Diabetes, and Cancer
- Adipose Tissue and Metabolism
- Sexual function and dysfunction studies
- Sexual Differentiation and Disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Peptidase Inhibition and Analysis
- Fibroblast Growth Factor Research
- FOXO transcription factor regulation
- Diabetes Treatment and Management
- Immune responses and vaccinations
Universidade de São Paulo
2018-2024
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2021-2024
Universidade Estadual de Campinas (UNICAMP)
2014-2023
University of Aveiro
2023
Centro Universitário das Faculdades Metropolitanas Unidas
2021
Brazilian Center for Research in Energy and Materials
2018
Pontifícia Universidade Católica de Campinas
2014-2017
Associação Fundo de Incentivo à Pesquisa
2014
Laboratoire de Chimie Physique et Microbiologie pour l'Environnement
2012
Metformin is the most prescribed drug for DM2, but its site and mechanism of action are still not well established. Here, we investigated effects metformin on basolateral intestinal glucose uptake (BIGU), consequences hepatic production (HGP). In diabetic patients mice, primary was gut, increasing BIGU, evaluated through PET-CT. mice CaCo2 cells, this increase in BIGU resulted from an GLUT1 GLUT2, secondary to ATF4 AMPK. hyperglycemia, increased lactate (reducing pH bicarbonate portal vein)...
To investigate the effect of probiotic supplementation during development an experimental model colitis associated colon cancer (CAC).C57BL/6 mice received intraperitoneal injection azoxymethane (10 mg/kg), followed by three cycles sodium dextran sulphate diluted in water (5% w/v). Probiotic group daily a mixture Lactobacillus acidophilus, rhamnosus and Bifidobacterium bifidum. Microbiota composition was assessed 16S rRNA Illumina HiSeq sequencing. Colon samples were collected for...
COVID-19 represents a public health emergency, whose mechanism of which is not fully understood. It speculated that microRNAs may play crucial role in host cells after infection by SARS-CoV-2. Thus, our study aimed to analyze the expression miR-200c-3p saliva samples from patients with COVID-19. One handred eleven were divided into 4 groups. Group I: 39 negative for Covid-19; II: 37 positive and symptomatic patients, no indication hospitalization; III: 21 respiratory disorders...
The acquisition of a castration-resistant prostate cancer phenotype by cells is the alteration that has worst prognosis for patients. aim this study was to evaluate role microRNAs-23b/-27b as well possible CCNG1 target gene in tissue samples from patients with localized progressed and cell line (PC-3). microRNAs expression levels surgical specimens were analyzed quantitative real-time polymerase chain reaction. line, PC-3, transfected pre-miR-23b, pre-miR-27b, their respective controls using...
The infection by COVID-19 is a serious global public health problem. An efficient way to improve this disease's clinical management would be characterize patients at higher risk of progressing critically severe using prognostic biomarkers. telomere length could used for purpose. Telomeres are responsible controlling the number maximum cell divisions. biomarker aging and several diseases. We aimed compare leukocyte (LTL) between without with different severity infection. Were included 53 who...
The use of natural products and derivatives for the prevention control non-communicable chronic diseases, such as type-2 diabetes (T2D), obesity, hepatic steatosis is a way to achieve homeostasis through different metabolic pathways. Thus, male C57BL/6 mice were divided into following groups: high-fat diet (HFD) vehicle, HFD + Supplemented, Supplemented_S, isolated compounds. vehicle experimental formulations administered orally by gavage once day over four weeks (28 consecutive days). We...
Phospholipase A2 (PLA2) is a large family of enzymes involved in the inflammatory process that catalyzes hydrolysis membrane phospholipids, leading to production free fatty acids and lysophospholipids, starting arachidonic acid cascade. Their expression has been related behavior several cancers. Our objective search for PLA2 prostate cancer (PCa) tissue correlates with prognosis survival.
Aging | doi:10.18632/aging.101138. Vagner Ramon Rodrigues Silva, Carlos Kiyoshi Katashima, Carla G. Bueno Luciene Lenhare, Thayana Oliveira Micheletti, Rafael Ludemann Camargo, Ana Carolina Ghezzi, Juliana Alves Alexandre Moura Assis, Natalia Tobar, Joseane Morari, Daniela S. Razolli, Leandro Pereira Moura, José Rodrigo Pauli, Dennys Esper Cintra, Lício Augusto Velloso, Mario J.A Saad, Eduardo Rochete Ropelle
Prostate cancer (PCa) has a high prevalence and represents an important health problem, with increased risk of metastasis. With the advance CRISPR-Cas9 genome editing, new possibilities have been created for investigating PCa. The technique is effective in knockout oncogenes, reducing tumor resistance. MMP9 miR-21 target genes are associated PCa progression; therefore, we evaluated MMP-9 targets using system. Single guide RNAs (sgRNAs) sequences were inserted into PX-330 plasmid, transfected...
Hypothalamic sphingosine‐1‐phosphate receptor 1 (S1PR1), the G protein–coupled of sphingosine‐1‐phosphate, has been described as a modulator in control energy homeostasis rodents. However, this mechanism is still unclear. Here, we evaluate role interleukin 6 (IL‐6) associated with acute physical exercise hypothalamic S1PR1–signal transducer and activator transcription 3 (STAT3) axis. Acute session an intracerebroventricular IL‐6 injection increased S1PR1 protein content STAT3 phosphorylation...
BACKGROUND/AIMS: Cholesterol modulates intratumoral androgenic signaling in prostate cancer; however, the molecular mechanisms underlying these changes castration-resistant cancer (CRPC) are not fully elucidated. Herein, we investigated effect of cholesterol on androgen receptor (AR) coactivators expression and tumorigenesis vitro vivo. METHODS: monitored AR (SRC-1, 2, 3 PCAF) genes PC-3 cells exposed to 2µg/mL for 8 hours by qPCR. We also performed cell migration at 0, 8, 24, 48 72h flow...
MicroRNAs (miRNAs) have gained a prominent role as biomarkers in prostate cancer (PCa). Our study aimed to evaluate the potential suppressive effect of miR-137 model advanced PCa with and without diet-induced hypercholesterolemia. In vitro, PC-3 cells were treated 50 pmol mimic for 24 h, gene protein expression levels SRC-1, SRC-2, SRC-3, AR evaluated by qPCR immunofluorescence. We also assessed migration rate, invasion, colony-forming ability, flow cytometry assays (apoptosis cell cycle)...
We characterized the functional consequences of intravesical bacillus Calmette-Guérin on molecular mechanism AKT/mTOR signaling pathway in nonmuscle invasive bladder cancer. To our knowledge this has not been reported previously.At age 7 weeks female Fischer 344 rats received 1.5 mg/kg MNU intravesically every other week for 6 weeks. They were randomized at 10 per group to (0.2 ml vehicle), (10(6) cfu Connaught strain), rapamycin (15 μg/ml) and plus simultaneous rapamycin, each At 15...
Peyronie's disease (PD) is characterized by the formation of fibrous plaque in tunica albuginea, causing several problems patients. The etiology this not fully understood, and there are few effective treatments. To better understand molecular pathways PD, we studied miR-29b, a microRNA that could be involved with illness. MicroRNAs endogenous molecules act inhibiting messenger RNA. MiR-29b regulates 11 20 collagen genes TGF-β1 gene, which related to PD progression.We compared miR-29b...
Prostate cancer has a high incidence in men and is the second cause of death among americans male. microRNA (miR) becoming potential new prognostic factor for prostate cancer. Single nucleotide polymorphisms (SNPs) are common polymorphisms, characterized by single exchange nitrogen based DNA. This polymorphism present miRs, altering their function.To evaluate role SNP rs1834306 miR100 rs2910164 miR146a development prognosis cancer.One hundred patients diagnosed with 68 controls were...
Radical prostatectomy, a standard management approach for localized Prostate Cancer (PC), may cause stress response associated with immune modulating effects. Regional anesthesia was hypothesized to reduce the effects of surgery by minimizing neuroendocrine surgical response, thus mitigating tumor cells dissemination. Our primary objective investigate whether use spinal blocks attenuates PC dissemination on an animal model. We also assessed number circulating NK and amount inflammatory...
The new identified protein telomeric zinc-finger associated (TZAP) is a negative regulator of telomere length. Since length and maintenance mechanisms are essential to cancer progression, TZAP considered player in biology. Here we aimed analyze using the Cancer Genome Atlas data Pan-Cancer approach. We gathering from TCGA studies utilizing cBioPortal, GEPIA UALCAN. In total analyzed 33 types (n=9664) their respective controls (n=711). transcribed all cancers but less than 5% tumors show any...