Mass spectrometry imaging (MSI) is a powerful tool for label-free mapping of the spatial distribution proteins in biological tissues. We have previously demonstrated individual proteoforms tissues using nanospray desorption electrospray ionization (nano-DESI), an ambient liquid extraction-based MSI technique. Nano-DESI generates multiply charged protein ions, which advantageous their identification top-down proteomics analysis. In this study, we demonstrate proteoform with resolution down to...

Mass spectrometry imaging (MSI) is widely used for examining the spatial distributions of molecules in biological samples. Conventional MSI approaches, which extracted from sample are distinguished based on their mass-to-charge ratio, cannot distinguish between isomeric species and some closely spaced isobars. To facilitate isobar separation, typically performed using high-resolution mass spectrometers. Nevertheless, complexity mixture biomolecules observed each pixel image presents a...

Unraveling the complexity of lipidome requires development novel approaches for structural characterization lipid species with isomer-level discrimination. Herein, we introduce an online photochemical approach isomer identification through selective derivatization double bonds by reaction singlet oxygen. Lipid hydroperoxide products are generated promptly after laser irradiation. Fragmentation these in a mass spectrometer produces diagnostic fragments revealing C=C locations unreacted...

Abstract Unraveling the complexity of lipidome requires development novel approaches for structural characterization lipid species with isomer‐level discrimination. Herein, we introduce an online photochemical approach isomer identification through selective derivatization double bonds by reaction singlet oxygen. Lipid hydroperoxide products are generated promptly after laser irradiation. Fragmentation these in a mass spectrometer produces diagnostic fragments revealing C=C locations...

A multimodal approach that integrates IF and high resolution nano-DESI MSI using image registration segmentation tools to characterize the chemical composition of skeletal fibers.

Mass spectrometry imaging (MSI) enables label-free mapping of hundreds molecules in biological samples with high sensitivity and unprecedented specificity. Conventional MSI experiments are relatively slow, limiting their utility for applications requiring rapid data acquisition, such as intraoperative tissue analysis or 3D imaging. Recent advances technology focus on improving the spatial resolution molecular coverage, further increasing acquisition time. Herein, a deep learning approach...

Spatial lipidomics is a powerful technique for understanding the complexity of lipidome in biological systems through mass spectrometry imaging (MSI). Recent advancements have enabled isomer-selected MSI (iMSI) lipids samples using both online and off-line derivatization strategies. Despite these impressive developments, most iMSI techniques are limited to either positive or negative ion mode analysis, restricting molecular coverage achievable single experiment. Additionally, efficiency...

Objective: Herein, we evaluate the performance of xylazine test strips (XTS) in urine samples. XTS is used for community drug checking (powders and liquids) but lacks regulatory approval human specimen testing. Methods: We obtained n=85 specimens from a toxicology laboratory Philadelphia, originally submitted qualitative mass spectrometry (MS) expanded analysis. Residual was tested using (BTNX Inc.), results were then compared against MS method. Synthetic spiked with standards to determine...

Opioid misuse and overdose have caused devastating public health challenges economic burdens, calling for the need of rapid, accurate sensitive opioid sensors. Here, we report a photonic crystal-based sensor in total internal reflection configuration, providing label-free, quantitative measurements through change refractive index. The one-dimensional crystal with defect layer that is immobilized antibodies acts as resonator an open microcavity. highly accessible structure responds to...

Abuse of fentanyl and its analogs is a major contributor to the opioid overdose epidemic in United States, but detecting quantifying trace amounts such drugs remains challenge without resorting sophisticated mass spectrometry-based methods.

Metals are essential nutrients that all living organisms acquire from their environment. While metals necessary for life, excess metal uptake can be toxic; therefore, intracellular levels tightly regulated in bacterial cells. Staphylococcus aureus, a Gram-positive bacterium, relies on and metabolism to colonize vertebrates. Thus, we hypothesized an expanded understanding of homeostasis S. aureus will lead the discovery pathways targeted with future antimicrobials. We sought identify small...

The design of functional interfaces is central to both fundamental and applied research in materials science energy technology. We introduce a new, broadly applicable technique for the precisely controlled high-throughput preparation well-defined containing polyatomic species ranging from small ions nanocrystals large protein complexes. mass-dispersive deposition onto surfaces achieved using rotating-wall mass analyzer, compact device which enables separation low voltages has theoretically...

Abstract The design of functional interfaces is central to both fundamental and applied research in materials science energy technology. We introduce a new, broadly applicable technique for the precisely controlled high‐throughput preparation well‐defined containing polyatomic species ranging from small ions nanocrystals large protein complexes. mass‐dispersive deposition onto surfaces achieved using rotating‐wall mass analyzer, compact device which enables separation low voltages has...

Unraveling the complexity of lipidome requires development novel approaches for structural characterization lipid species with isomeric resolution. Herein, we introduce an online photochemical approach isomer identification through selective derivatization double bonds by reaction singlet oxygen. Lipid hydroperoxide products are generated promptly after laser irradiation. Fragmentation these in a mass spectrometer produces diagnostic fragments, which reveal C=C locations unreacted lipids....

Unraveling the complexity of lipidome requires development novel approaches for structural characterization lipid species with isomeric resolution. Herein, we introduce an online photochemical approach isomer identification through selective derivatization double bonds by reaction singlet oxygen. Lipid hydroperoxide products are generated promptly after laser irradiation. Fragmentation these in a mass spectrometer produces diagnostic fragments, which reveal C=C locations unreacted lipids....

Se prepararon catalizadores a base de óxidos níquel dopados con titanio Ni1-αTiα O α 0,01 0,05, por un método evaporación controlada 550ºC para la deshidrogenación oxidativa (ODH) etano etileno. La introducción pequeñas cantidades Ti en celda del NiO tuvo efectos notables sobre las propiedades óxido simple níquel, aumentando superficie BET. Además, se observó una disminución tamaño cristal tras el análisis XRD, generación nuevos sitios ácidos, evaluados TPD-NH3 , redistribución especies...

Journal Article Tissue Mass Spectrometry: How Solid Is Our Future? Get access Daisy Unsihuay, Unsihuay Clinical Chemistry Fellow, Department of Pathology and Laboratory Medicine, University Pennsylvania, Philadelphia, PA, United StatesClinical Children’s Hospital States Search for other works by this author on: Oxford Academic Google Scholar William S Phipps, Phipps Assistant Professor, Medicine Pathology, Washington, Seattle, WA, Address correspondence to at: & 1959 NE Pacific St, Box...

Abstract A highly sensitive immunoassay with sub-picogram limit of detection for fentanyl and a wide range analogs has been developed, using novel high affinity antibody fused NanoLuc, small-size luciferase that can emit strong stable luminescence. When applied to unaltered clinical human urine samples, the assay fentanyl, results fully concordant gold standard liquid chromatography-mass spectrometry. environmental detect as low 0.25 pg per inch 2 environment surface. Assay turn-around-time...