A5054820512- Reproductive System and Pregnancy
- Mass Spectrometry Techniques and Applications
- Cannabis and Cannabinoid Research
- Endometriosis Research and Treatment
- Prenatal Substance Exposure Effects
- Reproductive Biology and Fertility
- Pregnancy and preeclampsia studies
- Metabolomics and Mass Spectrometry Studies
- Gynecological conditions and treatments
- Natural product bioactivities and synthesis
- Atrial Fibrillation Management and Outcomes
- Wnt/β-catenin signaling in development and cancer
- Analytical Chemistry and Chromatography
- Preterm Birth and Chorioamnionitis
- Inflammatory mediators and NSAID effects
- Cardiac Arrhythmias and Treatments
- Reproductive Physiology in Livestock
- Microfluidic and Capillary Electrophoresis Applications
- Neonatal Respiratory Health Research
- Birth, Development, and Health
- Kruppel-like factors research
- Immune Response and Inflammation
- Pregnancy-related medical research
- Ion-surface interactions and analysis
- Peroxisome Proliferator-Activated Receptors
University of Cincinnati Medical Center
2019-2025
Cincinnati Children's Hospital Medical Center
2016-2025
China Medical University
2022-2025
Luye Pharma (China)
2024
China Iron and Steel Research Institute Group
2024
Analysis and Testing Centre
2024
Lanzhou University
2023
University of Cincinnati
2009-2022
Purdue University West Lafayette
2020
China Agricultural University
2012-2018
LC-IMS-MS spectra of lipids in mouse decidua tissue.
During implantation, uterine luminal epithelial (LE) cells first interact with the blastocyst trophectoderm. Within 30 hr after initiation of attachment, LE surrounding in implantation chamber (crypt) disappear, allowing trophoblast to make direct physical contact underneath stroma for successful implantation. The mechanism extraction was thought be mediated by apoptosis. Here, we show that are endocytosed adopting nonapoptotic cell-in-cell invasion process (entosis) absence caspase 3...
Embryo homing and implantation occur within a crypt (implantation chamber) at the antimesometrial (AM) pole along uterus. The mechanism by which this is achieved not known. Here, we show that villi-like epithelial projections from main uterine lumen toward AM regularly spaced intervals form crypts for embryo were disrupted in mice with loss or gain of function Wnt5a, both Ror1 Ror2. This disruption Wnt5a-ROR signaling resulted disorderly projections, formation, spacing, impaired...
Unraveling the complexity of lipidome requires development novel approaches for structural characterization lipid species with isomer-level discrimination. Herein, we introduce an online photochemical approach isomer identification through selective derivatization double bonds by reaction singlet oxygen. Lipid hydroperoxide products are generated promptly after laser irradiation. Fragmentation these in a mass spectrometer produces diagnostic fragments revealing C=C locations unreacted...
The incidence of testicular germ cell tumors (TGCTs) has been increasing the past 4 to 6 decades; however, exposures that account for this rise have not identified. Marijuana use also grew during same period, and it established chronic marijuana produces adverse effects on human endocrine reproductive systems. In study, authors tested hypothesis is a risk factor TGCT.A population-based, case-control study 369 men ages 18 44 years who were diagnosed with TGCT from January 1999 through 2006...
Leukemia inhibitory factor (LIF), a downstream target of estrogen, is essential for implantation in mice. LIF function thought to be mediated by its binding receptor (LIFR) and recruitment coreceptor GP130 (glycoprotein 130), this complex then activates signal transducer activator transcription (STAT)1/3. However, the importance LIFR acting via STAT3 remains uncertain, because constitutive inactivation Lifr, Gp130, or Stat3 shows embryonic lethality To address issue, we generated mice with...
Murine small intestinal crypt development is initiated during the first postnatal week. Soon after formation, overall increases in number of crypts occurs through a bifurcating process called fission, which believed to be driven by developmental stem cells (ISCs). Recent evidence suggests that heterogeneous population ISCs exists within adult intestine. Actively cycling are labeled Lgr5, Ascl2 and Olfm4; whereas slowly or quiescent ISC marked Bmi1 mTert. The goal this study was correlate...
Embryo implantation is central to pregnancy success. Our previous understanding limited by studying this phenomenon primarily in two dimensions. Here we employ 3D visualization, revealing that epithelial evaginations form chambers (crypts) consistently arise with preexisting glands, suggesting direct access of glands embryos within the chamber. While lobular domains become more developed, ductal regions continue elongate and progressively stretch following implantation. Using diapausing mice...
Although epidemiologic and experimental evidence strongly implicates chronic inflammation dietary fats as risk factors for cancer, the mechanisms underlying their contribution to carcinogenesis are poorly understood. Here we present genetic demonstrating that deletion of peroxisome proliferator-activated receptor δ (PPARδ) attenuates colonic colitis-associated adenoma formation/growth. Importantly, PPARδ is required dextran sodium sulfate induction proinflammatory mediators, including...
Abstract Scribble (Scrib) is a scaffold protein with multifunctional roles in PCP, tight junction and Hippo signaling. This study shows that Scrib expressed stromal cells around the implantation chamber following implantation. Stromal transform into epithelial-like to form avascular primary decidual zone (PDZ) (crypt). The PDZ creates permeability barrier crypt restricting immune harmful agents from maternal circulation protect embryonic health. mechanism underlying formation not yet known....
Abstract Unraveling the complexity of lipidome requires development novel approaches for structural characterization lipid species with isomer‐level discrimination. Herein, we introduce an online photochemical approach isomer identification through selective derivatization double bonds by reaction singlet oxygen. Lipid hydroperoxide products are generated promptly after laser irradiation. Fragmentation these in a mass spectrometer produces diagnostic fragments revealing C=C locations...
Nanospray desorption electrospray mass spectrometry imaging (nano-DESI MSI) enables quantitative mapping of hundreds molecules in biological samples with minimal sample pretreatment. We have recently developed an integrated microfluidic probe (iMFP) for nano-DESI MSI. Herein, we describe improved design the iMFP high-throughput tissue sections. increased dimensions primary and spray channels optimized voltage solvent flow rate to obtain a stable operation at both low high scan rates. observe...
In most mammals, placentation is critical for fetal development and pregnancy success. Exposure to marijuana during has adverse effects, but whether the placenta a target of cannabinoid/endocannabinoid signaling not known. Using mice as model system, we found that endocannabinoid system present in ectoplacental cone spongiotrophoblast cells. We also observed aberrant confers premature trophoblast stem cell differentiation, defective invasion. These defects are reflected retarded compromised...
A blastocyst will implant only when the uterus becomes receptive. Following attachment, luminal epithelial cells undergo degeneration at site of blastocyst. Although many genes critical for uterine receptivity are primarily regulated by ovarian hormones, Kruppel-like factor 5 (KLF5), a zinc finger-containing transcription factor, is persistently expressed in independently hormones. Loss Klf5 causes female infertility due to defective implantation. Cox2 normally epithelium and stroma but COX2...
Objectives Statins and calcium channel blockers have been proven beneficial toward improvement of endothelial function. The aim this study was to compare the effect combination therapy statin blocker with solo treatment in patients cardiac syndrome X. Methods results Sixty-eight X were divided randomly into three groups: fluvastatin (40 mg/day, n=23), diltiazem (90 n=22), mg/day) n=23). At end 90 days, coronary flow reserve improved groups (fluvastatin-treated group: 23.2%; diltiazem-treated...
Preterm birth (PTB) is a leading worldwide cause of morbidity and mortality in infants. Maternal inflammation induced by microbial infection critical predisposing factor for PTB. However, biological processes associated with competency pathogens, including viruses, to induce PTB or sensitize secondary bacterial infection-driven are unknown. We show that pathogen/pathogen-associated molecular pattern-driven activation type I IFN/IFN receptor (IFNAR) was sufficient prime systemic uterine...
Blastocyst implantation is a complex process requiring coordination of dynamic sequence embryo-uterine interactions. Blood vessels enter the uterus from mesometrium, demarcating into mesometrial (M) and antimesometrial (AM) domains. Implantation occurs along uterine longitudinal axis within specialized chambers (crypts) that originate evaginations directed primary lumen toward AM domain. The morphological orientation crypts in rodent uteri was recognized more than century ago, but mechanism...
Abstract Global proteomic analyses of complex protein samples in nanogram quantities require a fastidious approach to achieve in-depth coverage and quantitative reproducibility. Biological are often severely mass limited can preclude the application more robust bulk sample processing workflows. In this study, we present system that minimizes handling by using online immobilized trypsin digestion solid phase extraction create simple, sensitive, robust, reproducible platform for analysis...