A5024251545- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Nanoplatforms for cancer theranostics
- Cancer Cells and Metastasis
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Immunotherapy and Immune Responses
- Adenosine and Purinergic Signaling
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- TGF-β signaling in diseases
- Cytokine Signaling Pathways and Interactions
- Ferroptosis and cancer prognosis
- Nanoparticle-Based Drug Delivery
- Cancer-related Molecular Pathways
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- Macrophage Migration Inhibitory Factor
- Cancer Mechanisms and Therapy
- MicroRNA in disease regulation
Southern Medical University
2021-2024
Wuhan University
2014-2021
Ministry of Education of the People's Republic of China
2016-2018
Yangzhou University
2015
For decades, poly(ethylene glycol) (PEG) has been widely incorporated into nanoparticles for evading immune clearance and improving the systematic circulation time. However, recent studies have reported a phenomenon known as “accelerated blood (ABC)” where second dose of PEGylated nanomaterials is rapidly cleared when given several days after first dose. Herein, we demonstrate that natural red cell (RBC) membrane superior alternative to PEG. Biomimetic RBC membrane‐coated Fe 3 O 4 (Fe @RBC...
Although anti-PD-1 immunotherapy is widely used to treat melanoma, its efficacy still has be improved. In this work, we present a therapeutic method that combines and starvation therapy achieve better antitumor efficacy. We designed the CMSN-GOx method, in which mesoporous silica nanoparticles (MSN) are loaded with glucose oxidase (GOx) then encapsulate surfaces of cancer cell membranes realize therapy. By functionalizing MSN's biomimetic surfaces, can synthesize escape host immune system...
Abstract Biomimetic cell‐membrane‐camouflaged nanoparticles with desirable features have been widely used for various biomedical applications. However, the current research focuses on single cell membrane coating and using multiple membranes nanoparticle functionalization is still challenging. In this work, platelet (PLT) leukocyte (WBC) are fused, PLT–WBC hybrid coated onto magnetic beads, then their surface modified specific antibodies. The resulting membrane‐coated immunomagnetic beads...
Abstract Cell membrane–based nanosystems with desirable characteristics have been studied extensively for many therapeutic applications. However, current research has focused on single cell membrane, and multifunctional fused membrane materials from different types are still rare. Herein, a platelet–cancer stem (CSC) hybrid membrane‐coated iron oxide magnetic nanoparticle (MN) {[CSC‐P]MN} is presented the first time enhanced photothermal therapy of head neck squamous carcinoma (HNSCC)....
Abstract A major challenge for traditional cancer therapy, including surgical resection, chemoradiotherapy, and immunotherapy, is how to induce tumor cell death leverage the host immune system at same time. Here, a myeloid‐derived suppressor (MDSC) membrane‐coated iron oxide magnetic nanoparticle (MNP@MDSC) overcome this conundrum therapy developed. In study, MNP@MDSC demonstrates its superior performance in evasion, active tumor‐targeting, resonance imaging, photothermal (PTT)‐induced...
Abstract Immunosuppression is common in head and neck squamous cell carcinoma (HNSCC). In previous studies, the TIGIT/CD155 pathway was identified as an immune-checkpoint signaling that contributes to “exhaustion” state of infiltrating T cells. Here, we sought explore clinical significance HNSCC identify therapeutic effect a transgenic mouse model. TIGIT overexpressed on tumor-infiltrating CD8+ CD4+ cells both patients models, correlated with molecules (PD-1, TIM-3, LAG-3). also expressed...
Abstract Cell membrane coating nanotechnology, which endows nanoparticles with unique properties, displays excellent translational potential in cancer diagnosis and therapy. However, the preparation evaluation of these cell membrane‐coated are based on lines cell‐line‐based xenograft mouse models. The feasibility membrane‐camouflaged nanomaterials is tested a preclinical setting. Head neck squamous carcinoma (HNSCC) patient‐derived tumor (PDTC) membranes coated onto gelatin (GNPs) resulting...
The limited clinical response and serious side effect have been challenging in cancer immunotherapy resulting from immunosuppressive tumor microenvironment (TME) inferior drug targeting. Herein, an active targeting TME nanoplatform capable of revising the is designed. Briefly, gold nanorods (GNRs) are covered with silica dioxide (SiO2) then coated manganese (MnO2) to obtain GNRs@SiO2@MnO2 (GSM). Myeloid-derived suppressor cells (MDSCs) membrane further camouflaged on surface GSM...
Dexamethasone (DEX) is the first drug to show life-saving efficacy in patients with severe coronavirus disease 2019 (COVID-19), while DEX associated serious adverse effects. Here, we report an inhaled, Self-immunoregulatory, Extracellular Nanovesicle-based Delivery (iSEND) system by engineering neutrophil nanovesicles cholesterols deliver for enhanced treatment of COVID-19. Relying on surface chemokine and cytokine receptors, iSEND showed improved targeting macrophages neutralized...
Head and neck cancer is one of the most prevalent cancers around world. squamous cell carcinoma (HNSCC) accounts for nearly 90% head cancer. In recent years, significant advances have been made in immunotherapy HNSCC. Although some clinical trials targeting immune checkpoints shown success, molecular mechanism regulation programmed death 1 (PD-1) its ligand (PD-L1) partially understood. an effort to explore effect activation signal transducers activators transcriptions (STAT3) on PD-1/PD-L1,...
Cancer immunotherapy offers a promising approach in cancer treatment. The adenosine A2A receptor (A2AR) could protect cancerous tissues from immune clearance via inhibiting T cells response. To date, the role of A2AR head and neck squamous cell carcinoma (HNSCC) has not been investigated. Here, we sought to explore expression immunotherapeutic value blockade HNSCC.The was evaluated by immunostaining 43 normal mucosae, 48 dysplasia 165 primary HNSCC tissues. assessed vivo genetically defined...
Immunotherapy with immune checkpoint molecule-specific monoclonal antibody have obtained encouraging results from preclinical studies and clinical trials, which promoted us to explore whether this kind of immunotherapy could be applicable head neck squamous cell carcinoma (HNSCC). Lymphocyte activation gene-3 (LAG-3) is an control protein that negatively regulates T cells response. Here, using the human tissue samples, we report these findings LAG-3 overexpressed on tumor-infiltrating...
NLRP3 inflammasome acts as a danger signal sensor that triggers and coordinates the inflammatory response. However, roles of in tumorigenesis development cancer stem cells (CSCs) squamous cell carcinoma head neck (SCCHN) remain ambiguous. In our study, tissue microarrays, ELISA, sphere-forming assay, colony formation assay Western blot analysis were performed to evaluate effect on CSCs human SCCHN specimen, lines, transgenic mouse model. The components inflammasome, namely, NLRP3, ASC,...
Nanotechnology possesses the potential to revolutionize diagnosis and treatment of tumors. The ideal nanoparticles used for in vivo cancer therapy should have long blood circulation times active targeting. Additionally, they be harmless invisible immune system. Here, we developed a biomimetic nanoplatform with above properties therapy. Macrophage membranes were reconstructed into vesicles then coated onto magnetic iron oxide (Fe3O4 NPs). Inherited from Fe3O4 core macrophage membrane shell,...
// Guang-Tao Yu 1 , Lin-Lin Bu Cong-Fa Huang Wen-Feng Zhang 2 Wan-Jun Chen 3 J. Silvio Gutkind Ashok B. Kulkarni 4 Zhi-Jun Sun 1, 2, The State Key Laboratory Breeding Base of Basic Science Stomatology & Oral Biomedicine, Ministry Education, Wuhan University, Wuhan, China Department Maxillofacial-Head Neck Oncology, School and Hospital Stomatology, Pharyngeal Cancer Branch, Cell Developmental Biology, National Institute Dental Craniofacial Research, Institutes Health, Bethesda, MD, USA...
Abstract Cancer stem cells (CSCs) are considered responsible for tumor initiation and chemoresistance. This study was aimed to investigate the possibility of targeting head neck squamous cell carcinoma (HNSCC) by NOTCH1 pathway inhibition explore synergistic effect combining NOTCH with conventional chemotherapy. NOTCH1/HES1 elevation found in human HNSCC, especially tissue post chemotherapy lymph node metastasis, which is correlated CSCs markers. inhibitor DAPT (GSI-IX) significantly reduces...
Herein, we report that genetically programmable fusion cellular vesicles (Fus-CVs) displaying high-affinity SIRPα variants and PD-1 can activate potent antitumor immunity through both innate adaptive immune effectors. Dual-blockade of CD47 PD-L1 with Fus-CVs significantly increases the phagocytosis cancer cells by macrophages, promotes antigen presentation, activates T-cell immunity. Moreover, bispecific targeting design ensures better on tumor cells, but less other which reduces systemic...
Parkinson's disease (PD) is a neurodegenerative disorder characterized by the gradual loss of dopaminergic neurons in substantia nigra and accumulation α-synuclein aggregates called Lewy bodies. Here, nanodecoys were designed from rabies virus polypeptide with 29 amino acid (RVG29)-modified red blood cell membrane (RBCm) to encapsulate curcumin nanocrystals (Cur-NCs), which could effectively protect neurons. The RVG29-RBCm/Cur-NCs escaped reticuloendothelial system (RES) uptake, enabled...
Abstract The suitable microenvironment of bone regeneration is critically important for periodontitis-derived defect repair. Three major challenges in achieving a robust osteogenic reaction are the exist oral inflammation, pathogenic bacteria invasion and unaffluent seed cells. Herein, customizable multifunctional 3D-printing module was designed with glycidyl methacrylate (GMA) modified epsilon-poly-L-lysine (EPLGMA) loading periodontal ligament stem cells (PDLSCs) myeloid-derived...
Immature myeloid cells such as myeloid-derived suppressor (MDSCs) and M2 macrophages play a vital role in the tumor immune escape progression. Cytotoxic T lymphocyte-associated antigen 4 (CTLA4), negative checkpoint, is highly expressed numerous solid tumors. However, precise functions of CTLA4 head neck squamous cell carcinoma (HNSCC) have not yet been elucidated. In this study, we demonstrated that ratio CD8+/CTLA4 can be used potential index with clinical prognostic value for HNSCC. Using...
T‐cell immunoglobulin mucin 3 ( TIM 3) contributes to immune suppression during progression of many cancers, but the precise role in head and neck squamous cell carcinoma HNSCC ) is not clearly understood. In this study, we report that expression was significantly up‐regulated patients with associated lymph node metastasis. Additionally, increased recurrent preradiotherapy or prechemotherapy. We also characterized CD 8 + T cells 11b 33 myeloid‐derived suppressor MDSC s) human , found their...
Monitoring specific immune cells in vivo will provide significant information for improving the therapeutic effect of immunotherapy. Herein, two-color fluorescence molecular imaging an important cell, myeloid-derived suppressor cell (MDSC), was realized by using quantum dot (QD)-based nanoprobes with non-overlap emission second near-infrared window (NIR-II, 1000–1700 nm). NIR-IIa and NIR-IIb QDs were conjugated two MDSC-specific antibodies, respectively, targeted MDSCs together. Due to...
Accumulated evidence indicates that immune cell populations play pivotal roles in the process of tumor initiation, progression, recurrence, metastasis, and escape. Ferroptosis is a form regulating death nexus between metabolism, redox biology, human health. considered as vital important event HNSCC, but underling mechanism remains poorly understood. Our tissue microarray study showed patients with high expression GPX4 were related to poor survival. Moreover, has been negatively associated...