A5023417757- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Neuroinflammation and Neurodegeneration Mechanisms
- Glioma Diagnosis and Treatment
- Spaceflight effects on biology
- Cancer Cells and Metastasis
- SARS-CoV-2 and COVID-19 Research
- Single-cell and spatial transcriptomics
- Breast Cancer Treatment Studies
- Radiation Therapy and Dosimetry
- COVID-19 Clinical Research Studies
- COVID-19 Impact on Reproduction
- Adipokines, Inflammation, and Metabolic Diseases
- Molecular Biology Techniques and Applications
- Advanced Breast Cancer Therapies
- Thermal Regulation in Medicine
- Wnt/β-catenin signaling in development and cancer
- Nuclear Physics and Applications
- Breast Lesions and Carcinomas
Gdańsk Medical University
2021-2024
Cold ischemia time (CIT), the interval between tissue excision and preservation, is a critical preanalytical variable that profoundly impacts gene expression profiles. Variability in CIT can lead to inconsistent transcriptomic results, making study interpretation challenging undermining reproducibility biomedical research. Our aimed evaluate impact of on cancer-related genes, particularly these involved hypoxia, apoptosis, epithelial-to-mesenchymal transition (EMT). We performed RNA...
Abstract The mammary gland undergoes hormonally stimulated cycles of proliferation, lactation, and involution. We hypothesized that these factors increase the mutational burden in glandular tissue may explain high cancer incidence rate general population, recurrent disease. Hence, we investigated DNA sequence variants normal gland, tumor, peripheral blood from 52 reportedly sporadic breast patients. Targeted resequencing 542 cancer-associated genes revealed subclonal somatic pathogenic of:...
Abstract Background The COVID-19 pandemic, which has a prominent social and economic impact worldwide, shows largely unexplained male bias for the severity mortality of disease. Loss chromosome Y (LOY) is risk factor candidate in due to its prior association with many chronic age-related diseases, on immune gene transcription. Methods Publicly available scRNA-seq data PBMC samples derived from patients critically ill were reanalyzed, LOY status was added annotated cells. We further studied...
Abstract Despite surging interest in space travel recent decades, the impacts of prolonged, elevated exposure to galactic cosmic radiation (GCR) on human health remain poorly understood. This form ionizing causes significant changes biological systems including damage DNA structure by altering epigenetic phenotype with emphasis methylation. Building previous work Kennedy et al. (Sci Rep 8(1): 6709. 10.1038/S41598-018-24755-8), we evaluated spatial methylation patterns triggered high-LET ( 56...
Abstract Despite advances in early detection and treatment strategies, breast cancer recurrence mortality remain a significant health issue. Recent insights suggest the prognostic potential of microscopically healthy mammary gland, vicinity lesion. Nonetheless, comprehensive understanding gene expression profiles these tissues their relationship to patient outcomes missing. Furthermore, increasing trend towards breast‐conserving surgery may inadvertently lead retention existing...
The progress in translational cancer research relies on access to well-characterized samples from a representative number of patients and controls. rationale behind our biobanking are explorations post-zygotic pathogenic gene variants, especially non-tumoral tissue, which might predispose cancers. targeted diagnoses carcinomas the breast (via mastectomy or conserving surgery), colon rectum, prostate, urinary bladder cystectomy transurethral resection), exocrine pancreatic carcinoma as well...
ABSTRACT Despite advances in early detection and treatment strategies, breast cancer recurrence mortality remain a significant health issue. Recent insights suggest the prognostic potential of microscopically healthy mammary gland, vicinity lesion. Nonetheless, comprehensive understanding gene expression profiles these tissues their relationship to patient outcomes is still missing. Furthermore, increasing trend towards breast-conserving surgery may inadvertently lead retention existing...
Normal-appearing mammary tissue from breast cancer patients can harbor significant genetic alterations. We analyzed DNA variants in both normal-looking and tumors 77 poor-prognosis patients, 49 without prognosis bias, gland samples 15 non-cancerous individuals. Whole exome sequencing revealed a higher prevalence of pathogenic post-zygotic (29%) affecting genes like AKT1, PIK3CA, PTEN, TBX3, TP53, compared to 12.5% those criteria (p=0.0008578). Prevailing PIK3CA were recurrent across while...
<ns4:p>Glioblastoma (GBM), a deadly brain tumor, is still one of few lasting challenges contemporary oncology. Current therapies fail to significantly improve patient survival due GBM tremendous genetic, transcriptomic, immunological, and sex-dependent heterogeneity. Over the years, clinical differences between males females were characterized. For instance, higher incidence in or distinct responses cancer chemotherapy immunotherapy have been noted. Despite introduction single-cell RNA...
<ns4:p>Glioblastoma (GBM), a deadly brain tumor, is still one of the few lasting challenges contemporary oncology. Current therapies fail to significantly improve patient survival due GBM’s tremendous genetic, transcriptomic, immunological, and sex-dependent heterogeneity. Over years, clinical differences between males females were characterized. For instance, higher incidence GBM in or distinct responses cancer chemotherapy immunotherapy have been noted. However, despite introduction...
Abstract The progress in translational cancer research relies on access to well-characterized samples from a representative number of patients and controls. rationale behind our biobanking are explorations post-zygotic pathogenic gene variants, especially non-tumoral tissue, which might predispose cancers. targeted diagnoses carcinomas the breast (via mastectomy or conserving surgery), colon rectum, prostate, urinary bladder cystectomy transurethral resection), exocrine pancreatic carcinoma...
Abstract Despite surging interest in space travel recent decades, the impacts of prolonged, elevated exposure to galactic cosmic radiation (GCR) on human health remain poorly understood. This form ionizing causes significant changes biological systems including damage DNA structure by altering epigenetic phenotype with emphasis methylation. Building previous work Kennedy et al. (2018), we evaluated spatial methylation patterns triggered high-LET ( 56 Fe, 28 Si) and low-LET (X rays) influence...
Abstract COVID-19 shows an unexplained, strong male bias for severity and mortality. Loss of Y (LOY) in myeloid cells is a risk factor candidate because associations with many age-related diseases its effect on transcription immune genes. We report the highest levels LOY that are crucial development severe phenotype, such as low-density neutrophils, granulocytes, monocytes reaching 46%, 32%, 29%, respectively, from men critical (n=139). sorted subpopulations leukocytes correlated increased...
Abstract The mammary gland undergoes hormonally stimulated cycles of proliferation, lactation and involution. We hypothesized that these factors increase the mutational burden in glandular tissue may explain high cancer incidence rate general population recurrent disease. Hence, we investigated DNA sequence variants normal gland, tumor peripheral blood from 52 reportedly sporadic breast patients, including breast-conserving surgery cases. Targeted resequencing 542 associated genes revealed...