A5101839969- Neonatal Respiratory Health Research
- Neuroscience of respiration and sleep
- Receptor Mechanisms and Signaling
- Congenital Diaphragmatic Hernia Studies
- Natural product bioactivities and synthesis
- Biochemical Analysis and Sensing Techniques
- Pharmacological Effects of Natural Compounds
- Neuropeptides and Animal Physiology
- Regulation of Appetite and Obesity
- Phytochemistry and Bioactive Compounds
- Traditional Chinese Medicine Analysis
- Ion channel regulation and function
- Diet and metabolism studies
- Cancer Treatment and Pharmacology
- Digestive system and related health
- Respiratory Support and Mechanisms
- Phytochemistry and biological activity of medicinal plants
- Cystic Fibrosis Research Advances
- Birth, Development, and Health
- Cancer therapeutics and mechanisms
- Growth Hormone and Insulin-like Growth Factors
- Nitric Oxide and Endothelin Effects
- Ginkgo biloba and Cashew Applications
- Bioactive Compounds and Antitumor Agents
- Cancer, Hypoxia, and Metabolism
Guiyang Medical University
2016-2025
Southern Medical University
2025
Jiangsu Province Hospital
2023
Hunan Cancer Hospital
2023
Central South University
2023
Xiangya Hospital Central South University
2023
SickKids Foundation
2002-2022
Zhejiang University
2019-2021
Beijing University of Chinese Medicine
2020-2021
Affiliated Hospital of Guizhou Medical University
2020
The molecular mechanisms regulating secretion of the orexigenic-glucoregulatory hormone ghrelin remain unclear. Based on qPCR analysis FACS-purified gastric cells, highly expressed and enriched 7TM receptors were comprehensively identified functionally characterized using in vitro, ex vivo methods. Five Gαs-coupled efficiently stimulated secretion: as expected β1-adrenergic, GIP secretin but surprisingly also composite receptor for sensory neuropeptide CGRP melanocortin 4 receptor. A number...
Abstract Human actin-related protein 2/3 complex (Arp2/3), required for actin filament branching, has two ARPC1 component isoforms, with ARPC1B prominently expressed in blood cells. Here we show a child microthrombocytopenia, eosinophilia and inflammatory disease, homozygous frameshift mutation (p.Val91Trpfs*30). Platelet lysates reveal no greatly reduced Arp2/3 complex. Missense mutations are identified an unrelated patient similar symptoms deficiency. ARPC1B-deficient platelets...
Melanin-concentrating hormone (MCH) is a 19-aa cyclic neuropeptide originally isolated from chum salmon pituitaries. Besides its effects on the aggregation of melanophores in fish several lines evidence suggest that mammals MCH functions as regulator energy homeostasis. Recently, groups reported identification an orphan G protein-coupled receptor for (MCH-1R). We hereby report second human termed MCH-2R, which shares about 38% amino acid identity with MCH-1R. MCH-2R displayed high-affinity...
Background & AimsA proportion of infants and young children with inflammatory bowel diseases (IBDs) have subtypes associated a single gene variant (monogenic IBD). We aimed to determine the prevalence monogenic disease in cohort pediatric patients IBD.MethodsWe performed whole-exome sequencing analyses blood samples from an unselected 1005 IBD, aged 0–18 years (median age at diagnosis, 11.96 years) center Canada their family members (2305 total). Variants believed cause IBD were validated...
Pulmonary neuroendocrine cells (PNEC) produce amine (serotonin, 5-HT) and peptides (e.g., bombesin, calcitonin) with growth factor-like properties are thought to play an important role in lung development. Because physical forces essential for development, we investigated the effects of mechanical strain on 5-HT release PNEC freshly isolated from rabbit fetal PNEC-related tumor H727 cell line. Cultures exposed sinusoidal cyclic stretch showed a significant inhibitable gadolinium chloride (10...
We investigated the development of innervation pulmonary neuroendocrine cell (PNEC) system composed single cells and organoid clusters, neuroepithelial bodies (NEB) in rabbit fetal neonatal lungs. To visualize nerve fibers their contacts with PNECs/NEBs, we used confocal microscopy multilabel immunohistochemistry (IHC) pan-neural marker, synaptic vesicle protein 2 (SV2), serotonin (5-HT) as markers for smooth muscle actin or cytokeratin to identify airway landmarks. The numbers distribution...
Pulmonary neuroendocrine cells (PNEC), including neuroepithelial bodies (NEB), are amine- and peptide (for example, bombesin)–producing that function as hypoxia/hypercapnia-sensitive chemoreceptors could be involved in the pathophysiology of sudden infant death syndrome (SIDS). We assessed morphometrically frequency size PNEC/NEB lungs infants who died SIDS ( n = 21) compared them to an equal number age-matched control accidental or homicide, with all cases obtained from San Diego SIDS/SUDC...
ABSTRACT Gerbera piloselloides Herba (GP) is a traditional herb commonly used for asthma in China. Our previous studies have identified key group of active components—arbutin, marmesin, and luteolin—that demonstrate significant efficacy treatment, showing promising therapeutic potential. Although we previously investigated the comparative pharmacokinetics (only absorption process) 11 main components GP both control asthmatic mice, these 3 compounds, including absorption, metabolism,...
The amine- and peptide-producing pulmonary neuroendocrine cells (PNEC) are widely distributed within the airway mucosa of mammalian lung as solitary innervated clusters, neuroepithelial bodies (NEB), which function O2 sensors. These express Cftr hence could play a role in pathophysiology cystic fibrosis (CF) disease. We performed confocal microscopy morphometric analysis on sections from Cftr-/- (null), Cftr+/+, Cftr+/- (control) mice at developmental stages E20, P5, P9, P30 to determine...
Polygonum orientale L. (PO) represents significant bioactivities in treating myocardial ischemia (MI); however, its underlying mechanisms remain unclear. This study aims to elucidate PO's potential MI using an integrated approach that combines UHPLC-Q-Exactive Orbitrap HRMS, network pharmacology, and RNA-sequencing (RNA-seq). Initially, the chemical constituents of PO were identified HRMS. Subsequently, molecular docking, RNA-seq employed screen active components targeting predict their...
Alzheimer's disease (AD) is a neurodegenerative (NDD) characterized by cognitive decline and behavioral disorders. In recent years, the number of patients has been on rise population becoming increasingly younger, which attracted widespread public attention. At present, research intervention dietary patterns in AD still its early stages, but many animal human experiments have shown that Mediterranean diet (MD) ketogenic (KD) can improve pathological physiological changes patients, thereby...
Pulmonary neuroendocrine cells (PNECs) are the first cell type to differentiate within primitive airway epithelium, suggesting a possible role in lung development. The differentiation of PNECs fetal is governed by proneural genes such as mammalian homolog achaete-scute complex (Mash-1) and related transcription factor, hairy enhancer split1 (Hes-1). We examined expression Mash-1 downstream factor Prox-1 developing mouse wild-type respective knockout models. During early stages (embryonic day...