Abstract Estrogen receptor α (ERα) plays a crucial role in regulating glucose and energy homeostasis during type 2 diabetes mellitus (T2DM). However, the underlying mechanisms remain incompletely understood. Here we find ligand-independent effect of ERα on regulation homeostasis. Deficiency liver impairs male, female, ovariectomized (OVX) female mice. Mechanistic studies reveal that promotes hepatic insulin sensitivity by suppressing ubiquitination-induced IRS1 degradation. The 1-280 domain...

Hypoxic-ischemic brain damage (HIBD) is a major cause of fatality and morbidity in neonates. However, current treatment approaches to alleviate HIBD are not effective. Various studies have highlighted the role microRNAs (miRNAs) various biological functions multiple diseases. This study investigated miR-339-5p progression. Neonatal mouse model was induced by ligation right common carotid artery. Neuronal cell exposed oxygen-glucose deprivation (OGD) also established. The expression tissues...

Background: The occurrence of adverse events after immunochemotherapy in patients with diffuse large B-cell lymphoma (DLBCL) frequently affects the course chemotherapy, leading to a further decline quality life and survival. Objectives: primary objective this study was investigate association between Chinese visceral adiposity index (CVAI), lipid accumulation product (LAP) skeletal muscle mass (SMI) at initial diagnosis risk haematological toxicity following DLBCL. Design: Retrospective,...

Bromodomain-containing protein 4 (BRD4) is overexpressed in thyroid carcinoma, represents as an important therapeutic target.ARV-825 a novel cereblon-based PROTAC (Proteolysis Targeting Chsimera) compound.It can induce fast and sustained BRD4 degradation.Its potential effect human carcinoma cells was studied here.In TPC-1 primary cells, ARV-825 potently inhibited cell viability, proliferation migration.Furthermore, induced robust apoptosis activation the cells.ARV-825 degradation...

This study was aimed at identifying the role of zinc finger protein 143 (ZNF143) in gastric cancer (GC) progression. The impact ZNF143 on proliferation ability and apoptosis GC cells detected. expression related targeted genes determined using Western blot analysis. reactive oxygen species (ROS) level examined ROS generation assay. vivo tumor xenograft ectopic overexpression promoted cells, while its knockdown reduced effect vitro. downregulation facilitated cell apoptosis. decreased...

High-risk human papillomavirus (HPV) infection play an important role in the development of lung cancer. Our previously study showed that E6 and E7 HPV16 upregulated expression GLUT1 cancer cells. However, whether they can promote glucose uptake by underlying molecular mechanism has not been identified. It reported thioredoxin interacting protein (TXNIP) regulates both its uptake. We speculate high risk may be closely related to TXNIP expression. Therefore, we associate with explore...

Chronic infection of HPV16 E6/E7 is frequently associated with lung cancers, especially in non-smokers and Asians.In our previous studies, we found that up-regulated HIF-1α at protein level further GLUT1 both mRNA levels well-established cancer cell lines.In one mechanism study, the results demonstrated expression through HPV-LKB1-HIF-1α-GLUT1 axis.However, there are multiple pathways involved regulation expression.In current using double directional genetic manipulation lines, showed E6 E7...

HPV16 E6/E7 proteins are the main oncogenes and only long-term persistent infection causes lung cancer. Our previous studies have shown that protein up-regulates expression of GLUT1 in cancer cells. However, whether E6 E7 can promote glucose uptake its molecular mechanism unclear.The regulatory relationships or E7, miR-451, CAB39, PI3K/AKT, were detected by double directional genetic manipulations cell lines. Immunofluorescence flow cytometry used to detect effect CAB39 on promoting...

Delta(24(28))-Sterols are end products of a mono C-methylation pathway catalyzed by the native Delta(24(25))- to Delta(24(28))-sterol methyl transferase (SMT) enzyme from Saccharomyces cerevisiae. Using Tyr(81) Phe mutant SMT S. cerevisiae, generated site-directed mutagenesis highly conserved residue in sterol binding site, we found that several and Delta(24(28))-sterols, which not substrates for protein, were mono- bis-C24-alkylated side chains. The protein behaved similarly chromatography...

To explore the role of HOTAIR in pathogenesis adrenocortical carcinoma (ACC) and its underlying mechanism.Differentially expressed lncRNA (HOTAIR) ACC was screened out from GEO database. The survival analysis ROC curve were performed according to expressions patients. correlation between expression clinical information patients analyzed by chi-square test. univariate multivariate COX regression carried analyze relationship expression, disease-free (DFS) overall (OS) We then detected 77...

Objective The correlation between surrogate insulin resistance (IR) indices and sarcopenic obesity (SO) remains uncertain. This study aimed to assess the association six IR surrogates—triglyceride-glucose (TyG), TyG-waist circumference (TyG-WC), TyG-waist-to-height ratio (TyG-WHtR), triglyceride-to-high-density lipoprotein-cholesterol (TG/HDL), metabolic score for (METS-IR), Chinese visceral adiposity index (CVAI)—and SO risk in a middle-aged older population China. Methods employed...

Liver kinase B1 (LKB1) is a critical tumor suppressor that frequently mutated in human cancers. LKB1 has serine/threonine protein activity, which regulates gene expression by phosphorylation of Yes-Associated (YAP). The phosphorylation-dependent YAP shuttling critically important intracellular mechanism the Hippo pathway. In our previous study, we found amplification hTERC was significant higher bronchial brushing cells patients with lung cancer, however, underlying molecular not clear. this...

Abstract Expressed sequence tags (ESTs) are widely used in gene survey research these years. The EST Pipeline System, software developed by Hangzhou Genomics Institute (HGI), can automatically analyze different scalar sequences suitable methods. All the analysis reports, including those of vector masking, assembly, annotation, Gene Ontology classification, and some other analyses, be browsed searched as well downloaded Excel format from web interface, saving efforts routine data processing...

The purpose of this study is to extract potential signatures involved in necrotizing enterocolitis (NEC). data set GSE68054 was acquired from Gene Expression Omnibus repertory, and differentially expressed genes were screened between NEC control group. After that, the target prediction analysis functional enrichment carried out. Moreover, protein-protein interaction (PPI) analyses top 20 key PPI network undertaken. In addition, drug-gene also out screen for treatment. Totally, there 16...

In computer's word, the basic idea of DNA computing is using processing power organic molecular information instead digital switch components. recent years, has become one important researches which solve complex problems. this paper, in comparison with analysis development computing, we introduce working principle and mathematical model, three branches calculation model. At last, discuss travel salesman problem as an example, put forward coding method sequence.

The occurrence of hypoxia-ischemia (HI) in the developing brain is closely associated with neuronal injury and even death. However, underlying molecular mechanism not fully understood. This study was designed to investigate phosphatase tensin homolog (PTEN) nuclear translocation its possible role rat cortical damage following oxygen-glucose deprivation (OGD) vitro. An vitro OGD model established using primary neurons dissected from newborn Sprague-Dawley rats mimic HI conditions. PTENK13R...

Abstract Domestic pig (Sus scrofa domestica) is one of the most important mammals to humans. Alternative splicing a cellular mechanism in eukaryotes that greatly increases diversity gene products. Expression sequence tags (ESTs) have been widely used for discovery, expression profile analysis, and alternative detection. In this study, total 712,905 ESTs extracted from 101 different non-normalized EST libraries domestic were analyzed. These cover nervous system, digestive immune meat...

BACKGROUND:This study aimed to investigate the effects of paeonol-platinum(II) (PL-Pt[II]) complex on SW1736 human anaplastic thyroid carcinoma cell line and BHP7-13 papillary in vitro mouse tumor xenografts vivo. MATERIAL AND METHODS:The cytotoxic PL-Pt(II) cells was measured using MTT assay. Western blot expression levels cyclins, apoptotic proteins, signaling proteins. DNA content apoptosis were detected by flow cytometry. established mice followed treatment with complex....