A5100402449- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Heat shock proteins research
- Enzyme Structure and Function
- Bioactive Compounds and Antitumor Agents
- Computational Drug Discovery Methods
- Ion Channels and Receptors
- Protein Structure and Dynamics
- Selenium in Biological Systems
- Synthesis and biological activity
- Animal Nutrition and Physiology
- Epigenetics and DNA Methylation
- Clinical Nutrition and Gastroenterology
- SARS-CoV-2 and COVID-19 Research
- Cancer-related gene regulation
- Ion channel regulation and function
- Plant Stress Responses and Tolerance
- Protein Degradation and Inhibitors
- Pharmacological Effects of Natural Compounds
- Infant Nutrition and Health
- Bioinformatics and Genomic Networks
- RNA and protein synthesis mechanisms
- Biochemical Analysis and Sensing Techniques
- Birth, Development, and Health
- Histone Deacetylase Inhibitors Research
Gannan Medical University
2018-2025
Chinese Academy of Sciences
2014-2025
Shanghai Institute of Materia Medica
2007-2025
Ministry of Education of the People's Republic of China
2005-2024
Northwest University
2018-2024
Sichuan Agricultural University
2014-2024
Jimei University
2024
Third Xiangya Hospital
2024
Central South University
2023-2024
Sino Biopharmaceutical (China)
2024
Changes in mucosal defense have been implicated as important factors affecting infectious complications critically ill patients. To study the effects of nutrient administration on gut-associated lymphatic tissue (GALT), ICR mice were randomized to receive chow plus intravenous saline, feeding a total parenteral nutrition (TPN) solution, or enteral same TPN solution. In second series experiments, more complex diet (Nutren) was compared with and TPN. After 5 days experimental diets,...
Au nanoparticles modified with electroactive Prussian blue (PB) were for the first time synthesized by a simple chemical method. Transmission electronic microscopy showed that average size of shell/Au core hybrid composite (PB@Au) was about 50 nm, and Fourier transform IR, UV−vis spectra, cyclic voltammetry confirmed existence PB on surface nanoparticles. Using LbL technique, multilayer thin films PB@Au prepared alternate adsorption oppositely charged linear polyelectrolyte poly(allylamine...
Objective The authors examine the effect of route and type nutrition on an established upper respiratory tract immunity investigate potential mechanisms for increased pneumonia rates in critically injured patients fed parenterally. Summary Background Data primary immunologic defense against many mucosal infections is IgA. Prior work shows that mice total parenteral (TPN) solutions either intravenously or intragastrically had small intestinal gut-associated lymphoid tissue (GALT) atrophy...
Abstract Members of the transient receptor potential (TRP) ion channels conduct cations into cells. They mediate functions ranging from neuronally mediated hot and cold sensation to intracellular organellar primary ciliary signaling. Here we report a cryo-electron microscopy (cryo-EM) structure TRPC4 in its unliganded (apo) state an overall resolution 3.3 Å. The reveals unique architecture with long pore loop stabilized by disulfide bond. Beyond shared tetrameric six-transmembrane fold,...
Significance Ion channels are pore-forming proteins spanning biological membranes. Transient receptor potential ion a subclass of channel proteins, characterized by nonselective permeability to cations such as sodium, calcium, magnesium, and zinc, little voltage sensitivity; their gating is still an area active investigation. TRPM6 TRPM7 ubiquitously expressed with prominent roles in early embryonic development. Uniquely, these also include kinase domain. The functions correlated proteolytic...
Recognizing acetyllysine of histone is a vital process epigenetic regulation that mediated by protein module called bromodomain. To contribute novel scaffolds for developing into bromodomain inhibitors, we utilize fragment-based drug discovery approach. By successively applying docking and X-ray crystallography, were able to identify 9 fragment hits from diffracting more than 60 crystals. In the present work, described four them carried out integrated lead optimization 8, which bears...
Although magnesium (Mg2+) is the most abundant divalent cation in plant cells, little known about mechanism of Mg2+ uptake by roots. Here, we report a key function Magnesium Transport6 (MGT6)/Mitochondrial RNA Splicing2-4 and low-Mg2+ tolerance Arabidopsis thaliana. MGT6 expressed mainly aerial tissues when levels are high soil or growth medium. Its expression highly induced roots during deficiency, suggesting role for response to status Silencing transgenic plants interference (RNAi)...
Research reveals the structure of TRPC5 ion channel, a potential drug target kidney disease, depression and anxiety.
Significance Ion channels are proteins that mediate the flow of ions across cell membranes. Human genetic mutations one type ion channel, called hTRPM4, underlie a form progressive familial heart block. Its distribution among many tissues, however, suggests its functions broad. We have solved atomic structure hTRPM4 to an overall resolution 3.7 Å. The channel is composed four identical subunits surrounding central pore. show path Na + through and point out aspects channel’s internal...
Magnesium (Mg) is a relatively mobile element that remobilized in plants under Mg-limited conditions through transport from old to young tissues. However, the physiological and molecular mechanisms underlying Mg remobilization remain poorly understood. In this study, we investigated rice (Oryza sativa) as facilitated dechelatase gene involved chlorophyll degradation, STAY-GREEN (OsSGR). We first observed mid-aged leaves of are more susceptible deficiency. Expression OsSGR was specifically...
Weanling pigs, with immature immune system and physiological function, usually experience post-weaning diarrhea. This study determined the effects of dietary Clostridium butyricum supplementation on growth performance, diarrhea, immunity weaned pigs challenged lipopolysaccharide (LPS).In Experiment (Exp.) 1, 144 piglets were at 21 d randomly assigned to six groups, replicates per group four replicate, receiving a control diet (CON) or supplemented antibiotics (AB) C. (CB) (0.1%, 0.2%, 0.4%,...
We investigated the effects of dietary supplementation with Bacillus subtilis PB6 (B. PB6) during late gestation and lactation on sow reproductive performance, antioxidant indices, gut microbiota. A total 32 healthy Landrace × Yorkshire sows d 90 were randomly assigned to 2 groups, 16 replicates per group, receiving basal diet (CON) or + 0.2% B. PB6, containing 4.0 108 CFU/kg feed (BS). The litter sizes (total born) numbers piglets born alive larger in BS group (P < 0.01), whereas weights...
Compared with normal cells, tumour cells contain elevated levels of reactive oxygen species (ROS). Increased the antioxidant protein NAD(P)H:quinone oxidoreductase 1 (NQO1) and phosphorylated signal transducer activator transcription 3 (pSTAT3) correlate negatively survival patients pancreatic cancer. Napabucasin is an investigational, orally administered ROS generator bioactivated by NQO1.In open-label, phase CanStem111P study (NCT02993731), adults previously untreated metastatic...
Objective To examine the levels of a Th1 IgA-inhibiting cytokine (interferon γ) and Th2 IgA-stimulating cytokines (interleukin [IL]-4, IL-5, IL-6, IL-10) within intestine animals manipulated with enteral or parenteral nutrition, to correlate these alterations intestinal IgA levels. Summary Background Data Enteral feeding significantly reduces incidence pneumonia in critically injured patients compared intravenous total nutrition (IV TPN) no nutritional support. Experimentally, complex diets...
In addition to its role in absorbing nutrients, the intestinal mucosa provides an important barrier against toxins and bacteria bowel lumen. This study evaluated changes rat jejunal permeability histology after total parenteral nutrition (TPN) or TPN supplemented with glutamine. Lactulose mannitol were used measure permeability, fixed stained histologic specimens mucosal dimensions. After insertion of central venous catheters, 18 male rats randomly divided into three groups: CHOW, saline...
The activation of molecular chaperone heat-shock protein 90 (Hsp90) is dependent on ATP binding and hydrolysis, which occurs in the N-terminal domains protein. Here, we have determined three crystal structures domain human Hsp90 native complex with analog, providing a clear view catalytic mechanism hydrolysis by Hsp90. Additionally, leads to be an intermediate state that could used partially explain why isolated has very weak hydrolytic activity.
CR Climate Research Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 56:231-244 (2013) - DOI: https://doi.org/10.3354/cr01156 Northern Hemisphere temperature reconstruction during last millennium using multiple annual proxies Feng Shi1,2, Bao Yang2,*, Aurèlien Mairesse3, Lucien von Gunten4, Jianping Li1, Achim Bräuning5, Fengmei Yang6, Xia Xiao1 1State Key Laboratory of Numerical Modeling for...
// Junming Chen 1, 2 , Yongmei Xia 2, 3 Xiaochen Sui Qingrui Peng Tongtong Zhang Jian Li 4 and Jue 1 State Key Laboratory of Food Science Technology, Jiangnan University, Wuxi, Jiangsu 214122, China Synthetic Biological Colloids (Ministry Education), School Chemical Materials Engineering, International Joint on Safety, Nuclear Medicine Ministry Health, Institute Medicine, 214063, Correspondence to: Xia, email: ymxia@jiangnan.edu.cn Keywords: steviol; steviol glycoside; cancer;...