Background: Cases of excessive neutrophil counts in the blood severe coronavirus disease (COVID-19) patients have drawn significant attention. Neutrophil infiltration was also noted on pathological findings from autopsy. It is urgent to clarify pathogenesis neutrophils leading pneumonia COVID-19. Methods: A retrospective analysis performed 55 COVID-19 classified as mild (n = 22), moderate 25), and 8) according Guidelines released by National Health Commission China. Trends relating leukocyte...

Abstract Background Ketone body β-hydroxybutyrate (BHB) has received more and attentions, because it possesses a lot of beneficial, life-preserving effects in the fields clinical science medicine. However, role BHB intestinal inflammation not yet been investigated. Methods Colonic mucosa inflammatory bowel disease (IBD) patients healthy controls were collected for evaluation level. Besides, therapeutic effect exogenous murine model acute dextran sulfate sodium (DSS)-induced colitis assessed...

Interleukin (IL)-1β, as a key biomarker and mediator of vascular calcification in patients with end-stage renal disease (ESRD), may be involved the process premature senescence smooth muscle cells (VSMCs). This work sought to investigate whether IL-1β-induced contributes osteoblastic transition VSMCs.Eighty-eight ESRD (aged 25-81 years), 11 healthy individuals, 15 cases lesion-free distal radial arteries from dialysis angiostomy were collected this study. Immunohistochemical analysis was...

Abstract Sepsis is a life-threatening organ dysfunction caused by dysfunctional host response to infection. Neutrophils play protective role releasing antibacterial proteins or phagocytizing bacteria. However, excess neutrophils can induce tissue damage. Recently, novel intercellular communication pathway involving extracellular vesicles (EVs) has garnered considerable attention. whether EVs secreted macrophages mediate neutrophil recruitment infected sites yet be studied. In this study, we...

Abstract Myocardial dysfunction is a significant complication of sepsis that associated with elevated mortality rates. Transmembrane BAX inhibitor motif containing 1 (TMBIM1), stress‐responsive protein, has garnered interest in the field cardiovascular disease for its cardioprotective properties. Nevertheless, role TMBIM1 on sepsis‐induced cardiac (SICD) remains unknown. Here, our findings revealed elevation expression within myocardium following endotoxin challenge and further demonstrate...

This study aimed to determine the impact of a ketogenic diet (KD) on neurobehavioral development when used treat children with intractable epilepsy, confirming efficacy KD, as well correlation between early electroencephalography (EEG) changes in stage treatment efficacy.We enrolled 42 who were starting for epilepsy classic KD protocol. The total quotient quotients adaptability, gross motor movements, fine language, and individual-social interaction Gesell developmental scales assessed...

Patients who suffer from sepsis typically experience acute lung injury (ALI). Extracellular vesicles (EVs) contain miRNAs, which are potentially involved in ALI. However, strategies to screen more effective EV-miRNAs as therapeutic targets yet be elucidated. In this study, functional were identified based on multiomics analysis of single-cell RNA sequencing targeted organs and serum EV (sEV) miRNA profiles patients with sepsis. The proportions neutrophils macrophages increased significantly...

Neutrophilic inflammation occurs during asthma exacerbation, and especially, in patients with steroid-refractory asthma, but the underlying mechanisms are poorly understood. Recently, a significant accumulation of neutrophil extracellular traps (NETs) airways neutrophilic has been documented, suggesting that NETs may play an important role pathogenesis. In this study, we firstly demonstrated could induce human airway epithelial cell damage vitro. mouse asthmatic model neutrophil-dominated...

Scutellaria baicalensis Georgi (SBG), a widely used traditional Chinese medicine, exhibits anti-inflammatory and antioxidant properties. Wogonin is one of the primary bioactive components SBG. Acetaminophen (APAP)-induced liver injury (AILI) represents prevalent form drug-induced damage primarily driven by inflammatory responses oxidative stress.

Efficient neutrophil migration to infection sites plays a vital role in the body's defense against bacterial infections and natural immune responses. Neutrophils have short lifespan cannot be mass-cultured vitro. Therefore, developing more stable artificial neutrophils (AN) controllable manner has become research focus. However, existing AN lack chemotaxis, which is ability migrate toward high-signal-concentration positions dynamic blood- flow environment. Supplying with chemotaxis key...

Sepsis is a serious condition with high mortality rate worldwide. Granisetron an anti-nausea drug for patients undergoing chemotherapy. Here we aimed to identify the novel effect of granisetron on sepsis-induced acute lung injury (ALI). Our results showed that mice treated displayed less severe damage than controls. administration reduced pulmonary neutrophil recruitment after CLP. Moreover, expressions Cxcl1 and Cxcl2 were diminished in presence THP-1 macrophages lipopolysaccharide...

Excessive vascular remodeling has been shown in hypertensive patients. In experimental models of injury, such as angiotensin II (Ang II) challenged mice, toll like receptor 2 (TLR2) initiates inflammatory responses. More recently, studies have reported atypical endothelial to mesenchymal transition (EndMT) injuries and conditions. Here, we aimed investigate whether TLR2 mediates Ang II-induced inflammation EndMT. a mouse model hypertension, show that aortas exhibit increased medial...

Sepsis‐induced acute lung injury (ALI) is a life‐threatening disorder with intricate pathogenesis. Macrophage pyroptosis reportedly plays vital role in ALI. Although it has been established that angiotensin receptor blockers (ARBs) can reduce sepsis‐induced organ injury, the efficacy of sacubitril/valsartan (SV) for sepsis largely understudied. Here, we aimed to investigate SV Caecal ligation and puncture (CLP) were used induce polymicrobial related The therapeutic effects CLP mice...

Background: Cases of excessive neutrophil counts in the blood severe coronavirus disease (COVID-19) patients have drawn significant attention. Neutrophil infiltration was also noted on pathological findings from autopsy. It is urgent to clarify pathogenesis neutrophils leading pneumonia COVID-19. Methods: A retrospective analysis performed 55 COVID-19 classified as mild (n = 22), moderate 25), and 8) according Guidelines released by National Health Commission China. Trends relating leukocyte...

The aim of this study was to evaluate the efficacy ketogenic diet (KD) for infantile spasms (IS) in patients with and without different causative genetic mutations.We retrospectively evaluated data 119 infants IS who underwent whole-exome sequencing (WES) before KD treatment. analyzed at 16th week after initiation. Patients showing ≥ 50% seizure reduction from baseline and/or disappeared hypsarrhythmia were considered as responders. Chi-squared tests or two-sided Fisher's exact performed...

Background: Ketosis is one of the most frequent and costly metabolic disorders in high-producing dairy cows, negatively associated with health reproductive performance bovine. mainly caused by accumulation ketone body β-hydroxybutyric acid its diagnosis based on β-hydroxybutyrate (βHB) concentration blood. Methods: In this study, we investigated effects βHB bovine oocyte maturation subclinical (1.2 mM) clinical (3.6 mM). Results: The results showed disrupted development capacity. Further...

The purpose of this study was to evaluate the antioxidant nature tea polyphenol on S180 cells induced liver cancer in mice. In present study, hepatocellular carcinoma by tumor transplantation situ. antitumor activity has been determined vivo mice after treatment drug (50, 100, 150 mg/kg body weight) gavage for 20 days. Results showed that a significant increase serum aspartate transaminase (AST), alkaline phosphatase (ALP), alanine aminotransfere (ALT), malondialdehyde (MDA) level, decrease...

Enzyme-linked immunosorbent assay(ELISA) and metabolomics were used to analyze compare two animal models of heart-kidney insomnia, in order explore a more ideal model preliminarily the essence insomnia. Based on clinical symptoms disease characteristics insomnia was reproduced through intraperitoneal injection with p-chlorophenylalanine(PCPA) multi-factor interaction. The disease-syndrome combination evaluated by behavioral observation, ELISA metabolomics. Wistar rats randomly divided into...

Ethnopharmacological relevance: Scutellaria baicalensis Georgi (SBG) is a traditional Chinese medicine widely used for its anti-inflammatory and antioxidant properties. Wogonin one of the primary bioactive components it. Acetaminophen(APAP)-induced liver injury(AILI) represents most prevalent forms drug-induced damage, primarily driven by inflammatory responses oxidative stress.Aim study: To investigate specific effects underlying mechanisms on AILI.Materials methods: C57BL/6 J mice were...