A5044240174- Neuroscience and Neural Engineering
- Retinal Development and Disorders
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Bone Tissue Engineering Materials
- Bone health and treatments
- RNA Interference and Gene Delivery
- Liver Disease Diagnosis and Treatment
- Cancer-related gene regulation
- Diet, Metabolism, and Disease
- 3D Printing in Biomedical Research
- Alcohol Consumption and Health Effects
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
- Cellular Mechanics and Interactions
- PI3K/AKT/mTOR signaling in cancer
- Pluripotent Stem Cells Research
- Neuroendocrine Tumor Research Advances
- vaccines and immunoinformatics approaches
- Antimicrobial Peptides and Activities
- Periodontal Regeneration and Treatments
- Endoplasmic Reticulum Stress and Disease
- Orthopaedic implants and arthroplasty
- Tissue Engineering and Regenerative Medicine
- Immune Response and Inflammation
University of Iowa
2019-2024
University of Missouri
2016-2024
University of Central Florida
2016-2019
Icahn School of Medicine at Mount Sinai
2018
Dana-Farber Brigham Cancer Center
2008
Brigham and Women's Hospital
2008
Harvard University
2008
Dana-Farber Cancer Institute
2008
Massachusetts General Hospital
2008
The PI3K/Akt/mTOR pathway is activated in the majority of pancreatic cancers, and inhibition this has antitumor effects preclinical studies. We performed a multi-institutional, single-arm, phase II study RAD001(everolimus), an oral inhibitor mTOR, patients who experienced treatment failure on first-line therapy with gemcitabine.Thirty-three gemcitabine-refractory, metastatic cancer were treated continuously RAD001 at 10 mg daily. Prior fluorouracil perioperative setting was allowed. Patients...
Vaccines are one of the best health care advances ever developed, having led to eradication smallpox and near polio diphtheria. While tremendously successful, traditional vaccines (i.e., whole-killed or live-attenuated) have been associated with some undesirable side effects, including everything from mild injection site inflammation autoimmune disease Guillain–Barré syndrome. This has recent research focus on developing subunit protein, peptide, DNA vaccines) since they inherently safer...
Due to the continually increasing clinical need heal large bone defects, synthetic graft substitutes have become ever more necessary with calcium phosphates (CaP) widely used due their similarity mineral component of bone. In this research, different concentrations ions (Ca2+), phosphate (Pi), or combination were provided mesenchymal stem cells (MSCs) evaluate influence on proliferation and differentiation. The results suggest that 1–16 mM Ca2+ 1–8 Pi is osteoinductive, but not cytotoxic....
In addition to genetic mutations, epigenetic revision plays a major role in the development and progression of cancer; specifically, inappropriate DNA methylation or demethylation CpG residues may alter expression genes that promote tumorigenesis. We hypothesize repair, specifically repair double strand breaks (DSB) by Non-Homologous End Joining (NHEJ) play this process. Using GFP reporter system inserted into genome HeLa cells, we are able induce targeted damage enables after successfully...
Loss of photoreceptor cells is a primary feature inherited retinal degenerative disorders including age-related macular degeneration and retinitis pigmentosa. To restore vision in affected patients, cell replacement will be required. The ideal donor for this application are induced pluripotent stem (iPSCs) because they can derived from transplanted into the same patient obviating need long-term immunosuppression. A major limitation therapy loss associated with simple methods delivery such as...
Engineered scaffolds are commonly used to assist in cellular transplantations, providing crucial support and specific architecture for a variety of tissue engineering applications. Photopolymerization as fabrication technique cell enables precise spatial temporal control properties structure. One simple achieve two-dimensional structure is the use patterned photomask, which results regionally selective photo-cross-linking. However, relationships between photopolymerization parameters like...
Purpose: Widely used approaches for retinal disease modeling and in vitro therapeutic testing can be augmented by using tissue-engineered scaffolds with a precise 3-dimensional structure. However, the materials currently these are poorly matched to biochemical mechanical properties of vivo retina. Here, we create biopolymer-based structure that is amenable tissue engineering modeling. Methods: Optimal two-photon polymerization (TPP) settings, including laser power scanning speed, identified...
The head and neck squamous cell carcinoma (HNSCC) represents one of the most common cancers in humans. Close to 600,000 new diagnoses are made every year worldwide over half diagnosed patients will not survive. In view this low survival rate, development novel cell-based assays for HNSCC allow more mechanistic approaches specific diagnostics each individual patient. provide informative data predicting cellular processes treated patient, which effect would improve patient follow up. More...
Abstract The gut microbiome (GM) represents a large and very complex ecosystem of different microorganisms. There is an extensive interest in the potential role GM diseases including cancer, diabetes, cardiovascular diseases, aging. changes over lifespan strongly associated with various age-related diseases. Ames dwarf (df/df) mice are characterized by extended life- healthspan, although these protected from many their has not been studied. To determine microbiota on longevity animal models,...
Vaccines are an indispensable public health measure that have enabled the eradication, near elimination, and prevention of a variety pathogens. As research continues our understanding immunization strategies develops, subunit vaccines emerged as exciting alternatives to existing whole vaccine approaches. Unfortunately, often possess weak antigenicity, requiring delivery devices adjuvant supplementation improve their utility. Peptide amphiphile micelles recently been shown function both...
Current treatments for retinal degenerative diseases are limited and cell replacement therapies, in tandem with a supportive biomaterial scaffold, serve as promising emerging option. However, the development vitro testing of these therapies require large quantities human progenitor cells (RPCs) to thoroughly assess impact material properties, culture conditions, surgical parameters on survival fate refine optimize this approach. Although induced pluripotent stem (iPSCs) an ideal source RPC...
Abstract Double strand breaks (DSBs) in DNA are repaired throughout the cell cycle and non-dividing cells by error-prone process of non-homologous end joining (NHEJ). In order to evaluate expression a gene following NHEJ repair, we use GFP reporter system allow for targeted induction DSB genome Hela cells. Prior DSB, is transcribed but inactive due an extra exon interrupting sequence, however that successfully repair express WT can be detected fluorescent based assays. Following two...