A5070767586- Zebrafish Biomedical Research Applications
- Toxic Organic Pollutants Impact
- Environmental Toxicology and Ecotoxicology
- Nanoparticles: synthesis and applications
- Effects and risks of endocrine disrupting chemicals
- Animal testing and alternatives
- Fish Ecology and Management Studies
- Adipose Tissue and Metabolism
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Per- and polyfluoroalkyl substances research
- Atmospheric chemistry and aerosols
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Computational Drug Discovery Methods
- Microplastics and Plastic Pollution
- Genetics, Aging, and Longevity in Model Organisms
- Carcinogens and Genotoxicity Assessment
- Single-cell and spatial transcriptomics
- CAR-T cell therapy research
- Physiological and biochemical adaptations
- Neuroinflammation and Neurodegeneration Mechanisms
- Heavy Metal Exposure and Toxicity
- Nitric Oxide and Endothelin Effects
- Environmental Chemistry and Analysis
- Anesthesia and Neurotoxicity Research
Incyte (United States)
2020-2025
Nuffield Orthopaedic Centre
2025
University of Oxford
2025
Oregon State University
2015-2024
University of California, Los Angeles
2018-2023
La Trobe University
2015-2022
Cook Children's Medical Center
2015-2020
Research Triangle Park Foundation
2017-2019
Society of Environmental Toxicology and Chemistry
2017-2019
Environmental Protection Agency
2017-2019
Nanomaterials are highly dynamic in biological and environmental media. A critical need for advancing health safety research nanomaterials is to identify physical chemical transformations that affect the nanomaterial properties their toxicity. Silver nanoparticles, one of most toxic well-studied nanomaterials, readily react with sulfide form Ag(0)/Ag2S core-shell particles. Here, we show sulfidation decreased silver nanoparticle toxicity four diverse types aquatic terrestrial eukaryotic...
There are tens of thousands man-made chemicals in the environment; inherent safety most these is not known. Relevant biological platforms and new computational tools needed to prioritize testing with limited human health hazard information. We describe an experimental design for high-throughput characterization multidimensional vivo effects power evaluate trends relating commonly cited chemical predictors. evaluated all 1060 unique U.S. EPA ToxCast phase 1 2 compounds using embryonic...
<h2>Abstract</h2> The potential of the developing zebrafish model for toxicology and drug discovery is limited by inefficient approaches to manipulating chemically exposing embryos—namely, manual placement embryos into 96- or 384-well plates exposure while still in chorion, a barrier poorly characterized permeability enclosing embryo. We report automated dechorionation 1600 at once 4 h postfertilization (hpf) dechorionated 96-well 6 hpf. process removed ≥95% from their chorions with 2%...
New strategies are needed to address the data gap between bioactivity of chemicals in environment versus existing hazard information. We whether a high-throughput screening (HTS) system using vertebrate organism (embryonic zebrafish) can characterize chemical-elicited behavioral responses at an early, 24 hours post-fertilization (hpf) stage that predict teratogenic consequences later developmental stage. The was used generate full concentration-response profiles hpf across 1060 ToxCast™...
Benzo[a]pyrene (B[a]P) is a well-known genotoxic polycylic aromatic compound whose toxicity dependent on signaling via the aryl hydrocarbon receptor (AHR). It unclear to what extent detrimental effects of B[a]P exposures might impact future generations and whether transgenerational be AHR-dependent. This study examined developmental exposure 3 zebrafish. Zebrafish embryos were exposed from 6 120 h post fertilization (hpf) 5 10 μM raised in chemical-free water until adulthood (F0). Two F0...
Per- and polyfluoroalkyl substances (PFAS) are a class of widely used chemicals with limited human health effects data relative to the diversity structures manufactured. To help fill this gap, an extensive in vivo developmental toxicity screen was performed on 139 PFAS provided by US EPA. Dechorionated embryonic zebrafish were exposed 10 nominal water concentrations (0.015-100 µM) from 6 120 h post-fertilization (hpf). The embryos assayed for photomotor response (EPR), larval (LPR), 13...
The fish early-life stage (FELS) test (Organisation for Economic Co-operation and Development [OECD] guideline 210) is the primary used internationally to estimate chronic toxicity in support of ecological risk assessments chemical management programs. As part an ongoing effort develop efficient cost-effective alternatives FELS test, there a need identify describe potential adverse outcome pathways (AOPs) relevant toxicity. To this endeavor, authors outline illustrate overall strategy...
The mechanism of action silver nanoparticles (AgNPs) is unclear due to the particles' strong tendency agglomerate.Preventing agglomeration could offer precise control physicochemical properties that drive biological response AgNPs.In an attempt agglomeration, we exposed zebrafish embryos AgNPs 20 or 110 nm core size, and polypyrrolidone (PVP) citrate surface coatings in media varying ionic strength.AgNPs remained unagglomerated 62.5 μM CaCl 2 (CaCl ) ultrapure water (UP), but not standard...
Nanoparticle safety is usually determined using solutions of individual particles that are free additives. However, the size-dependent properties nanoparticles can be readily altered through interactions with other components in a mixture. In applications, commonly combined surfactants or additives to increase dispersion enhance product performance. Surfactants might also influence biological activity nanoparticles; however, little known about such effects. We investigated on nanoparticle...
Abstract Purpose: T cells engineered to express a chimeric antigen receptor (CAR) are promising cancer immunotherapy. Such targeted therapies have shown long-term relapse-free survival in patients with B-cell leukemia and lymphoma. However, cytokine release syndrome (CRS) represents serious, potentially life-threatening side effect often associated CAR T-cell therapy. CRS manifests as rapid (hyper)immune reaction driven by excessive inflammatory release, including IFNγ IL6. Experimental...
Pavement sealcoat products contain high concentrations of unsubstituted polycyclic aromatic hydrocarbons (PAHs), but the assessment potential toxicological impact is limited without inclusion PAH derivatives. This study determined 23 PAHs, 11 molecular weight PAHs (MW302-PAHs), and 56 derivatives, including 10 methyl-PAHs (MPAHs), heterocyclic (Hetero-PAHs), 26 nitrated (NPAHs), oxygenated (OPAHs) in coal tar- asphalt-based time point scrapes. Inclusion MW302-PAHs resulted an increase...
How artificial environmental cues are biologically integrated and transgenerationally inherited is still poorly understood. Here, we investigate the mechanisms of inheritance reproductive outcomes elicited by model chemical Bisphenol A in C. elegans. We show that (BPA) exposure causes derepression an epigenomically silenced transgene germline for 5 generations, regardless ancestral response. Chromatin immunoprecipitation sequencing (ChIP-seq), histone modification quantitation,...
Per- and polyfluoroalkyl substances (PFAS) are ubiquitously detected in the environment, some pose significant human environmental health concerns globally. While PFAS induce adverse effects, relatively few toxicological studies adequately address broad structural diversity of this chemical class. In current study, we evaluated 58 individual spanning 14 subclasses 2 mixtures at single concentrations for developmental toxicity zebrafish using highly sensitive behavior endpoints. Following...