A5018947409- Advanced MRI Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- MRI in cancer diagnosis
- Cancer, Hypoxia, and Metabolism
- Medical Imaging Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Gene expression and cancer classification
- NMR spectroscopy and applications
- Breast Cancer Treatment Studies
- Spondyloarthritis Studies and Treatments
- Advanced NMR Techniques and Applications
- Bone and Joint Diseases
- Cancer, Lipids, and Metabolism
- Advanced Neuroimaging Techniques and Applications
- Electron Spin Resonance Studies
- Radiology practices and education
- Dermatology and Skin Diseases
- Hematological disorders and diagnostics
- Salivary Gland Disorders and Functions
- Lysosomal Storage Disorders Research
- Meat and Animal Product Quality
- Atomic and Subatomic Physics Research
- Interdisciplinary Research and Collaboration
- Psoriasis: Treatment and Pathogenesis
- Acne and Rosacea Treatments and Effects
Norwegian University of Science and Technology
2009-2023
Trondheim Kommune
2023
TrønderEnergi (Norway)
2013
St Olav's University Hospital
2009
Molde Hospital
2009
SINTEF
1998-2004
Abstract High‐resolution magic angle spinning (HR MAS) may develop into a new diagnostic tool for studying intact tissue samples, and several types of cancer have been investigated with promising results. In this study HR MAS spectra breast from 10 patients compared to conventional high‐resolution perchloric acid extracts the same type. The show resolution comparable extracts, two‐dimensional techniques lead identification majority constituents. More than 30 different metabolites detected...
Breast cancer is the most frequent form of in women and improved diagnostic methods are desirable. Malignant cells have altered metabolism metabolic mapping might become a tool diagnostics. High-resolution magic angle spinning (HR MAS) MR spectroscopy tissue biopsies provides detailed information on composition. The 600 MHz 1H HR MAS spectra were acquired breast from 85 patients adjacent non-involved 18 these patients. Tissue specimens investigated by microscopy after analysis. resulting...
Absolute quantitative measures of breast cancer tissue metabolites can increase our understanding biological processes. Electronic REference To access In vivo Concentrations (ERETIC) was applied to high resolution magic angle spinning MR spectroscopy (HR MAS MRS) quantify in intact samples. The ERETIC signal calibrated using solutions creatine and TSP. largest relative errors the method were 8.4%, compared 4.4% for HR MRS TSP as a standard. same experimental procedure samples from patients...
Axillary lymph node status together with estrogen and progesterone receptor are important prognostic factors in breast cancer. In this study, the potential of using MR metabolomics for prediction these was evaluated. Biopsies from cancer patients (n = 160) were excised during surgery analyzed by high resolution magic angle spinning spectroscopy (HR MAS MRS). The spectral data preprocessed variable stability (VAST) scaled, training test sets generated Kennard-Stone SPXY sample selection...
Abstract Background Combining gene expression microarrays and high resolution magic angle spinning magnetic resonance spectroscopy (HR MAS MRS) of the same tissue samples enables comparison transcriptional metabolic profiles breast cancer. The aim this study was to explore potential combining these two different types information. Methods Breast cancer from 46 patients analyzed by HR MRS followed microarrays. Two strategies were used combine data; first using multivariate analyses identify...
Increased concentrations of choline-containing compounds are frequently observed in breast carcinomas, and may serve as biomarkers for both diagnostic treatment monitoring purposes. However, underlying mechanisms the abnormal choline metabolism poorly understood. The choline-derived metabolites were determined xenografted primary human representing basal-like luminal-like subtypes. Quantification fresh frozen tissue was performed using high-resolution magic angle spinning magnetic resonance...
Purpose This study aimed to evaluate whether MR metabolic profiling can be used for prediction of long‐term survival and monitoring treatment response in locally advanced breast cancer patients during neoadjuvant chemotherapy (NAC). Methods: High resolution magic angle spinning (HR MAS) spectra pre‐ post‐treatment biopsies from 33 were acquired. Tissue concentrations choline‐containing metabolites (tCho), glycine taurine assessed using electronic reference access vivo concentration (ERETIC)...
Today's clinical diagnostic tools are insufficient for giving accurate prognosis to breast cancer patients. The aim of our study was examine the tumor metabolic changes in patients with locally advanced caused by neoadjuvant chemotherapy (NAC), relating these treatment response and long-term survival. Patients (n = 89) participating a randomized open-label multicenter were allocated receive either NAC as epirubicin or paclitaxel monotherapy. Biopsies excised pre- post-treatment, analyzed...
In this study, the feasibility of high resolution magic angle spinning (HR MAS) magnetic resonance spectroscopy (MRS) small tissue biopsies to distinguish between tumor and non-involved adjacent was investigated. With current methods, delineation borders during breast cancer surgery is a challenging task for surgeon, significant number re-surgeries occur. We analyzed 328 samples from 228 patients using HR MAS MRS. Partial least squares discriminant analysis (PLS-DA) applied discriminate...
Breast cancer is a heterogeneous disease with variable prognosis. Clinical factors provide some information about the prognosis of patients breast cancer; however, there need for additional to stratify improved and more individualized treatment. The aim this study was examine relationship between metabolite profiles tissue 5‐year survival. Biopsies from ( n = 98) were excised during surgery analyzed by high‐resolution magic angle spinning MRS. data multivariate principal component analysis...
Dysregulated choline metabolism is a well-known feature of breast cancer, but the underlying mechanisms are not fully understood. In this study, metabolomic and transcriptomic characteristics large panel human cancer xenograft models were mapped, with focus on metabolism. Tumor specimens from 34 patient-derived collected divided in two. One part was examined using high-resolution magic angle spinning (HR-MAS) MR spectroscopy while another analyzed gene expression microarrays. Expression data...
Metabolic profiling of intact tumor tissue by high-resolution magic angle spinning (HR MAS) MR spectroscopy (MRS) provides important biological information possibly useful for clinical diagnosis and development novel treatment strategies. However, generation high-quality data requires that sample handling from surgical resection until analysis is performed using systematically validated procedures. In this study, we investigated the effect postsurgical freezing delay time on global metabolic...
High-resolution magic angle proton magnetic resonance spectroscopy (HR 1H MAS MRS) provides a broad metabolic mapping of intact tumor samples and allows for microscopy investigations the after spectra acquisition. Experimental studies have suggested that method can be used detection apoptosis, but this has not been investigated in clinical setting so far. We explored hypothesis cervical cancers by searching metabolites associated with apoptosis were influenced other histopathological...
MR spectroscopy of intact biopsies can provide a metabolic snapshot the investigated tissue. The aim present study was to explore pattern uninvolved skin, psoriatic skin and corticosteroid treated skin.The three types biopsy samples were excised from patients with psoriasis (N = 10). Lesions evaluated clinically, tissue analyzed by one-dimensional 1H spectroscopy. Relative levels calculated for nine metabolites. Subsequently, relative amounts epidermis, dermis subcutaneous scored...
This study was conducted in order to elucidate metabolic differences between human rectal cancer biopsies and colorectal HT29, HCT116 SW620 xenografts by using high-resolution magnetic angle spinning (MAS) resonance spectroscopy (MRS) for determination of the most appropriate xenograft model preclinical MR studies. A further aim investigate changes following irradiation HT29 xenografts. HR MAS MRS tissue samples from were obtained with a Bruker Avance DRX600 spectrometer analyzed principal...
Recognizing the link between altered tumor metabolism and disease aggressiveness, this study aimed to identify associations metabolic profiles therapeutic outcome in locally advanced rectal cancer (LARC).Pretreatment from 54 LARC patients receiving combined-modality neoadjuvant treatment surgery were acquired by high-resolution magic angle spinning magnetic resonance spectroscopy (HR MAS MRS). Metabolite concentrations correlated TNM presence of disseminated cells (DTC) at diagnosis, ypTN...
Tumor hypoxia contributes to therapy resistance and metastatic progression of locally advanced rectal cancer (LARC). We postulated that the tumor mitochondrial metabolism, manifested by reactive oxygen species (ROS) DNA (mtDNA) damage, reflects how hypoxic conditions connect cancer-induced systemic inflammation poor outcome. Levels ROS mtDNA damage were analyzed in three colorectal (CRC) cell lines cultured for 24 hours under normoxia (21% O2) or (0.2% serum sampled at time diagnosis from 35...