A5011308113- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Advanced Proteomics Techniques and Applications
- Advanced Steganography and Watermarking Techniques
- Traditional Chinese Medicine Analysis
- Glycosylation and Glycoproteins Research
- Gut microbiota and health
- Peptidase Inhibition and Analysis
- Metabolomics and Mass Spectrometry Studies
- Advanced Chemical Sensor Technologies
- Analytical chemistry methods development
- Chronic Kidney Disease and Diabetes
- Cyclopropane Reaction Mechanisms
- Per- and polyfluoroalkyl substances research
- Sulfur-Based Synthesis Techniques
- Industrial Vision Systems and Defect Detection
- Advanced Data Compression Techniques
- Amino Acid Enzymes and Metabolism
- Ion-surface interactions and analysis
- Digital Media Forensic Detection
- Advanced X-ray and CT Imaging
- Pluripotent Stem Cells Research
- Neurological Disease Mechanisms and Treatments
- Ubiquitin and proteasome pathways
- Probiotics and Fermented Foods
Chongqing Medical University
2015-2024
Zhejiang University
2007-2024
The Affiliated Yongchuan Hospital of Chongqing Medical University
2015-2024
First Affiliated Hospital of Xi'an Jiaotong University
2015-2024
Macau University of Science and Technology
2024
Molina Center for Energy and the Environment
2022
Zhejiang Sci-Tech University
2016-2018
China XD Group (China)
2018
South China Agricultural University
2015-2017
Beijing Aerospace Flight Control Center
2011
In contrast to the detailed molecular knowledge available on anthocyanin synthesis, little is known about its catabolism in plants. Litchi (Litchi chinensis) fruit lose their attractive red color soon after harvest. The mechanism leading quick degradation of anthocyanins pericarp not well understood. An enzyme (ADE) was purified homogeneity by sequential column chromatography, using partially from litchi as a substrate. ADE, 116 kD urea SDS-PAGE, identified laccase (ADE/LAC). full-length...
Summary The tafazzin ( TAZ ) gene is highly conserved from yeast to humans, and the taz1 null mutant shows alterations in cardiolipin (CL) metabolism, mitochondrial dysfunction stabilization of supercomplexes similar those found Barth syndrome, a human disorder resulting loss tafazzin. We have previously shown that Δ, which cannot remodel CL, ethanol‐sensitive at elevated temperature. In current report, we show response ethanol, CL mutants Δ as well crd1 synthesize exhibited increased...
A novel bromine-isotope probe named D-BPBr with stereodynamic chiral recognition characteristics was developed for the labeling, separation, and detection of trace amino acids amino-containing metabolites. Fourteen enantiomeric pairs could be successfully separated quantified on a reverse-phase C18 column an HPLC-MS/MS system after labeling. The chromatographic resolution d,l-amino acid enantiomers ranged from 1.14 to 8.83 l-amino derivative always eluting prior corresponding d-enantiomer....
myo-Inositol-3-phosphate synthase (MIPS) plays a crucial role in inositol homeostasis. Transcription of the coding gene INO1 is highly regulated. However, regulation enzyme not well defined. We previously showed that MIPS indirectly inhibited by valproate, suggesting post-translationally Using (32)Pi labeling and phosphoamino acid analysis, we show yeast phosphoprotein. Mass spectrometry analysis identified five phosphosites, three which are conserved human MIPS. Analysis...
Objective: Pirarubicin (THP), one of the anthracycline anticancer drugs, is widely used in treatment various cancers, but its cardiotoxicity cannot be ignored. Schisandrin B (SchB) has ability to upregulate cellular antioxidant defense mechanism and promote mitochondrial function status. However, it not been reported whether can resist THP-induced cardiotoxicity. The aim this study was investigate effect SchB on THP mechanism. Methods: rat model induced by established, performed at same...
Pirarubicin (THP) is one of the most commonly used antineoplastic drugs in clinical practice. However, its application limited due to toxic and heart‑related side effects. It has been reported that oxidative stress, inflammation apoptosis are closely associated with cardiotoxicity caused by pirarubicin (CTP). Additionally, it also scutellarein (Sc) exerts anti‑inflammatory, antioxidant, cardio‑cerebral vascular protective anti‑apoptotic properties. Therefore, present study aimed investigate...
Screening of characteristic biomarkers from chiral amino-containing metabolites in biological samples is difficult and important for the noninvasive diagnosis gastric cancer (GC). Here, an enantiomeric pair chlorine-labeled probes d-BPCl l-BPCl was synthesized to selectively label d- l-amino-containing samples, respectively. Incorrect structural annotations were excluded according 3:1 abundance ratio natural chlorine isotopes (35Cl 37Cl) derived probes. A sensitive C18 HPLC-QQQ-MS/MS method...
Oncogenic KIT or PDGFRA receptor tyrosine kinase (RTK) mutations are compelling therapeutic targets in gastrointestinal stromal tumors (GIST), and treatment with the KIT/PDGFRA inhibitor imatinib is standard of care for patients metastatic GIST. Most GISTs eventually acquire resistance due to secondary domain, but it unclear whether these genomic mechanisms require other cellular adaptations create a clinically meaningful imatinib-resistant state. Using phospho-RTK immunoblot assays, we...
Improved mesothelioma patient survival will require development of novel and more effective pharmacological interventions. TP53 genomic mutations are uncommon in mesothelioma, recent data indicate that p53 remains functional, therefore is a potential therapeutic target these cancers. In addition, the tumour suppressor NF2 inactivated by mechanisms than 80% causing upregulation FAK activity. Because negative regulator p53, regulation FAK–p53–MDM2 signalling loops were evaluated. Interactions...
Qualitative and quantitative analysis of N-glycans by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is hampered mainly the low ionization efficiency analytes their poor cocrystallization with traditional organic acid matrices. In present study, a combination strategy reactive catalytic matrices (CSRCM) was proposed for on-target derivatization detection reducing N-glycans: novel matrix, i.e., 2,5-dihydroxybenzohydrazide (DHBH), having skeleton structure similar to...
Human tissue plasminogen activator (tPA) belongs to the serine protease family. It converts into plasmin and is used clinically treat thrombosis. tPA composed of 527 amino acids residues contains 17 disulfide bonds. Escherichia coli has been only rarely for efficient production recombinant tPA. However, functional expression full-length that multiple bonds on an industrial scale remains challenging. Here, we describe soluble characterization by auto-induction in E. coli. We achieved optimal...
Induced pluripotent stem cells (iPS) represent an innovative source for the standardized in vitro generation of macrophages (Mφ). Mφ show great promise disease pathogenesis, particularly tuberculosis. However, there is no information about human iPS-derived (hiPS) (hiPS-Mφ) response to tuberculosis infection. In present study, derived from hiPS were established via embryoid body (EB) formation by using feeder-free culture conditions, and monocyte cell line THP-1 (THP-1-Mφ) was used as...
An increasing number of investigations have revealed that podocytes play a crucial role in the development and progression diabetic nephropathy (DN). Quercetin (2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one) is most common flavonol diet one prominent dietary antioxidants, which might protective effect on DN. The present study was designed to investigate quercetin podocyte impairment rat model DN, as well underlying molecular mechanisms. All rats were induced by single...
Studies have shown that the inositol biosynthetic pathway and enzyme glycogen synthase kinase-3 (GSK-3) are targets of mood-stabilizing drugs lithium valproate. However, a relationship between these has not been previously described. We hypothesized GSK-3 may play role in synthesis, loss lead to depletion, thus providing mechanistic link two drug targets. Utilizing yeast Saccharomyces cerevisiae gsk-3Delta quadruple-null mutant, which all four genes encoding homologues mammalian disrupted,...
// Peipei Yang 1, * , Weicai Chen Xuhui Li 2 Grant Eilers 3 Quan He 1 Lili Liu Yeqing Wu Yuehong Wei Yu Jonathan A. Fletcher Wen-Bin Ou 2, Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, College Life Sciences, Sci-Tech University, Hangzhou, China Applied Enzymology, Yangtze Delta Region Institute Tsinghua Jiaxing, Zhejiang, Department Pathology, Brigham Women's Hospital Harvard Medical School, Boston, MA, USA These authors contributed equally to this work...
Glycosylation is an important post-translational modification of proteins, and abnormal glycosylation involved in a variety diseases. Accurate rapid profiling N-glycans by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) still technically challenging hampered mainly drift instrument, manual identification spectrum peaks, poor cocrystallization with traditional matrices besides low ionization efficiency analytes. In the present study, parallel...
Ambient electric arc ionization mass spectrometry (AEAI-MS) was developed for the analysis of various organic compounds having a wide range polarities, ranging from non-polar to highly polar species.
Glycans recently attracted considerable attention as the proposal of cross-reactive carbohydrate determinants for food allergy. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) is powerful in analyzing biomolecules, while its applications glycans are still challenging. Herein, a novel reactive matrix-assisted desorption/ionization (MALDI) matrix, 2-hydrazinoterephthalic acid, was rationally designed and synthesized. It provides uniform co-crystallization with only...