A5012271205- Cardiac Valve Diseases and Treatments
- Cardiac Structural Anomalies and Repair
- Aortic Disease and Treatment Approaches
- Transplantation: Methods and Outcomes
- Neonatal Respiratory Health Research
- Infective Endocarditis Diagnosis and Management
- Cardiovascular Function and Risk Factors
- Xenotransplantation and immune response
- Cell Adhesion Molecules Research
- Cardiac and Coronary Surgery Techniques
- Atrial Fibrillation Management and Outcomes
- Surgical Sutures and Adhesives
- Organ Transplantation Techniques and Outcomes
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Mechanical Circulatory Support Devices
- Cardiac Imaging and Diagnostics
- Hemostasis and retained surgical items
- Cardiac Arrhythmias and Treatments
- Vascular Procedures and Complications
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Polymer Surface Interaction Studies
- TGF-β signaling in diseases
- Infectious Aortic and Vascular Conditions
- NF-κB Signaling Pathways
San Francisco General Hospital
2023
University of California, San Francisco
2010-2022
Fistula Foundation
2020
University of Nebraska Medical Center
2000-2003
University of Nebraska at Omaha
2003
Loma Linda University
1994-1997
Loma Linda University Medical Center
1994-1997
Pitié-Salpêtrière Hospital
1997
Sorbonne Université
1997
University of Pittsburgh Medical Center
1994
Squamous metaplasia (SM) is common in smokers and associated with airway obstruction chronic obstructive pulmonary disease (COPD). A major mechanism of COPD thickening the small walls. We asked whether SM actively contributes to wall through alteration epithelial-mesenchymal interactions COPD. Using immunohistochemical staining, morphometry, fibroblast culture lung samples from patients; genome-wide analysis an vitro model SM; modeling human interactions, we provide evidence that SM,...
The airway is a primary portal of entry for noxious environmental stimuli that can trigger remodeling, which contributes significantly to obstruction in chronic obstructive pulmonary disease (COPD) and asthma. Important pathologic components remodeling include fibrosis abnormal innate adaptive immune responses. positioning fibroblasts interstitial spaces suggests they could participate both chemokine regulation the trafficking cells such as dendritic cells, are crucial antigen-presenting...
Therapeutic targeting of an extended-closed conformation the integrin αvβ8 inhibits TGF-β activation and ameliorates symptoms experimental airway disease in mice.
The integrin αvβ8 is a cell surface receptor for the latent domain (LAP) of multifunctional cytokine TGF-β. Through its association with LAP, TGF-β maintained in form that must be activated to function. Binding subsequent metalloproteolytic cleavage LAP represents major mechanism activation vivo. Altered expression β8 subunit (ITGB8) found human chronic obstructive pulmonary disease, cancers, and brain vascular malformations. We have previously shown proinflammatory interleukin-1β (IL-1β)...
CCL20 is the only chemokine ligand for receptor CCR6, which expressed by critical antigen presenting cells, dendritic cells. Increased expression of likely involved in increased recruitment cells observed fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). proinflammatory cytokine IL-1β. We have determined that IL-1β-dependent also dependent on multifunctional TGF-β. TGF-β a latent form must be activated to function, and activation achieved through binding...
Abstract The purpose of this study is to evaluate the effectiveness P15 cell-binding peptide treated ePTFE vascular grafts in vitro and vivo. was covalently immobilized onto by an atmospheric plasma coating method. In cell growth properties were studied using primary human umbilical vein endothelial cells (HUVECs) artery smooth muscle (HUASMCs). X-ray photoelectron spectroscopy aminoacid analysis used analyze surface characteristics untreated grafts. showed that effectively promoted adhesion...
CoSeal mark surgical sealant (CoSeal) was evaluated for inhibiting suture line bleeding using a canine iliac PTFE graft model. Both arteries of 12 heparinized canines were grafted with PTFE. applied to the lines one in each animal. The contra-lateral served as control and controlled gauze pressure (tamponade). cross-clamps removed 30 s following application CoSeal. Times hemostasis volume blood loss at site determined. Compared tamponade control, significantly reduced time (average 5 min vs....
Integrin αvβ8 is a critical regulator of transforming growth factor β activation in vasculogenesis during development, immune regulation, and endothelial/epithelial-mesenchymal homeostasis. Recent studies have suggested roles for integrin β8 the pathogenesis chronic obstructive pulmonary disease, brain arteriovenous malformations, select cancers (Araya, J., Cambier, S., Markovics, J. A., Wolters, P., Jablons, D., Hill, Finkbeiner, W., Jones, K., Broaddus, V. C., Sheppard, Barzcak, Xiao, Y.,...
The severity of aortic stenosis (AS) has traditionally been graded by measuring hemodynamic parameters transvalvular pressure gradient, ejection jet velocity, or estimating valve orifice area. Recent research highlighted limitations these criteria at effectively grading AS in presence left ventricle (LV) dysfunction. We hypothesized that simulations coupling the aorta and LV could provide meaningful insight into myocardial biomechanical derangements accompany AS. A realistic finite element...
Objective To assess the use of donor pigs with cellular chimerism for prevention acute rejection modest immune suppression. The clinical pig organ xenografts is currently precluded by severe rejection, which resists standard Summary Background Data For long-term survival xenografts, suppression significantly greater than used allografts would be necessary, leaving recipient deficient and at increased risk infections. Induction tolerance tissue accommodation could enhance xenograft but lead...
We evaluated the completeness and extent of regional sympathetic denervation left ventricle after epicardial painting with phenol in anesthetized dogs. In a region encircled by phenol, effect electrical stimulation efferent fibers on myocardial contractility coronary vascular resistance was completely abolished within 30 min. Denervation extended to untreated regions innervated crossing line. For at least 4 h application, intravenous infusion isoproterenol or arterial tyramine increased...
Abstract The data on function and patency of prosthetic vascular grafts in various clinical settings are limited. purpose this vivo study was to compare the P15‐coated expanded polytetrafluoroethylene (ePTFE) uncoated ePTFE sheep. P15 cell‐binding peptide covalently immobilized onto surface by a novel atmospheric plasma coating method. We evaluated amount neointimal tissue ingrowth present at arterial venous sides anastomoses degree endothelial cell resurfacing luminal graft. Four two...