A5018839044- Dental materials and restorations
- Dental Implant Techniques and Outcomes
- Orthodontics and Dentofacial Orthopedics
- Neuroinflammation and Neurodegeneration Mechanisms
- Inflammasome and immune disorders
- Temporomandibular Joint Disorders
- Intensive Care Unit Cognitive Disorders
- Sepsis Diagnosis and Treatment
- S100 Proteins and Annexins
- Dental Erosion and Treatment
- Bone and Dental Protein Studies
- Barrier Structure and Function Studies
- Traumatic Brain Injury and Neurovascular Disturbances
- Respiratory Support and Mechanisms
- Immune Response and Inflammation
- Bacteriophages and microbial interactions
- Neonatal and fetal brain pathology
- Enzyme Production and Characterization
- Fungal and yeast genetics research
- Bone Tissue Engineering Materials
- Bacterial Genetics and Biotechnology
- Plant Virus Research Studies
- Oral microbiology and periodontitis research
- Microbial Metabolites in Food Biotechnology
- Plant Disease Resistance and Genetics
Binzhou People's Hospital
2020-2025
Guangdong Academy of Medical Sciences
2019-2024
Southern Medical University
2019-2024
Guangdong Provincial People's Hospital
2019-2024
Chinese Academy of Sciences
2017-2024
Center for Excellence in Molecular Plant Sciences
2017-2024
University of Chinese Academy of Sciences
2017-2024
Qingdao University
2004-2023
State Key Laboratory of Medicinal Chemical Biology
2023
Affiliated Hospital of Qingdao University
2019-2023
Mycoviruses are viruses that infect fungi, and hypovirulence-associated mycoviruses have the potential to control fungal diseases. However, it is unclear how mycovirus-mediated hypovirulent strains live survive in field, no mycovirus has been applied for field crop protection. In this study, we found a previously identified small DNA (SsHADV-1) can convert its host, Sclerotinia sclerotiorum, from typical necrotrophic pathogen beneficial endophytic fungus. SsHADV-1 downregulates expression of...
To explore the involvement of NOD-like receptor protein 3 (NLRP3) inflammasome and M1 macrophage in root resorption (RR).A rat RR model was established by excessive orthodontic force. After different force-loading time, expression levels NLRP3, caspase-1, interleukin-1β (IL-1β) distribution macrophages were analysed immunohistochemistry immunofluorescence staining vivo. Then, mechanism NLRP3 activation further verified human periodontal ligament cell (hPDLC) co-culture system vitro. The...
This article aims to investigate the role of Helicobacter Pylori (HP) CagA+ strains affected colorectal lesion via gut microbiota. 6-week C57BL/6J mice were divided into: (a) HP group undergoing administration by gavage at 0.2 mL for 10 days; (b) CagA- (c) control intragastrically given brian heart infusion (BHI) medium days. Gastric mucosa was collected Giemsa staining, and hematoxylin eosin (H&E) 16 S ribosomal RNA (rRNA) sequencing immunohistochemistry Major Histocompatibility Complex...
Background The NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome has been identified as an important mediator of blood–brain-barrier disruption in sepsis-associated encephalopathy (SAE). However, no information is available concerning the critical upstream regulators SAE. Methods Lipopolysaccharide (LPS) was used to establish vitro model blood–brain barrier (BBB) vivo Disruption BBB integrity assessed by measuring expression levels tight-junction proteins. NLRP3...
Botrytis cinerea, a ubiquitous necrotrophic plant-pathogenic fungus, is responsible for grey mold and rot disease in very wide range of plant species. Subtilisin-like proteases (or subtilases) are diverse family serine present many organisms reported to have broad spectrum biological functions. Here, we identified two genes encoding subtilisin-like (Bcser1 Bcser2) the genome B. both which contain an inhibitor I9 domain peptidase S8 domain. The expression levels Bcser1 Bcser2 increased during...
To evaluate the effect of casein phosphopeptide-amorphous calcium phosphate tooth mousse on remineralization bovine incisor by circularly polarized images.Eighty incisors, each with a 4 x mm artificially demineralized area, were used. The samples divided into four groups: Group A, mousse; B, fluoride toothpaste; C, and D, no treatment. Circularly images taken after specimens treated for 3, 6, 9, or 12 weeks, size area mean grey level measured. Data analysis was done using repeated measures...
Blood-brain barrier (BBB) impairment plays a significant role in the pathogenesis of sepsis-associated encephalopathy (SAE). However, molecular mechanisms are poorly understood. In present study, we aimed to investigate regulatory relationship between Janus kinase/signal transducers and activators transcription (JAK/STAT) signaling pathway, microRNA (miR)-181b its target genes sepsis vivo vitro.Four rat models (sham, sepsis, plus STAT3 inhibitor (Stattic), miR-181b [sepsis + anta-miR-181b])...
Abstract Background Bioethanol production from various starchy materials has received much attention in recent years. α-Amylases are key enzymes the bioconversion process of biomass to biofuels, food or other products. The properties thermostability, pH stability, and Ca-independency important development such fermentation process. Results A novel Flavobacteriaceae Sinomicrobium α-amylase (FSA) was identified characterized genomic analysis a species. It is closely related with archaeal...
Histone methylation is widely present in animals, plants and fungi, the modification of histone H3 has important biological functions. Methylation Lys9 (H3K9) been proven to regulate chromatin structure, gene silencing, transcriptional activation, plant metabolism other processes. In this work, we investigated functions a H3K9 methyltransferase BcDIM5 Botrytis cinerea, which contains PreSET domain, SET domain PostSET domain. Characterization knockout transformants showed that hyphal growth...
Sepsis is a serious systemic inflammatory response syndrome caused by infection, with an extremely high mortality rate. Peripheral blood mononuclear cells (PBMCs) played key role in the immune against whose components and functions were altered radically Sepsis. Here, we wondered to characterize alteration of PBMCs sepsis at single-cell transcriptional level.We isolated from seven septic patients four donors. Based on BD Rhapsody, generated RNA sequencing, cell types clustered named...
Sepsis-induced myocardial dysfunction (SMD) is the major cause of death in sepsis. Nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3)-mediated pyroptosis contributes to occurrence and development SMD. Although Apelin confers direct protection against SMD, potential mechanisms remain unclear. This study aimed determine whether protects SMD via regulation NLRP3-mediated cardiomyocytes. Experimental was induced wild-type (WT) control mice knockout (Apelin
Due to the important role of rifamycin in curing tuberculosis infection, study on has never been stopped. Although RifZ, which locates within biosynthetic cluster, recently characterized as a pathway-specific regulator for biosynthesis, little is known about regulation export.In this work, we proved that expression efflux pump (RifP) was regulated by RifQ, TetR-family transcriptional regulator. Deletion rifQ had impact bacterial growth, but resulted improved production, consistent with...
Acute type A aortic dissection (TAAD) is cardiovascular emergency and requires surgical interventions. In-hospital mortality rate of surgical-treated TAAD patients remains high. We aim to examine the prognostic implications peri-operative parameters identify high-risk patient for in-hospital mortality.A total 264 surgically treated were included in this study. The association between examined.Thirty (11.36%) died during hospitalization. Patients with higher Apache II score had a...
Refractory hypoxemia is the main symptom of acute respiratory distress syndrome (ARDS). Low tidal volume ventilation routinely applied in clinical practice to correct hypoxemia, which aims prevent ventilator‑induced lung injury. However, this strategy inevitably leads hypercapnia. Our previous study demonstrated that hypercapnia aggravated cognitive impairment hypoxemic rats; however, underlying mechanism remains unclear. The aim present was investigate whether exacerbates blood‑brain...
Rifamycin and its derivatives are particularly effective against the pathogenic mycobacteria Mycobacterium tuberculosis leprae Although biosynthetic pathway of rifamycin has been extensively studied in Amycolatopsis mediterranei, little is known about regulation biosynthesis. Here, an vivo transposon system was employed to identify genes involved production A. mediterranei U32. In total, nine rifamycin-deficient mutants were isolated, among which three had inserted AMED_0655 (rifZ, encoding...
Amycolatopsis mediterranei U32 is an industrial producer of rifamycin SV, whose derivatives have long been the first-line antimycobacterial drugs. In order to perform genetic modification in this important strain, a lot efforts made past decades and homologous recombination-based method was successfully developed our laboratory, which, however, requires employment antibiotic resistance gene for positive selection did not support convenient markerless deletion. Here study, clustered regularly...
The enzyme 6-phospho-β-glucosidase is an important member of the glycoside hydrolase family 1 (GH1). However, its catalytic mechanisms, especially key residues determining substrate specificity and affinity, are poorly understood. A metagenome-derived gene sequence, encoding a novel designated Pbgl25-217, was isolated characterized. optimal conditions for enzymatic activity were 37°C pH 7; Ca(2+), Mg(2+), Mn(2+) stabilized whereas Ni(2+), Fe(2+), Zn(2+), Cu(2+), Fe(3+) inhibited activity. Km...