Abstract Human mesenchymal stem cells (hMSCs) are considered a highly promising candidate cell type for cell‐based tissue engineering and regeneration because of their self‐renewal multi‐lineage differentiation characteristics. Increased levels reactive oxygen/nitrogen species (ROS/RNS) associated with injury inflammation, impact number cellular processes, including adhesion, migration, proliferation, have been linked to senescence in MSCs, potentially compromising activities. Naturally...

<italic>In situ</italic> N-doped mesoporous carbon nanofibers synthesized by depositing magnesium hydroxide (Mg(OH)₂) into polyacrylonitrile (PAN) and combining carbonization with etching process exhibit excellent supercapacitive performance.

Abstract Introduction There have been limited advances in the treatment of bone and joint infections, which currently involves a combination surgery antibiotic administration. is timely need orthopedics to develop more effective less invasive forms antimicrobial prophylaxis treatment. The antibacterial effect adult tissue-derived mesenchymal stem cells (MSCs) has recently investigated against Escherichia coli Staphylococcus aureus . main mechanism action postulated be via MSC production...

Abstract Background Prenatal inflammation exposure (PIE) can increase the disease susceptibility in offspring such as lung cancer. Our purpose was to investigate mechanisms of PIE on Methods BALB/c mice were exposed lipopolysaccharide (LPS), and then, their intraperitoneally instilled with urethane establish two-stage cancer carcinogenesis model. At 48 weeks age, killed tissues collected for HE, immunohistochemistry, immunofluorescence, Luminex MAGPIX®-based assays. CD11b + F4/80...

AimsThe relationship between biodiversity and ecological stability is a long-standing issue in ecology. Current diversity–stability studies, which have largely focused on species diversity, often report an increase the of aggregate community properties with increasing diversity. Few studies examined linkage phylogenetic another important dimension biodiversity, stability. By taking evolutionary history into account, diversity may better capture traits niches than relate to temporal In this...

Amelogenins are a group of extracellular enamel matrix proteins which believed to be involved in the regulation size and habits forming crystals. The aim this study was compare solubility properties several amelogenins at various pH (from 4.0 9.0) constant ionic strength (IS), examine influence buffer composition, IS, divalent metal ions (including Ca2+, Mg 2+ , Zn ) on amelogenin solubility. recombinant murine ("rM179") minimum near its isoelectric point increased rapidly below above,...

We have proposed that these nanosphere structures are functionally involved in the organization and control of initial enamel biomineralization at ultrastructural level. Based on observed hydrodynamic radii (18-20 nm diameter) computation suggests to be compounded some 100 amelogenin monomers, raising question as possible molecular mechanism for assembly such structures? recent dynamic light scattering experiments using recombinant murine M179, employing a newer size distribution algorithm...

Amelogenins are a group of extracellular enamel matrix proteins which believed to be involved in the regulation size and habit crystals. The aim this study was compare solubility properties several amelogenins various pH (4.0-9.0) solutions with an ionic strength (IS) 0.15 M using Micro BCA protein assay at 25 degrees C or 37 C. recombinant amelogenin rM179 lowest (0.7 mg/ml) close its isoelectric point it increased below above point. rM166 remained almost same (1-2 as rose from 6.0 9.0...

Abstract Myocardial ischaemia‐reperfusion (I/R) injury is a serious illness with high morbidity and mortality. Mounting evidence indicates the utility of sevoflurane (SEV) in treatment myocardial I/R injury. This study aimed to explore molecular mechanisms underlying protective action SEV against A rat model was established, rats were treated different concentrations SEV. MicroRNA‐203 (miR‐203) doublecortin (DCX) expression levels determined using reverse transcription‐quantitative...

Abstract Background Traumatic impacts to the articular joint surface are known lead cartilage degeneration, as in post-traumatic osteoarthritis (PTOA). Limited progress development of disease-modifying OA drugs (DMOADs) may be due insufficient mechanistic understanding human disease onset/progression and vitro models for therapeutic modeling. In this study, biomimetic hydrogels laden with adult mesenchymal stromal cells (MSC) used examine effects traumatic a model PTOA. We hypothesize that...

AbstractThe objective of the present study was to determine effect apatite crystals on activity amelogenin degrading enzymes in vitro. Current experimental data, together with previous reports support view that among different proteinases enamel extracellular matrix, serine proteinase(s) are responsible for massive degradation amelogenins during maturation stage. For our in-vitro experiments we used recombinant M179 as substrate and a “65%-satd. (NH4)2SO4” fraction proteins well chymotrypsin...

Drug resistance is a major impediment to the successful treatment of human cancers, including ovarian cancer. Vinblastine (VLB), an antimicrotubule agent, one chemotherapeutic drugs that exhibit in cancer patients. To determine protein factors are involved vinblastine cells, combination sample pre-fractionation and high-resolution 2-DE proteomic analysis was performed. Approximately 1200 proteins were detected quantitatively compared both nuclear/membrane cytosolic fractions. Sixty-nine from...

Objective. The study aimed to investigate the effect of anterior cervical surgery in treatment spinal cord injury without radiographic abnormalities (SCIWORAs) and analyze related factors affecting prognosis patients. Methods. A total 86 patients with SCIWORA who were admitted our hospital from June 2018 March 2021 selected as research subjects. According different methods by patients, they divided into control group (n = 38) observation 48). was treated conservative therapy, laminectomy...

Parkinson's disease (PD) is a well-known neurodegenerative disorder characterized by the degeneration of dopaminergic neurons, and oxidative stress neuroinflammation are also associated with pathogenesis PD. Mitochonic acid 5 (MA-5), an analogue indole-3-acetic acid, exerts key protective roles in inhibiting apoptosis, multiple diseases. However, whether MA-5 can be beneficial for PD remains unclear. Hence, aim this study was to investigate neuroprotective role In current study,...

Skeletal muscle stem cell (SMSC) transplantation has shown great therapeutical potential in repairing loss and dysfunction, but the acquisition is usually a traumatic procedure causing pain morbidity to donor. In this study, we investigated feasibility of isolating SMSCs from human orbicularis oculi (OOM), which routinely removed discarded during ophthalmic cosmetic surgeries. OOM fragments were harvested 18 female healthy donors undergoing upper eyelid plasties. Plastic-adherent cells...