Purpose: To characterize the morphology of neovascularization at disc (NVD) and elsewhere (NVE) in treatment-naïve or previously treated proliferative diabetic retinopathy (PDR) patients using optical coherence tomography (OCT) angiography. Methods: En face OCT angiograms NVD/NVE 40 eyes 33 with PDR were acquired RTVue XR Avanti OCT. The on was evaluated, activity determined by biomicroscopy fluorescein angiography (FA). In 12 that treatment-naïve, changes vessel area before after panretinal...

Purpose: We quantitatively analyzed the features of a radial peripapillary capillary (RPC) network visualized using wide-field montage optical coherence tomography (OCT) angiography in healthy human eyes. Methods: Twenty eyes 20 subjects were recruited. En face 3 × 3-mm OCT angiograms multiple locations posterior pole acquired RTVue XR Avanti, and images RPC created. To evaluate density, binarized skeletonized. The correlation between density retinal nerve fiber layer (RNFL) thickness...

To evaluate the differences in retinal circulation eyes of patients with type 2 diabetes no or early-stage diabetic retinopathy compared control eyes.Seventy-five nondiabetic and 194 mellitus were evaluated. The classified into two groups: 139 (139 patients) without (NDR) 55 (55 mild nonproliferative (NPDR). circulatory parameters measured laser Doppler velocimetry, factors that affect hemodynamics determined a cross-sectional population diabetes.The group-averaged blood velocity (V) flow...

The aim of this study was to determine whether NOX2, one the homologs NADPH oxidase, plays a role in neuronal cell death during retinal ischemia.Ischemia reperfusion (I/R) injury generated C57/BL6 and NOX2(-/-) mice by increasing intraocular pressure (IOP) 110 mm Hg for 40 minutes followed reperfusion. Quantitative PCR Western blot analysis were performed measure NOX2 expression. Reactive oxygen species (ROS) formation assessed dihydroethidium imaging superoxide tyrosine nitration. TUNEL...

Abstract Retinal ischemia is a major cause of visual impairment and blindness involved in various disorders including diabetic retinopathy, glaucoma, optic neuropathies retinopathy prematurity. Neurovascular degeneration common feature these pathologies. Our lab has previously reported that the ureahydrolase arginase 2 (A2) ischemic retinopathies. Here, we are introducing A2 as therapeutic target to prevent neurovascular injury after retinal ischemia/reperfusion (I/R) insult. Studies were...

Acute glaucoma is a leading cause of irreversible blindness in East Asia. The mechanisms underlying retinal neuronal injury induced by sudden rise intraocular pressure (IOP) remain obscure. Here we demonstrate that the activation CXCL10/CXCR3 axis, which mediates recruitment and inflammatory cells, has critical role mouse model acute glaucoma. mRNA protein expression levels CXCL10 CXCR3 were significantly increased after IOP-induced ischemia. Blockade pathway deleting gene attenuated...

We investigated the effect of tofogliflozin, a sodium-dependent glucose cotransporter 2 inhibitor (SGLT2i), on retinal blood flow dysregulation, neural dysfunction, and impaired neurovascular coupling in type diabetic mice. Tofogliflozin was added to mouse chow deliver 5 mg/kg/day 6-week-old mice were fed for 8 weeks. The longitudinal changes neuronal function responses systemic hyperoxia flicker stimulation evaluated every weeks db/db that received tofogliflozin (n =6) or placebo = 6) from...

Purpose: To show that noninvasive in vivo corneal confocal microscopy (IVCM) can make more accurate imaging of the subbasal nerve plexus possible. This diagnostic technique monitors status diabetic peripheral neuropathy. However, it is difficult to accurately confirm area captured by IVCM, which induce measurement errors. Because whorl-like characteristic pattern inferocentral cornea, we evaluated whether IVCM images patterns evaluate fibers Methods: Forty-seven patients with diabetes (DM...

Abstract We investigated and compared the susceptibility of retinal blood flow regulation neural function in mice developing type 2 diabetes. The longitudinal changes neuronal responses to a 10-min systemic hyperoxia 3-min flicker stimulation were evaluated every weeks diabetic db/db nondiabetic controls (db/m) from age 8 20 weeks. activity assessed using laser speckle flowgraphy electroretinography (ERG), respectively. had significantly higher glucose levels body weight. resting was steady...

We investigated the effect of fenofibrate nano-eyedrops (FenoNano) on impaired retinal blood flow regulation in type 2 diabetic mice. Six-week-old db/db mice were randomly divided into an untreated group (n = 6) and treated group, which received FenoNano 6). The longitudinal changes neuronal function responses to systemic hyperoxia flicker stimulation evaluated every weeks with or vehicle from ages 8-14 weeks. was assessed using laser speckle flowgraphy. also expressions vascular endothelial...

To investigate changes in choroidal blood flow (ChBF) the foveal region of human eye with rhegmatogenous retinal detachment induced by scleral buckling.ChBF was measured using laser Doppler flowmetry patients a and no macular involvement before after buckling. The ChBF ratio evaluated (ChBF affected to fellow control eye) minimise individual variations.Retinal reattachment confirmed 2 weeks buckling all patients. eyes did not differ from significantly (p<0.05) decreased 4 compared that...

Purpose.: To investigate the therapeutic use and mechanisms of action normobaric hyperoxia to promote revascularization prevent neovascularization in a mouse model oxygen-induced ischemic retinopathy. Methods.: Hyperoxia treatment (HT, 40%–75% oxygen) was initiated on postnatal day (P) 14 during pre-proliferative phase Immunohistochemistry, ELISA, quantitative PCR were used assess effects retinal vascular repair pathologic angiogenesis relation glial cell injury, VEGF protein, mRNA levels...

To investigate how neuronal nitric oxide synthase (nNOS) contributes to regulation of the retinal circulation during rest and flicker stimulation in cats.Using laser Doppler velocimetry, we measured vessel diameter blood velocity simultaneously calculated flow (RBF) feline first-order arterioles. After intravitreal injections Nω-Nitro-L-arginine methyl ester (L-NAME), a nonselective NOS inhibitor, Nω-propyl-L-arginine (L-NPA), selective nNOS continuously monitored without any perturbations...

Purpose: Because blinking is regarded as mechanical stimulation of fluid shear stress on the corneal epithelial cells, we investigated effects cultured human cells (HCECs). Methods: The HCECs were exposed to (0, 1.2, 12 dyne/cm2) with parallel-plate type flow chamber. Wound healing, cellular proliferation, growth factor expression, TGF-β1 concentration in culture supernatant, and phosphorylation SMAD2 investigated. Results: Monolayers had delayed wound healing decreased proliferation...

Abstract This study aimed to evaluate longitudinal changes in retinal blood flow response flicker stimulation and systemic hyperoxia mice using a laser speckle flowgraphy (LSFG-Micro). The vascular area surrounding the optic nerve head was measured 8-week-old male every 2 weeks until age 20-week. coefficient of variation under resting condition analyzed validate consistency measurement. On day 1 experiment, assessed 20 s for 6 min during after 3 light (12 Hz) stimulation; on 2, minute 10...

The aim was to determine whether serum prorenin levels affect the development of diabetic retinopathy (DR) in type 2 diabetes. Baseline were measured 196 patients (85 males, 111 females) with diabetes without DR using antibody-activating direct assay. fundi checked regularly. participants divided into two groups based on (high and low). We used Kaplan—Meyer analysis detect differences between within same gender. showed that males a high level tended develop earlier more frequently than low (...

We report a case of bilateral frosted branch angiitis (FBA) following mRNA-1273 COVID-19 vaccination. A 79-year-old male was referred to our hospital with sudden onset blurred vision in the right eye, which occurred during his return home after receiving third dose messenger RNA (mRNA) vaccine. Fundoscopy revealed severe retinal vasculitis sheathing artery and vein eye more so than left suggestive FBA. Optical coherence tomography showed significant macular edema serous detachment eye....

The visual outcome after vitrectomy for proliferative diabetic retinopathy (PDR) is often poor. Bilateral has been especially associated with a poor prognosis in patients PDR. authors investigated the systemic risk factors PDR requiring bilateral compared unilateral vitrectomy.The retrospectively reviewed 86 consecutive Type 2 diabetes mellitus who underwent vitrectomy. These were divided into groups: within 1 year (n = 25) and 61). factors: age, sex, duration of diabetes, hemoglobin A1c,...

We assessed the short-term effects of switching from intravitreal aflibercept (IVA) to faricimab (IVF) on ocular blood flow in patients with treatment-resistant diabetic macular edema (DME). The medical records 15 DME who had received IVA injection ≥ 3 months before were retrospectively reviewed. best-corrected visual acuity, central thickness (CMT) optical coherence tomography, and mean blur rate (MBR) all disc areas laser speckle flowgraphy measured before, 1 week after, 4 weeks after IVF,...

To examine the effects of hydrogen water on retinal blood flow (RBF) dysregulation in diabetes, we evaluated changes RBF response to flicker stimulation and systemic hyperoxia diabetic mice.