A5003854756- Heart Rate Variability and Autonomic Control
- Pulmonary Hypertension Research and Treatments
- Neuroscience of respiration and sleep
- Hydrogen's biological and therapeutic effects
- Nitric Oxide and Endothelin Effects
- Retinal Diseases and Treatments
- Advanced Chemical Sensor Technologies
- Retinal Imaging and Analysis
- Cardiovascular and exercise physiology
- Retinal and Optic Conditions
- Sleep and related disorders
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Renin-Angiotensin System Studies
- Heart Failure Treatment and Management
- Neuroinflammation and Neurodegeneration Mechanisms
- Biochemical effects in animals
- Behavioral Health and Interventions
- Eicosanoids and Hypertension Pharmacology
- Respiratory Support and Mechanisms
- Thermoregulation and physiological responses
- Hemodynamic Monitoring and Therapy
- Receptor Mechanisms and Signaling
- Analytical Chemistry and Sensors
- Hemoglobin structure and function
- Health and Wellbeing Research
National Cerebral and Cardiovascular Center
2008-2024
Chubu University
2017-2024
Asahikawa Medical University
2013-2023
Kyorin University
2023
Cardiovascular Research Center
2015
Osaka Medical and Pharmaceutical University
2001
Cardiovascular Institute Hospital
1997-2000
Pulmonary Vascular Research Institute
1997
Massachusetts General Hospital
1992-1993
Harvard University
1992-1993
cDNA Cloning of an EDRFINO Synthase were determined one these encoded amino acid sequence with significant homology to brain NOS.A restriction fragment prepared from this bovine was used screen a XGTlO library lipopolysaccharide-stimulated HUVEC (20).cDNA inserts hybridizing bacteriophage subcloned in pUC19.Nucleotide determination the longest performed by dideoxynucleotide chain termination method using nested deletions.Both sense and antisense DNA strands sequenced.Cloning Rat Gene...
We determined whether inhaling low levels of nitric oxide (NO) gas could selectively reverse hypoxic pulmonary vasoconstriction in the near-term newborn lamb and vasodilation would be attenuated by respiratory acidosis. To examine mechanism air NO-induced soon after birth, we measured plasma lung cGMP newly ventilated fetal lamb. Breathing at FIO2 0.10 nearly doubled vascular resistance index lambs decreased blood flow primarily reducing left-to-right through ductus arteriosus. Inhaling 20...
We hypothesized that exhaled acetone concentration (EAC), reflecting altered blood ketone body metabolism and increased exhaust because of pulmonary congestion in heart failure (HF), would correlate with hemodynamic parameters patients non-ischemic chronic HF.We prospectively enrolled 102 HF New York Heart Association (NYHA) class I-III. Exhaled breath was collected after an overnight fast. Echocardiography cardiac catheterization were performed all patients. also 17 control without HF. EAC...
To investigate the characteristics of intraretinal microvascular abnormalities (IRMAs) before and after panretinal photocoagulation (PRP) for diabetic retinopathy (DR) by using optical coherence tomography angiography (OCTA).Forty-six eyes 29 patients with DR were included (26 severe nonproliferative [SNPDR] 20 proliferative [PDR]). En face OCTA images IRMAs in a 6 × 6-mm area acquired Cirrus 5000 AngioPlex. The morphological changes evaluated PRP. divided into five subtypes: unchanged; tuft...
Purpose: The purpose of this study was to investigate the association between choriocapillaris microcirculation and visual function cone photoreceptor structure in patients with diabetes. Methods: Thirteen control subjects 26 type 2 diabetes were recruited. divided into three groups based on grade diabetic retinopathy (DR). retinal sensitivity (RS) evaluated using microperimetry. Cone mosaics imaged an adaptive optics camera, heterogeneity packing index (HPi) calculated. Optical coherence...
Rho-kinase-mediated vasoconstriction and endothelial dysfunction are considered two primary instigators of pulmonary arterial hypertension (PAH). However, their contribution to the adverse changes in blood flow distribution associated with PAH has not been addressed. This study utilizes synchrotron radiation microangiography assess specific role, of, PAH. Male adult Sprague-Dawley rats were injected saline (Cont-rats) or monocrotaline (MCT-rats) 3 wk before was performed on left lung. We...
Breath acetone is a noninvasive biomarker of heart failure; however, its significance in failure patients with diabetes mellitus has yet to be clarified. The objective this study investigate whether exhaled concentration mellitus.This prospectively included 35 diabetic stage C and 20 or at risk (stage A B). Exhaled breath was collected after an overnight fast.The group had significantly higher brain natriuretic peptide levels, larger left ventricular diameter, lower ejection fraction, more...
Abstract Sleep disturbances and cognitive decline are common in older adults. We aimed to investigate the effects of total sleep time (TST) sleep–wake rhythm on executive function working memory In 63 participants, we measured TST, wake after onset (WASO), timing (midpoint between bedtime wake-up time) using actigraphy. Executive was evaluated with trail making test B (TMT-B) Wisconsin card sorting (WCST). The number back task (N-back task) used measure memory. Participants a TST ≥ 8 h had...
Background —Patients with precapillary pulmonary hypertension (PH) exhibit a poor exercise capacity due to an impaired vasodilatory response of their arteries. By causing the artery dilate, inhaled nitric oxide (NO) may allow increase in patients PH. Methods and Results —On 2 separate days, 3 days apart, 14 PH (10 primary PH, 4 residual after correction intracardiac shunt; age, 40±12 years; mean pressure, 60±23 mm Hg) performed exercise, without inhalation 20 ppm NO, on cycle ergometer. The...
Endothelium-derived relaxing factor (EDRF)/nitric oxide (NO) activates soluble guanylate cyclase, thereby stimulating the synthesis of guanosine 3',5'-cyclic monophosphate (cGMP). To investigate regulation this important EDRF/NO receptor, we studied cyclase gene expression in a rat fetal lung fibroblast cell line (RFL-6). 3-Isobutyl-1-methylxanthine, forskolin, and dibutyryl adenosine (cAMP), agents which increase intracellular cAMP, decreased concentration mRNA encoding beta 1-subunit RFL-6...
Abstract Systemic inflammatory responses in patients receiving cardiac surgery with the use of cardiopulmonary bypass (CPB) significantly contribute to CPB‐associated morbidity and mortality. We hypothesized that insufflated hydrogen gas (H 2 ) would provide systemic anti‐inflammatory anti‐apoptotic effects during CPB, therefore reducing proinflammatory cytokine levels. In this study, we examined protective effect H on a rat CPB model. Rats were divided into three groups: sham operation...
Exhaled acetone concentration is one of the expected compounds to be a breath biomarker in heart failure. However, it has not been clarified how exhaled changes clinical course failure.To investigate whether after treatment acute decompensated failure (ADHF).This study included 19 patients with ADHF (ADHF group) and eight stable (control group). was collected from these patients, measured gas chromatography.The group had higher rates (P = 0.046), New York Heart Association class < 0.001),...
To compare how and to what extent ingestion of hydrogen water milk increase breath in adults.Five subjects without specific diseases, ingested distilled or as a reference material that could hydrogen. Their end-alveolar was measured.Ingestion rapidly increased the maximal level approximately 40 ppm 10-15 min after thereafter decreased baseline level, whereas same amount did not change (p < 0.001). Ingestion both peaks area under curve (AUC) dose-dependent manner. showed delayed sustained...
Chronic hypoxia (CH) increases lung tissue expression of all types nitric oxide synthase (NOS) in the rat. However, it remains unknown whether CH‐induced changes functional and histological NOS distributions are correlated rat small pulmonary arteries. We measured effects inhibitors on internal diameters (ID) muscular (MPA) elastic (EPA) arteries (100–700 μ m ID) using an X‐ray television system anaesthetized rats. also conducted immunohistochemical localization same vessels. Nonselective...
This study was conducted to determine adrenomedullin (AM) action sites in the pulmonary vascular bed and relation between its vasodilator effects tone. Moreover, an examination made into whether calcitonin gene‐related peptide (CGRP) receptors mediate vasodilatations induced by AM. To this end, we directly measured internal diameter (i.d.) changes small arteries veins (100–1100 μm i.d.) use of X‐ray televison system on vivo cat lung. Under control (resting tone) conditions, AM injections...