A5088905949- Mechanical Circulatory Support Devices
- Cardiac Ischemia and Reperfusion
- Cardiac Arrest and Resuscitation
- Cardiac and Coronary Surgery Techniques
- Cardiovascular Function and Risk Factors
- Cardiac Structural Anomalies and Repair
- Lipoproteins and Cardiovascular Health
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cancer, Lipids, and Metabolism
- Cardiac Imaging and Diagnostics
- Nitric Oxide and Endothelin Effects
- Respiratory Support and Mechanisms
- Neuroscience of respiration and sleep
- Pulmonary Hypertension Research and Treatments
- Adipose Tissue and Metabolism
- Cardiac electrophysiology and arrhythmias
- Anesthesia and Neurotoxicity Research
- Heart Rate Variability and Autonomic Control
- Cardiomyopathy and Myosin Studies
- Atherosclerosis and Cardiovascular Diseases
- Cardiovascular Health and Disease Prevention
- Congenital heart defects research
- Adipokines, Inflammation, and Metabolic Diseases
- Lipid metabolism and disorders
- Regulation of Appetite and Obesity
Niigata University of Health and Welfare
2017-2025
Miwa Hospital
2024
Hokkaido University Hospital
2024
Dokkyo Medical University
2024
National Cerebral and Cardiovascular Center
2011-2021
Tokyo Denki University
2018
Cardiovascular Institute Hospital
2012-2015
Osaka University
2012-2013
Kanazawa Municipal Hospital
2013
Kanazawa University Hospital
2013
In vitro expanded beating cardiac myocytes derived from induced pluripotent stem cells (iPSC-CMs) are a promising source of therapy for regeneration. Meanwhile, the cell sheet method has been shown to potentially maximize survival, functionality, and integration transplanted into heart. It is thus hypothesized that iPSC-CMs in manner may contribute functional recovery via direct mechanical effects on myocardial infarction (MI) F344/NJcl-rnu/rnu rats were left coronary artery ligated ( n =...
Venovenous extracorporeal membrane oxygenation (VV-ECMO) is an effective lung protection strategy that avoids ventilator-induced injury. However, appropriate respiratory settings for VV-ECMO are yet to be established. This study aimed elucidate the effects of ventilation under using a newly developed rat model and analyzed gene expression profiles. Rats were assigned three groups five rats each: spontaneous breathing, conventional-protective ventilation, ultra-protective ventilation. The...
Background: Cardiopulmonary bypass (CPB) and extracorporeal membrane oxygenation (ECMO) are widely used. Previous methods to reduce inflammation have shown inconsistent results. We developed a cytokine adsorption column using polymethyl methacrylate (PMMA) investigated its anti-inflammatory effects during ECMO. Materials Methods: Male Sprague–Dawley rats were divided into three groups (seven in each group): SHAM, ECMO, ECMO with PMMA (PMMA group). Experiments comprised 180 min of cannulation...
Cell-sheet transplantation induces angiogenesis for chronic myocardial infarction (MI), though insufficient capillary maturation and paucity of arteriogenesis may limit its therapeutic effects. Omentum has been used clinically to promote revascularization healing ischemic tissues. We hypothesized that cell-sheet covered with an omentum-flap would effectively establish mature blood vessels improve coronary microcirculation physiology, enhancing the effects therapy. Rats were divided into four...
Abstract Current therapeutic strategies for the treatment of critical limb ischemia (CLI) have only limited success. Recent in vitro evidence literature, using cell lines, proposes that peptide hormone ghrelin may angiogenic properties. In this study, we aim to investigate if could promote postischemic angiogenesis a mouse model CLI and, further, identify mechanistic pathway(s) underpin ghrelin's proangiogenic was induced male CD1 mice by femoral artery ligation. Animals were then randomized...
Activation of RhoA/Rho-kinase (ROCK) is increasingly implicated in acute vasospasm and chronic vasoconstriction major organ systems. Therefore we aimed to ascertain whether an increase ROCK activity plays a role the deterioration coronary vascular function early stage diabetes. Synchrotron radiation microangiography was used determine vivo responses diabetic (3 weeks post streptozotocin 65 mg/kg ip) vehicle treated male Sprague–Dawley rats (n = 8 6). Changes vessel number calibre during...
In diabetes, long-term micro- and macrovascular damage often underlies the functional decline in cardiovascular system. However, it remains unclear whether early-stage diabetes is associated with vivo impairment coronary microvasculature. Synchrotron imaging allows us to detect quantify regional differences resistance microvessel caliber vivo, even under conditions of high heart rate.Synchrotron cine-angiograms vasculature were recorded using anesthetized Sprague-Dawley rats 3 weeks after...
Abstract Systemic inflammatory responses in patients receiving cardiac surgery with the use of cardiopulmonary bypass (CPB) significantly contribute to CPB‐associated morbidity and mortality. We hypothesized that insufflated hydrogen gas (H 2 ) would provide systemic anti‐inflammatory anti‐apoptotic effects during CPB, therefore reducing proinflammatory cytokine levels. In this study, we examined protective effect H on a rat CPB model. Rats were divided into three groups: sham operation...
Systemic inflammatory responses in patients receiving cardiac surgery supported by cardiopulmonary bypass (CPB) significantly contribute to CPB-associated morbidity and mortality. We hypothesized that hyperoxia insufflation aggravates the organ damage during CPB. To verify this hypothesis, we investigated at high normal levels of arterial pressure oxygen (PaO2 ) rat CPB model. Rats were divided into a SHAM group, group > 400 mm Hg), normoxia : 100-150 Hg). measured serum cytokine tumor...
Structural and functional changes of pulmonary circulation related to pathophysiology arterial hypertension (PAH) remain be fully elucidated. Angiographic visualization in vivo animals provided a powerful tool for assessing the major indexes associated with pathogenesis PAH. In this study, we have exploited full potential synchrotron radiation (SR) microangiography show ability visualize hemodynamics closed-chest mouse. Male adult mice were anesthetized cannulated customized 24-gauge...
It has been shown that the size of myocardial infarction in rats created by coronary ligation technique is not uniform, varying from 4% to 65%. We hypothesized infarct variability induced artery might be caused branching pattern. Coronary angiography was performed 50 normal Lewis and chronic models which ligated immediately below left atrial appendage or 2mm distal (n = 25 for each), followed histological analysis. Unlike human, had a single major septal arising proximal part 30) right 20)....
Chronic intermittent hypoxia (IH) induces activation of the sympathoadrenal system, which plays a pivotal role in attenuating hypoxic pulmonary vasoconstriction (HPV) via central β1-adrenergic receptors (AR) (brain) and peripheral β2AR (pulmonary arteries). Prolonged hypercatecholemia has been shown to upregulate β3AR. However, relationship between IH β3AR modification HPV is unknown. It observed that chronic stimulation upregulates inducible nitric oxide synthase (iNOS) cardiomyocytes...
Chronic intermittent hypoxia (IH) induces oxidative stress and inflammation, which impair vascular endothelial function. Long-term insulin resistance also leads to dysfunction. We determined, in vivo, whether the effects of chronic IH on function augment each other. Male 12-wk-old Goto-Kakizaki (GK) Wistar control rats were subjected normoxia or (90-s N2, 5% O2 at nadir, 90-s air, 20 cycles/h, 8 h/day) for 4 wk. Coronary was assessed using microangiography with synchrotron radiation. Imaging...
Extracorporeal membrane oxygenation (ECMO) is being increasingly used for mechanical support of respiratory and cardio-circulatory failure. An excessive systemic inflammatory response observed during sepsis after cardiopulmonary bypass (CPB) with similar clinical features. We hypothesized that hyperoxia condition encourages the organ disorder ECMO. To prove this hypothesis correct, we investigated responses at normal high levels arterial oxygen pressure (PaO2) in rat ECMO model.Rats were...
In sleep apnea syndrome (SAS), intermittent hypoxia (IH) induces repeated episodes of hypoxic pulmonary vasoconstriction (HPV) during sleep, which presumably contribute to arterial hypertension (PAH). However, the prevalence PAH was low and severity is mostly mild in SAS patients, or no right ventricular hypertrophy (RVH) reported IH-exposed animals. The question then arises as why not a universal finding if sufficient duration causes cycling HPV. present study, rats underwent IH at rate 3...
Cardiopulmonary bypass (CPB) induced systemic inflammation significantly contributes to the development of postoperative complications, including respiratory failure, myocardial, renal and neurological dysfunction ultimately can lead failure multiple organs. Ghrelin is a small endogenous peptide with wide ranging physiological effects on metabolism cardiovascular regulation. Herein, we investigated protective ghrelin against CPB-induced inflammatory reactions, oxidative stress acute organ...
Impaired actin–myosin cross-bridge (CB) dynamics correlate with impaired left ventricular (LV) function in early diabetic cardiomyopathy (DCM). Elevated expression and activity of Rho kinase (ROCK) contributes to the development DCM. ROCK targets several sarcomeric proteins including myosin light chain 2, binding protein-C (MyBP-C), troponin I (TnI) T that all have important roles regulating CB contractility myocardium. Our aim was examine if chronic inhibition prevents LV dysfunction a rat...
Currently, nonpulsatile selective cerebral perfusion for cerebroprotection against thoracic aortic aneurysm is used in clinical settings. We performed synchrotron radiation microangiography to determine the effects on modulation by pulsatile flow. established at normothermia and severe hypothermia anesthetized rats, during which angiography was performed. NG-nitro-L-arginine-methyl ester hydrochloride (L-NAME) administered effect of flow with nitric oxide synthesis. In comparison flow,...