Chronic chagasic cardiomyopathy (CCC) develops years after acute infection by Trypanosoma cruzi and does not improve trypanocidal therapy, despite reduction of parasite burden. During disease, the heart undergoes oxidative stress, a potential causative factor for arrhythmias contractile dysfunction. Here we tested whether antioxidants/ cardioprotective drugs could cardiac function in established Chagas disease. We chose model that resembles B1-B2 stage human CCC, treated mice with...

CCL3, a member of the CC-chemokine family, has been associated with macrophage recruitment to heart tissue and parasite control in acute infection mouse Trypanosoma cruzi, causative agent Chagas disease. We approached participation CCL3 chronic chagasic cardiomyopathy (CCC), main clinical form induced CCC C57BL/6 (ccl3+/+) CCL3-deficient (ccl3-/-) mice by Colombian strain. In chronically infected ccl3+/+ mice, high levels mRNA protein were detected tissue. Survival was not affected...

In chronic schistosomiasis, hepatic fibrosis is linked to the portal hypertension that causes morbidity in Schistosoma mansoni infection. Silymarin (SIL) a hepatoprotective and antioxidant medicament largely prescribed against liver diseases has previously been shown prevent during acute murine schistosomiasis. Here we employed silymarin try reverse established or vehicle was administered BALB/c mice every 48 h, starting on 40th (80 days of treatment), 70th (50 days), 110th (10 days) day...

In chronic schistosomiasis, liver fibrosis is linked to portal hypertension, which a condition associated with high mortality and morbidity. High mobility group box 1 (HMGB1) was originally described as nuclear protein that functions structural co-factor in transcriptional regulation. However, HMGB1 can also be secreted into the extracellular milieu under appropriate signal stimulation. Extracellular acts multifunctional cytokine contributes infection, injury, inflammation, immune responses...

Chagasic chronic cardiomyopathy (CCC) is the primary clinical manifestation of Chagas disease (CD), caused by Trypanosoma cruzi. Current therapeutic options for CD are limited to benznidazole (Bz) and nifurtimox. Amiodarone (AMD) has emerged as most effective drug treating arrhythmic form CCC. To address effects Bz AMD we used a preclinical model Female C57BL/6 mice were infected with T. cruzi subjected oral treatment 30 consecutive days, either monotherapy or in combination. decreased...

Identifying new target molecules through which eosinophils secrete their stored proteins may reveal therapeutic approaches for the control of eosinophilic disorders such as host immune responses to parasites. We have recently reported expression purinergic P2Y12 receptor (P2Y12R) in human eosinophils; however, its functional role this cell type and involvement inflammation remain unknown. Here, we investigated roles P2Y12R isolated a murine model induced by Schistosoma mansoni (S. mansoni)...

Heme is a red blood cell component released in the brain parenchyma following intracerebral hemorrhage. However, study of pathophysiological mechanisms triggered by heme hampered lack well-established vivo models injection. This aims to optimize and characterize protocol intrastriatal injection mice, with focus on induction lipid peroxidation, neuroinflammation and, ultimately, sensorimotor deficits. We also evaluated involvement NLRP3 (NOD-, LRR-, pyrin domain-containing protein 3), an...

Anthelmintics used for intestinal helminthiasis treatment are generally effective; however, their effectiveness in tissue parasitosis (i.e. visceral toxocariasis) is moderate. The aim of this study was to evaluate the vitro activity lapachol, β-lapachone and phenazines relation viability Toxocara canis larvae. A concentration 2 mg/mL (in duplicate) compounds tested using microculture plates containing larvae an RPMI-1640 environment, incubated at 37 °C 5% CO2 tension 48 hours. In...

Memory impairment has been associated with chronic Chagas disease (CD), a neglected tropical caused by the protozoan parasite Trypanosoma cruzi . In degenerative diseases, memory loss increased oxidative stress, revealed as enhanced lipid peroxidation, in cerebral cortex. Benznidazole (Bz), trypanocidal drug efficient to reduce blood load acute and phases of infection, showed controversial effects on heart progression, main clinical manifestation CD. Here, we evaluated whether C57BL/6 mice...

Objective: We investigated the potential effects of oleic acid (OA) and glycerol monooleate (GMO) on skin delivery CXB.Methods: The influence both OA GMO (5.0% or 10.0%) in vitro permeability CXB (2.0%) was evaluated using propylene glycol (PG) as a vehicle. Also cytotoxicity genotoxicity vivo assays (skin irritation rabbits topical anti-inflammatory activity by mice) were conducted.Results: As expected, amount that permeated through minimal, but drug retention viable (epidermis plus dermis)...

The prevalence of Th1/Th2 response, spleen changes and megakaryocytes were investigated in BALB/c mice (n=138) infected with Leishmania infantum, treated infantum 30× (10-30) biotherapy - BioLi30×. We performed controlled experiments using 8-to-12-week-old mice, 5×107L. promastigotes, divided into eight groups: G1 (healthy), G2 (infected L. infantum), G3 (BioLi30× pre-treated), G4 pre/post-treated), G5 post-treated), G6 (Water G7 (Antimonium crudum post-treated) G8 (Glucantime®...

Chagas disease (CD), caused by the protozoan Trypanosoma cruzi , is a neglected tropical and health problem in Latin America. Etiological treatment has limited effectiveness chronic CD; thus, new therapeutic strategies are required. The practice of physical exercises been widely advocated to improve quality life CD patients. most frequent clinical manifestation indeterminate form (CIF), effect on progression remains unknown. Here, CIF model, we aimed evaluate cardiac histological,...

SUMMARY Human toxocarosis is a chronic tissue parasitosis most often caused by Toxocara canis. The seroprevalence can reach up to 50%, especially among children and adolescents. anthelmintics used in the treatment have moderate efficacy. aim of this study was evaluate vitro vivo anthelmintic activity quinones their derivatives against T. canis larvae cytotoxicity larvicidal compounds. compounds were evaluated at 1 mg mL −1 concentration microculture plates containing third stage an Roswell...

Silybin (SIB) is a hepatoprotective drug known for its poor oral bioavailability, attributed to classification as class IV with significant metabolism during the first-pass effect. This study explored potential of solid lipid nanoparticles (SLN-SIB-U) or without (SLN-SIB) ursodeoxycholic acid and polymeric (PN-SIB) delivery systems SIB. The efficacy these nanosystems was assessed through in vitro studies using GRX Caco-2 cell lines permeability proliferation assays, respectively, well vivo...

Background In Chagas disease (CD), a neglected tropical caused by the parasite Trypanosoma cruzi , development of mental disorders such as anxiety, depression, and memory loss may be underpinned social, psychological, biological stressors. Here, we investigated factors underlying behavioral changes in preclinical model CD. Methodology/Principal Findings T . -infected C57BL/6 mice, kinetic study (5 to 150 days postinfection, dpi) using standardized methods revealed sequential onset changes:...

Background: Celecoxib (CXB) has been explored as an anti-inflammatory or chemopreventive drug for topical treatment of skin diseases and cancer. Objective: The main aim this work was to investigate the potential dimethylsufoxide (DMSO) Azone (AZ) penetration enhancers (P.Es) delivery CXB. Method: in vitro studies, release, permeability cytotoxicity/genotoxicity were carried out with formulations containing not DMSO AZ (5% 10%). Skin irritation rabbits activity mice assayed vivo. Results:...

Oxidative stress promotes T . cruzi growth and development of chronic Chagas heart dysfunction. However, the literature contains gaps that must be fulfilled, largely due to variations in parasite DTU sources, cell types, mouse strains, tools manipulate redox status. We assessed impact oxidative environment on burden cardiomyoblasts effects Nrf2-inducer COPP function BALB/c mice infected with either DTU-II Y or DTU-I Colombian strains. Treatment antioxidants CoPP, apocynin, resveratrol,...

Long after Trypanosoma cruzi infection, 40% of individuals develop a progressive chronic chagasic cardiomyopathy (CCC), with systolic dysfunction and arrhythmias. Since we previously showed IL-1β mediates the development cardiac arrhythmias in diabetes mellitus cardiorenal syndrome, remains elevated Chagas disease patients, here tested role CCC using mouse model. Mice deficient IL-1R expression ( Il-1r −/− ) survived acute T. infection greater parasitemia than controls but did not lose...

Helminthes and enteric protozoa are serious problem of public health in developing countries. This study analyzed the prevalence intestinal parasites preschool children attend at basic unities (BHS) city Rio Grande, Grande do Sul, Brazil. Stool samples collected from 144 were analyzed, October 2009 to March 2010. The observed rate for enteroparasites was 17.4 %, including eggs nematodes Ascaris lumbricoides (11.1 %) Trichuris trichiura (6.3 %), cysts protozoan Giardia lamblia (4.9 %). Also,...

Background: 
 Leishmaniasis is a serious public health problem especially in developing countries [1]. The therapeutic potential of biotherapics against several microorganism has been described vitro [2,3] and vivo studies [4,5,6,7,8,9]. Considering the resistance leishmaniasis to conventional treatment as well previous with biotherapic, we evaluated effects Leishmania infantum 30x (BioLi30x) biotherapy.
 Aim: evaluate antileishmanial BioLi30x models.
 Methodology: experiments...