A5032315911- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Enzyme Catalysis and Immobilization
- SARS-CoV-2 detection and testing
- Markov Chains and Monte Carlo Methods
- Theoretical and Computational Physics
- COVID-19 epidemiological studies
- Blind Source Separation Techniques
- Peptidase Inhibition and Analysis
- Influenza Virus Research Studies
- Antimicrobial Resistance in Staphylococcus
- Cardiac Arrhythmias and Treatments
- Pancreatic function and diabetes
- Thyroid Disorders and Treatments
- Bayesian Modeling and Causal Inference
- Animal Virus Infections Studies
- Cell Image Analysis Techniques
- Conflict of Laws and Jurisdiction
- Single-cell and spatial transcriptomics
- Traumatic Brain Injury and Neurovascular Disturbances
- Machine Learning in Healthcare
- Enzyme function and inhibition
- Epigenetics and DNA Methylation
- Artificial Intelligence in Healthcare and Education
- Bacterial biofilms and quorum sensing
RWTH Aachen University
2023
Cologne Institute for Economic Research
2022-2023
PE International (Germany)
2023
Universitätsmedizin Greifswald
2018-2022
Asklepios Klinikum Harburg
2021-2022
German Centre for Cardiovascular Research
2022
Krankenhaus Reinbek - Startseite
2022
Asklepios Klinik Altona
2019-2021
Charité - Universitätsmedizin Berlin
2018
Triemli Hospital
2011
Abstract Certain hydrolases preferentially catalyze acyl transfer over hydrolysis in an aqueous environment. However, the molecular and structural reasons for this phenomenon are still unclear. Herein, we provide evidence that acyltransferase activity esterases highly correlates with hydrophobicity of substrate‐binding pocket. A scoring system developed work allows accurate prediction promiscuous solely from amino acid sequence cap domain. This concept was experimentally verified by...
Background The mRNA-based vaccine BNT162b2 of BioNTech/Pfizer has shown high efficacy against SARS-CoV-2 infection and a severe course the COVID-19 disease. However, little is known about long-term durability induced immune response resulting from vaccination. Methods In longitudinal observational study in employees at German hospital we compared humoral cellular 184 participants after two doses (BNT162b2) with mid-term follow-up 9 months. Anti-SARS-CoV-2 binding antibodies were determined...
Promiscuous acyltransferase activity is the ability of certain hydrolases to preferentially catalyze acyl transfer over hydrolysis, even in bulk water. However, poor enantioselectivity, low efficiency, significant product and limited substrate scope represent considerable drawbacks for their application. By activity-based screening several hydrolases, we identified family VIII carboxylesterase, EstCE1, as an unprecedentedly efficient acyltransferase. EstCE1 catalyzes irreversible amidation...
ObjectivesSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the pulmonary disease 2019 (COVID-19, which has challenged health care facilities worldwide. The sustainability of systems is largely reliant on status their workers (HCW).This study aimed to detect SARS-CoV-2 virus and specific antibodies among HCWs in a German hospital as model system for potential spread pandemic.MethodsBetween March June 2020, we used combination RT-PCR testing RNA an enzyme-linked...
Staphylococcus aureus is infamous for causing recurrent infections of the human respiratory tract. This a consequence its ability to adapt different niches, including intracellular milieu lung epithelial cells. To understand dynamic interplay between cells and pathogen, we dissected their interactions over 4 days by mass spectrometry. Additionally, investigated dynamics infection through live cell imaging, immunofluorescence electron microscopy. The results highlight major role often...
Background Since the introduction of various vaccines against SARS-CoV-2 at end 2020, infection rates have continued to climb worldwide. This led establishment a third dose vaccination in several countries, known as booster. To date, there has been little real-world data about immunological effect this strategy. Methods We compared humoral- and cellular immune response before after BioNTech/Pfizer vaccine BNT162b2, following different prime-boost regimen prospective observational study....
Abstract Certain hydrolases preferentially catalyze acyl transfer over hydrolysis in an aqueous environment. However, the molecular and structural reasons for this phenomenon are still unclear. Herein, we provide evidence that acyltransferase activity esterases highly correlates with hydrophobicity of substrate‐binding pocket. A scoring system developed work allows accurate prediction promiscuous solely from amino acid sequence cap domain. This concept was experimentally verified by...
Background: Implantable loop recorders (ILR) are a valuable tool for the investigation of unexplained syncopal episodes. The aim this retrospective single center study was to identify predictive factors pacemaker implantation in patients with syncope who underwent ILR insertion. Methods: One hundred six were retrospectively analyzed (mean age 59.1 years; 47.2% male) and negative conventional testing implantation. pri- mary endpoint detection symptomatic or asymptomatic bradycardia requiring...
In this work, we introduce an entirely data-driven and automated approach to reveal disease-associated biomarker risk factor networks from heterogeneous high-dimensional healthcare data. Our workflow is based on Bayesian networks, which are a popular tool for analyzing the interplay of biomarkers. Usually, data require extensive manual preprocessing dimension reduction allow effective learning networks. For data, hard automatize typically requires domain-specific prior knowledge. We here...
Abstract Objective Applications of machine learning in healthcare are high interest and have the potential to improve patient care. Yet, real-world accuracy these models clinical practice on different subpopulations remains unclear. To address important questions, we hosted a community challenge evaluate methods that predict outcomes. We focused prediction all-cause mortality as question. Materials Using Model-to-Data framework, 345 registered participants, coalescing into 25 independent...
The primary barrier that protects our lungs against infection by pathogens is a tightly sealed layer of epithelial cells. When the integrity this disrupted as consequence chronic pulmonary diseases or viral insults, bacterial will gain access to underlying tissues. A major pathogen can take advantage such conditions Staphylococcus aureus, thereby causing severe pneumonia. In study, we investigated how S. aureus responds different human epithelium, especially nonpolarization and fibrogenesis...
Little is known about the longevity of antibodies after a third dose mRNA-based SARS-CoV-2 vaccine BNT162b2 (BioNTech/Pfizer, Mainz, Germany). Therefore, serum antibody levels were evaluated in healthy adult healthcare workers Germany. These dropped significantly within short period 11 weeks from 4155.59 ± 2373.65 BAU/mL to 2389.10 1433.90 BAU/mL, p-value < 0.001 but remained higher than second (611.92 450.31 BAU/mL). To evaluate quality humoral immune response, we additionally measured...
We show nonparametric identification of the parameters in dynamic stochastic block model as recently introduced by Matias and Miele (2017) case binary, finitely weighted, general edge states. formulate conditions on true parameters, which guarantee actual point instead mere generic identification, also lead to novel conclusions static case. In particular, our results justify terms applications weighted edges with three give numerical illustrations via variational EM algorithm simulation...
Abstract Background Following a year of development, several vaccines have been approved to contain the global COVID-19 pandemic. Real world comparative data on immune response following vaccination or natural infection are rare. Methods We conducted longitudinal observational study in employees at secondary care hospital affected by Comparisons were made about presence anti-SARS-CoV-2 immunglobulin G (IgG) antibody ratio after infection, with one two doses BioNTech/Pfizer (BNT162b2), dose...
Abstract Introduction COVID-19, caused by SARS-CoV-2, is an occupational health risk especially for healthcare employees. This study was designed to determine the longitudinal seroprevalence of specific immunglobolin-G (IgG)-antibodies in employees a hospital setting. Methods All including and non-healthcare workers secondary care were invited participate this single-center study. After initial screening, 6 months follow-up done which included serological examination...
COVID-19, which is caused by SARS-CoV-2, an occupational health risk, especially for healthcare employees due to their higher exposure and consequently risk of symptomatic asymptomatic infections. This study was designed determine the longitudinal seroprevalence specific immunoglobulin-G (IgG) antibodies in a hospital setting. All secondary care hospital, including non-healthcare workers, were invited participate this single-center study. After initial screening, 6-month follow-up carried...
Multi-well plates and cell arrays enable microscopy-based screening assays in which many samples can be analysed parallel. Each of the formats possesses its own strengths weaknesses, but reference comparisons between these platforms their application rationale is lacking. We aim to fill this gap by comparing two RNA interference (RNAi)-mediated fluorescence assays, namely epidermal growth factor (EGF) internalization cycle progression, on both platforms. Quantitative analysis revealed that...
Background Approaching epidemiological data with flexible machine learning algorithms is of great value for understanding disease-specific association patterns. However, it can be difficult to correctly extract and understand those patterns due the lack model interpretability. Method We here propose a workflow that combines random forests Bayesian network surrogate models allow deeper level interpretation complex first evaluate proposed on synthetic data. then apply from large...
Zusammenfassung Wie schon die Hochwasserereignisse 2002 und 2013 haben auch Folgen der Flutkatastrophe im Juli 2021 dramatisch Notwendigkeit von gesellschaftlichen Klimaanpassungsstrategien in Deutschland aufgezeigt. Bund Länder zum dritten Mal eine Arbeitsgruppe mit verfassungsrechtlichen Prüfung einer Versicherungspflicht gegen Elementarschäden beauftragt. Während sich grundsätzlichen Argumente Debatte um Einführung wiederholen, lohnt ein Blick auf zentralen Ausgestaltungsfragen...
Total knee arthroplasty (TKA) patients may present with genetic deformities, such as trochlear dysplasia, or deformities related to osteoarthritis. This pathologic morphology should be corrected by TKA compensate for functional deficiencies. Hence, a reconstruction of an equivalent physiological would favorable detailed preoperative planning and the patient-specific implant selection design process. A parametric database 673 knees, each described 36 femoral parameter values, was used. Each...
Abstract In this paper, we discuss whether Germany fulfills its international commitments in financing climate projects vulnerable countries of the Global South or is rather guided by German interests. To end, take a critical look at fact that are mainly financed through development cooperation budget. We also allocation funds between change adaptation and mitigation. argue budget should be increased, finance focus more on adaptation.
Measures of algorithmic fairness are usually discussed in the context binary decisions. We extend approach to continuous scores. So far, ROC-based measures have mainly been suggested for this purpose. Other existing methods depend heavily on distribution scores, unsuitable ranking tasks, or their effect sizes not interpretable. Here, we propose a distributionally invariant version scores with reasonable interpretation based Wasserstein distance. Our easily computable and well suited...