A5102021334- IL-33, ST2, and ILC Pathways
- Asthma and respiratory diseases
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Neonatal Respiratory Health Research
- RNA modifications and cancer
- Pediatric health and respiratory diseases
- RNA Research and Splicing
- Air Quality and Health Impacts
- Extracellular vesicles in disease
- Fungal Infections and Studies
- Epigenetics and DNA Methylation
- Eosinophilic Esophagitis
- S100 Proteins and Annexins
- Inhalation and Respiratory Drug Delivery
- Peroxisome Proliferator-Activated Receptors
- Immune responses and vaccinations
- Pleural and Pulmonary Diseases
- Antimicrobial Peptides and Activities
- Antifungal resistance and susceptibility
- Retinoids in leukemia and cellular processes
- Air Quality Monitoring and Forecasting
- Eosinophilic Disorders and Syndromes
Nanjing Medical University
2018-2024
Jiangsu Province Hospital
2018-2024
Northwest A&F University
2009-2010
Epithelial-mesenchymal transition (EMT) is essential in asthma airway remodeling. IL-33 from epithelial cells involved pulmonary fibrosis. CD146 has been extensively explored cancer-associated EMT. Whether regulates the EMT process associated with remodeling still largely unknown. We hypothesized that was regulated by IL-33/CD146 axis. House dust mite (HDM) extract increased expression of and cells. Increased HDM-treated could be blocked an ST2-neutralizing antibody. Moreover, HDM-induced...
The roles and mechanisms of long noncoding RNAs (lncRNAs) in T helper 2 (Th2) differentiation from allergic asthma are poorly understood. We aimed to explore a novel lncRNA, LincR-protein phosphatase regulatory subunit B' gamma (PPP2R5C), Th2 mouse model asthma.
Proline and its synthesis enzyme pyrroline-5-carboxylate reductase 1 (PYCR1) are implicated in epithelial-mesenchymal transition (EMT), yet how proline PYCR1 function allergic asthmatic airway remodeling via EMT has not been addressed. In the present study, increased levels of plasma were observed patients. Similarly, lung tissues higher a murine asthma model induced by house dust mites (HDMs). Pycr1 knockout (KO) decreased tissues, with reduced EMT. Mechanistically, loss restrained...
Accumulating evidence indicates that fine particulate matter (PM2.5) exposure is associated with many cardiopulmonary diseases, particularly lung carcinoma. Nevertheless, the underlying biological mechanisms by which PM2.5 initiates and aggravates carcinoma remain elusive. In present study, we collected in Nanjing explored oncogenic roles of murine cell line LLC vitro vivo. was closely attached to internalized cancer cells. Moreover, increased production ARNT2 inactivation tumor suppressor...
A further examination of a novel miRNA,miR-1165-3p as biomarker for asthma, which was previously implicated in helper T cells (Th2) murine asthma model.To determine whether serum miR-1165-3p can serve potential diagnostic allergic asthma.Serum quantified via quantitative real-time PCR (qRT-PCR) asthmatic and control samples. Serum levels were compared between groups the clinical abilities evaluated. The analyses utilized included student's t test, one-way ANOVA, generation receiver operating...
CD4⁺T cells are essential in the pathogenesis of allergic asthma. We have previously demonstrated that microRNA-1165-3p (miR-1165-3p) was significantly reduced T-helper type (Th) 2 and miR-1165-3p a surrogate marker for atopic Little is known about mechanisms regulation Th2-dominated inflammation. aimed to investigate associations between Th2 differentiation miR-1165b-3p asthma as well possible mechanisms.CD4⁺ naïve T were differentiated into Th1 or vitro. MiR-1165-3p up-regulated...
Although long non-coding RNAs (lncRNAs) have been linked to many diseases including asthma, little is known about lncRNA transcriptomes of CD4+ T cells in asthma. The present study aimed explore the lncRNAs profile CD4+T from mouse model acute asthma.Next generation sequencing for and mRNAs was performed on asthma control mice. Gene ontology (GO) kyoto encyclopedia genes genomes (KEGG) pathway analyses were predict functions signal pathways aberrant lncRNAs. selected further measured using...
Pulmonary edema is a fatal complication of EV71-associated hand, foot, and mouth disease (HFMD). The pathogenesis EV71-induced pulmonary remains largely unclear. In this study, we aimed to explore the roles capsid protein VP1 in occurrence edema. intranasal inoculation recombinant caused lung inflammation with an elevation inflammatory cytokines neutrophils infiltration. Moreover, neutrophil extracellular traps (NETs) were observed parenchyma mice treated VP1. directly induced formation...
The IL-33/ST2 axis has been extensively investigated in type 2 eosinophilic inflammation. Here, we aimed to investigate the role of neutrophil-dominant allergic airway inflammation.House-dust mite (HDM) extract and lipopolysaccharide (LPS) were administered establish a murine model formation neutrophilic extracellular traps (NETs) lung tissues was demonstrated by immunofluorescence imaging. Mature IL-33 bronchoalveolar lavage fluid (BALF) detected Western blotting. chemokine KC produced bone...
So far, epidemiological data have revealed that the elevation of fine ambient particulate matter (aerodynamic diameter ≤2.5 μm; PM2.5) is closely associated with an exacerbation asthma while underlying mechanism poorly understood. Using a murine model, we investigated impact PM2.5 on development asthma. Before OVA challenge, mice were administrated PM2.5, phosphate-buffer saline (PBS) or control filter extracts (CFE). Results showed compared to PBS CSF, co-exposure led higher airway...
Abstract This study examined the levels of soluble CD146 (sCD146) in plasma samples from patients with chronic obstructive pulmonary disease (COPD) and assessed relationship between sCD146 severity COPD. A total 97 COPD were recruited 20 medical centers Jiangsu, China, including 13 stable subjects 84 exacerbated subjects. The level (28.77 ± 10.80 ng/mL) was significantly lower than that (38.84 15.00 ng/mL). In high group, proportion modified Medical Research Council (mMRC) scores 0–1 higher,...
Switching of airway smooth muscle (ASM) cell phenotype from differentiated-contractile to dedifferentiated-proliferative/synthetic state often occurs in asthmatic subjects with dysfunction. Evidence has been provided that chloroquine (an agonist bitter taste receptors) presented benefits ASM function implicated asthma. However, the underlying mechanism is unclear. House dust mite (HDM)-sensitized mice were administered or dexamethasone before challenge. BALF and lung tissue obtained for...
Air pollution is a leading cause of increasing infectious lung diseases. Pulmonary cryptococcosis fatal fungal pneumonia in acquired immunodeficiency syndrome patients. In some cases, the pathogen Cryptococcus neoformans also develops dormant nodules immunocompetent individuals. present study, we demonstrated that fine particulate matter (PM 2 . 5 ) increased CD146 expression alveolar epithelial cells and promoted C. pulmonary infection. Aryl hydrocarbon receptor (AhR) signaling was required...
Malignant pleural effusion (MPE) is a complicated condition of patients with advanced tumors. Further dissecting the microenvironment infiltrated immune cells and malignant are warranted to understand immune-evasion mechanisms tumor development progression.
CD3+CD4-CD8- double-negative T (DNT) cells are new weapons in cancer immunotherapy. Here, we explored DNT malignant pleural effusions (MPEs) from lung patients. cells, especially TCRαβ+CD56- were increased MPE highly expressed PD-1, TRAIL, NKG2D and DNAM-1. In contrast, FasL was barely detected cells. Compared with non-MPE MPE-derived much higher levels of PD-1 TRAIL. healthy peripheral blood donors potentially killed cancers, which decreased by supernatant. Exosomes supernatant CEACAM1...
To explore the miRNAs profile of CD4+ T lymphocytes in asthma via next generation sequencing. In murine model acute asthma, spleen were sorted, which small RNAs extracted and sequenced. Novel measured with real time quantitative reverse transcription polymerase chain reaction (qRT-PCR). A total 127 found to exhibit at least twofold change. 262 predicted novel miRNAs, 14 qRT-PCR sorted cells or differentiated Th1/Th2 miR-11 (xxx-m0228-3p) was significantly decreased from Th2 cells. Aberrant...
Abstract: Organizing pneumonia (OP) is a distinct clinical and pathologic entity. This condition can be cryptogenic (COP) or secondary to other known causes (secondary OP, SOP). Concomitant occurrence of invasive pulmonary aspergillosis (IPA) with SOP unusual. Here, we report case where was presenting feature in patient diagnosed IPA. A previously healthy 62-year-old man presented the hospital month intermittent fever accompanied by cough expectoration. According computed tomography (CT),...
Abstract Background The microtubule-dependent molecular motor protein Kinesin Family Member 2A (KIF2A) is down-regulated in asthmatic human airway epithelium. However, little known about the roles of KIF2A as well possible underlying mechanisms asthma. Methods House dust mite (HDM) extract was administered to establish a murine model expression KIF2A, IL-33 and autophagy pathways were detected. plasmid pCMV-KIF2A used overexpress epithelial cells vitro vivo. IL-4, IL-5, other cytokines...
Allergic asthma is largely dominated by Th2 lymphocytes. Micropeptides in cells and remain unmasked. Here, we aimed to demonstrate a micropeptide, T-cell regulatory micropeptide (TREMP), cell differentiation asthma.
Airway remodeling is a key characteristic of allergic asthma. Epithelial-mesenchymal transition (EMT) induced by various factors, particularly transforming growth factor (TGF)-β1, orchestrates airway remodeling. Protein phosphatase 2A (PP2A), an important serine-threonine phosphatase, involved in TGF-β1 production and EMT. Long noncoding RNAs (lncRNAs) have emerged as novel players regulating Here, we aimed to explore the effects mechanisms action lincR-PPP2R5C, lncRNA that affects PP2A...