A5041917988- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive System and Pregnancy
- Ovarian function and disorders
- Pluripotent Stem Cells Research
- Endometriosis Research and Treatment
- Estrogen and related hormone effects
- Renal and related cancers
- Reproductive Physiology in Livestock
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Assisted Reproductive Technology and Twin Pregnancy
- Bioactive Compounds and Antitumor Agents
- Pregnancy and preeclampsia studies
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Gynecological conditions and treatments
- Prenatal Screening and Diagnostics
- Reproductive Health and Technologies
- Bee Products Chemical Analysis
- Ectopic Pregnancy Diagnosis and Management
- COVID-19 Impact on Reproduction
- Sexual Differentiation and Disorders
- Heat shock proteins research
- 3D Printing in Biomedical Research
- Ferrocene Chemistry and Applications
National Hospital
2015-2024
P. D. Hinduja Hospital and Medical Research Centre
2014-2024
Christie's
2022
Jaslok Hospital
2005-2018
University of Mumbai
2016
Breach Candy Hospital
2015
E Ink (South Korea)
2011-2013
National Institute for Research in Reproductive Health
1993
Bombay Hospital
1989-1991
College of Medicine & JNM Hospital
1985-1991
The present study was undertaken to detect, characterize, and differentiation potential of stem cells in adult rabbit, sheep, monkey, menopausal human ovarian surface epithelium (OSE). Two distinct populations putative (PSCs) variable size were detected scraped OSE, one being smaller other similar the surrounding red blood OSE. 1-3 μm very small embryonic-like PSCs pluripotent nature with nuclear Oct-4 cell SSEA-4, whereas bigger 4-7 cytoplasmic localization minimal expression SSEA-4...
Polycystic ovary syndrome (PCOS), a major cause of anovulatory infertility, is characterized by arrested follicular growth. Altered protein levels in the fluid surrounding ovum may reflect molecular defects folliculogenesis these women.To identify differentially regulated proteins PCOS comparing repertoire with healthy women.The samples were collected from and normo-ovulatory women undergoing vitro fertilization. Follicular subjected to digestion using trypsin, resultant peptides labeled...
Recent studies suggest that ovarian germ line stem cells replenish oocyte-pool in adult stage, and challenge the central doctrine of 'fixed cell pool' mammalian reproductive biology. Two distinct populations spherical with high nucleo-cytoplasmic ratio have been recently identified ovary surface epithelium (OSE) including nuclear OCT-4A positive very small embryonic-like (VSELs) cytoplasmic OCT-4 expressing (OGSCs). Three weeks culture scraped OSE results spontaneous differentiation into...
Problem Implantation failure (IF) even after the good‐quality embryo transfer (ET) is main obstacle in vitro fertilization (IVF). We aim to study genomics of endometrial receptivity IF patients under controlled ovarian stimulation (COS) during which ET generally practised IVF. Method Endometrial gene expression profiling (n=10) and oocyte donors (n=8) were compared window implantation COS by microarray. Enrichment analysis microarray data was performed determine dysregulated pathways....
The urge to have one's own biological child supersedes any desire in life. Several options been used obtain gametes including pluripotent stem cells (embryonic ES and induced iPS cells); gonadal (spermatogonial SSCs, ovarian OSCs cells), bone marrow, mesenchymal fetal skin. However, the field poses a huge challenge inefficient existing protocols for differentiation, epigenetic genetic changes associated with extensive vitro manipulation also ethical/regulatory constraints. A tremendous leap...
This study describes the successful derivation of two human embryonic stem (hES) cell lines using 53 frozen and 18 fresh “slow-growing” surplus embryos, obtained from collaborating in vitro fertilization clinics, on in-house–derived feeder layers. The have been derived by whole embryo culture followed further expansion manually dissected inner mass surrounding trophoectodermal cells. Immunocytochemical localization surface markers like SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, staining for...
Is angiogenic potential of follicular fluid (FF) and granulosa-lutein cells (GLCs) altered in polycystic ovary syndrome (PCOS) does it play a role corpus luteum (CL) defect observed them?FF GLCs women with PCOS show reduced expression pro-angiogenic factors compared to controls exhibit diminished capacity induce angiogenesis.In PCOS, CL insufficiency frequent miscarriage are reported, which may be due CL. The development new blood vessels is essential promote ovarian folliculogenesis...
Spermatozoan nuclear chromatin is in a highly condensed state prior to fertilization. In vivo decondensation occurs the ooplasm and essential for successful fertilization formation of male pronucleus zygote occur. The spermatozoa nucleus can undergo vitro with sodium dodecyl sulfate (SDS) 6 mM ethylene diamine tetraacetic acid (EDTA). ability sperm decondense was compared their fertilize human oocytes vitro. Spermatozoa from normal samples were studied as regards fertilizing performance an...
Polycystic ovary syndrome (PCOS) is a complex endocrinopathy affecting women of reproductive age, and whose etiology not well understood yet. In these women, the follicular growth arrested at preantral stage leading to cyst formation, consequently resulting in anovulatory infertility women. As fluid provides conducive microenvironment for oocytes, molecular profiling may provide unique information about pathophysiology associated with development PCOS. Post-translational addition...
The centrosome is the microtubule organizing center (MTOC) paternally inherited by zygote during fertilization. As located in midpiece of sperm tail, we presume that oligoasthenozoospermic samples should also have abnormal concentrations centrosomal proteins. This study therefore aims to determine if there any correlation between proteins, centrin, α and γ-tubulin, from normozoospermic men.
Abstract Problem DNA methylation profile in mid‐secretory phase of endometrium is reported to be varied from other phases natural menstrual cycle. Therefore, we intended study the impairment endometrial receptivity by performing whole‐genome and gene expression profiling recurrent implantation failure patients (RIF) during IVF under controlled ovarian stimulation (COS). Method Endometrial biopsies were collected IVF‐RIF (cases, n = 6) healthy fertile oocyte donors (controls, undergoing COS...