A5086717421- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Renal and related cancers
- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- FOXO transcription factor regulation
- Pancreatic function and diabetes
- Glycosylation and Glycoproteins Research
- Ovarian function and disorders
- Cancer-related Molecular Pathways
- Prostate Cancer Treatment and Research
- Sperm and Testicular Function
- Phytochemicals and Medicinal Plants
- Cancer, Hypoxia, and Metabolism
- Hippo pathway signaling and YAP/TAZ
- Neuroendocrine Tumor Research Advances
- Metabolism, Diabetes, and Cancer
- Microtubule and mitosis dynamics
- Animal Genetics and Reproduction
- Phosphodiesterase function and regulation
- Fungal Biology and Applications
- interferon and immune responses
- Cancer, Lipids, and Metabolism
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
University of Nebraska Medical Center
2020-2025
National Institute for Research in Reproductive Health
2011-2023
University of Nebraska at Omaha
2020-2021
University of Louisville
2016-2020
James Graham Brown Foundation
2017-2020
Indian Council of Medical Research
2014-2016
The present study was undertaken to detect, characterize, and differentiation potential of stem cells in adult rabbit, sheep, monkey, menopausal human ovarian surface epithelium (OSE). Two distinct populations putative (PSCs) variable size were detected scraped OSE, one being smaller other similar the surrounding red blood OSE. 1-3 μm very small embryonic-like PSCs pluripotent nature with nuclear Oct-4 cell SSEA-4, whereas bigger 4-7 cytoplasmic localization minimal expression SSEA-4...
Abstract Background We have earlier reported that follicle stimulating hormone (FSH) modulates ovarian stem cells which include pluripotent, very small embryonic-like (VSELs) and their immediate descendants ‘progenitors’ termed germ (OGSCs), lodged in adult mammalian surface epithelium (OSE). FSH may exert pleiotropic actions through its alternatively spliced receptor isoforms. Four isoforms of receptors (FSHR) are literature FSH-R1 FSH-R3 biological activity. Present study was undertaken to...
Ovarian cancer is a complicated malady associated with stem cells (CSCs) contributing to 238,700 estimated new cases and 151,900 deaths per year, worldwide. CSCs comprise tiny fraction of tumor-bulk responsible for recurrence eventual mortality. or tumor initiating are self-renewal, differentiation proliferative potential, initiation capability, its progression, drug resistance metastatic spread. Although several biomarkers implicated in these processes, their distribution within the ovary...
Recent studies suggest that ovarian germ line stem cells replenish oocyte-pool in adult stage, and challenge the central doctrine of 'fixed cell pool' mammalian reproductive biology. Two distinct populations spherical with high nucleo-cytoplasmic ratio have been recently identified ovary surface epithelium (OSE) including nuclear OCT-4A positive very small embryonic-like (VSELs) cytoplasmic OCT-4 expressing (OGSCs). Three weeks culture scraped OSE results spontaneous differentiation into...
Abstract Background Cryopreserved ovarian cortical tissue acts as a source of primordial follicles (PF) which can either be auto-transplanted or cultured in vitro to obtain mature oocytes. This offers good opportunity attain biological parenthood individuals with gonadal insufficiency including cancer survivors. However, role various intra- and extra-ovarian factors during PF growth initiation still remain poorly understood. Ovarian biology has assumed different dimension due emerging data...
Abstract Background Stem cells in the ovary comprise of two distinct populations including very small embryonic-like stem (VSELs) and slightly bigger progenitors termed ovarian (OSCs). They are lodged surface epithelium (OSE) expected to undergo neo-oogenesis primordial follicle (PF) assembly adult ovaries. The express stimulating hormone (FSH) receptors directly activated by FSH resulting formation germ cell nests (GCN) vitro. Present study was undertaken further characterize sheep OSCs...
// Sham S. Kakar 1, 2 , Seema Parte Kelsey Carter 1 Irving G. Joshua Christopher Worth Pranela Rameshwar 3 and Mariusz Z. Ratajczak 4 Department of Physiology, University Louisville, KY 40202, USA James Graham Brown Cancer Center, Medicine, Hematology/Oncology, Rutgers, New Jersey Medical School, Newark, NJ 07103, Correspondence to: Kakar, email: sskaka01@louisville.edu Keywords: cancer stem cells, ALDH1, ovarian cancer, withaferin A, securin Received: May 09, 2017    ...
Docetaxel plays an indispensable role in the management of advanced prostate cancer. However, more than half patients do not respond to docetaxel, and those good responders frequently experience significant cumulative toxicity, which limits its dose duration intensity. Hence, a second agent that could increase initial efficacy docetaxel maintain tolerability at biologically effective doses may improve outcomes for patients.We determined phosphodiesterase 5 (PDE5) expression levels human...
Lei and Spradling in a recent study published PNAS failed to detect 'germline cysts' by elegant studies using lineage tracing approach thus concluded that adult mouse ovaries lack stem cells. They proposed primordial follicle pool generated during fetal life is sufficient sustain oogenesis there no renewal of oocytes life. Contrary their results, we have reported presence very small pluripotent, embryonic-like cells (VSELs), immediate descendants (OGSCs) germ cell 'cysts' or 'nests' (formed...
The spontaneous return of fertility after bone marrow transplantation or heterotopic grafting cryopreserved ovarian cortical tissue has surprised many, and a possible link with stem cells been proposed. We have reviewed the available literature on in adult mammalian ovaries presented model that proposes ovary harbors two distinct populations cells, namely, pluripotent, quiescent, very small embryonic-like (VSELs), slightly larger “progenitor” germ (OGSCs). Besides compromising somatic niche,...
Cisplatin- and gemcitabine-based chemotherapeutics represent a mainstay of cancer therapy for most solid tumors; however, resistance limits their curative potential. Here, we identify RNA polymerase II-associated factor 1 (PAF1) as common driver cisplatin gemcitabine in human cancers (ovarian, lung, pancreas). Mechanistically, cisplatin- gemcitabine-resistant cells show enhanced DNA repair, which is inhibited by PAF1 silencing. We demonstrate an increased interaction with RAD52 resistant...
Ovarian cancer is most lethal among gynecological cancers with often fatal consequences due to lack of effective biomarkers and relapse, which propels ovarian research into unique directions establish solid targeted therapeutics. "Ovarian stem cells" expressing germline pluripotent markers serve as novel paradigm potential address infertility, menopause, probably influence tumor initiation. Cancer cells (CSCs) pose vital role in recurrence hence it extremely important study them respect...