A5045098159- Dermatology and Skin Diseases
- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Lung Cancer Treatments and Mutations
- Nitric Oxide and Endothelin Effects
- Urticaria and Related Conditions
- Mast cells and histamine
- Lung Cancer Research Studies
- Food Allergy and Anaphylaxis Research
- Colorectal Cancer Treatments and Studies
- Microbial Community Ecology and Physiology
- Exercise and Physiological Responses
- Cardiac Fibrosis and Remodeling
- Hormonal Regulation and Hypertension
- Ion channel regulation and function
- Peptidase Inhibition and Analysis
- Cardiac Imaging and Diagnostics
- Cardiovascular Disease and Adiposity
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Medical Imaging and Pathology Studies
- Immune Cell Function and Interaction
- Pharmacological Effects of Natural Compounds
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Ion Channels and Receptors
- Heart Failure Treatment and Management
Okayama University Hospital
2009-2025
Beth Israel Deaconess Medical Center
2016-2024
Kyoto Pharmaceutical University
2014-2024
Meijo University
2024
Harvard University
2016-2023
Kitami Institute of Technology
2021-2022
The University of Tokyo
2014-2021
National Cancer Center Hospital East
2020-2021
Serdang Hospital
2021
Okayama University
2007-2020
The EGFR-A763_Y764insFQEA is a unique exon 20 insertion mutation (~5% to 6% of insertions), which, at the structural and enzyme kinetic level, more closely resembles EGFR tyrosine kinase inhibitor (TKI)- sensitizing mutants, such as 19 indels L858R. A limited number preclinical models clinical reports have studied response this mutant TKIs.We used typical mutations probe representative first- (gefitinib, erlotinib), second- (afatinib), third-generation (osimertinib), in-development...
Tumor heterogeneity underlies resistance to tyrosine kinase inhibitors (TKI) in lung cancers harboring EGFR mutations. Previous evidence suggested that subsets of preexisting resistant cells are selected by EGFR-TKI treatment, or alternatively, diverse acquired mechanisms emerge from drug-tolerant persister (DTP) cells. Many studies have used bulk tumor specimens subcloned cell lines identify mechanism. However, intratumoral can result divergent responses therapies, requiring additional...
Abstract Amplification and/or overexpression of human epidermal growth factor receptor 2 (HER2) are observed in 15–20% breast cancers (HER2+ cancers), and anti-HER2 therapies have significantly improved prognosis patients with HER2+ cancer. One resistance mechanism to is constitutive activation the phosphoinositide 3-kinase (PI3K) pathway. Combination therapy small-molecule inhibitors AKT HER2 was conducted cancer cell lines or without PIK3CA mutations, which lead PI3K mutations played...
"Red snow" refers to red-colored snow, caused by bloom of cold-adapted phototrophs, so-called snow algae. The red found in Langhovde, Antarctica, was investigated from several viewpoints. Various sizes rounded cells were observed the samples under microscopy. Pigment analysis demonstrated accumulation astaxanthin snow. Community structure microorganisms analyzed culture-independent methods. In analyses small subunit rRNA genes, species green algae, fungus, and various phylotypes bacteria...
Malignant pleural mesothelioma (MPM) is an aggressive and therapy-resistant neoplasm arising from the mesothelial cells, without established indicators to predict responsiveness chemotherapy.Our study involving 79 MPM patients showed that 73.4% of expressed CD26 on cell membrane.The majority epithelioid biphasic types membrane, whereas sarcomatoid type a lack surface expression. Although was associated with poor prognosis (P < 0.0001), no significant relationship between expression survival...
Histone modifications play crucial roles in transcriptional activation, and aberrant epigenetic changes are associated with oncogenesis. Lysine (K) acetyltransferases 5 (TIP60, also known as KAT5) is reportedly implicated cancer development maintenance, although its function lung remains controversial. Here we demonstrate that TIP60 knockdown non-small cell lines decreased tumor growth, migration, invasion. Furthermore, analysis of a mouse model lung-specific conditional Tip60 knockout...
Abstract Hairless mice fed a special diet, HR ‐ AD , develop atopic dermatitis ( )‐like skin inflammation with barrier defects and itch‐related scratching; however, the ingredient(s) causing remains unclear. In this study, we examined whether deficiency of certain polyunsaturated fatty acids PUFA s) is involved in ‐induced . High‐purity s were given to ‐fed by dietary supplementation or gavage. Fatty acid levels serum determined using gas chromatography–mass spectrometry. from mice, linoleic...
Malignant pleural mesothelioma (MPM) usually develops fluid. We investigated the value of DNA methylation in fluid for differentiating MPM from lung cancer (LC). Pleural was collected 39 patients with MPM, 46 LC, 25 benign asbestos pleurisy (BAP) and 30 other causes. The O(6)-methylguanine-DNA methyltransferase (MGMT), p16(INK4a) , ras association domain family 1A (RASSF1A), death-associated protein kinase (DAPK), retinoic acid receptor β (RARβ) examined using quantitative real-time PCR....
The Ca<sup>2+</sup>-activated Cl<sup>−</sup> channel transmembrane proteins with unknown function 16 A (TMEM16A; also known as anoctamin 1 or discovered on gastrointestinal stromal tumor 1) plays an important role in facilitating the cell growth and metastasis of TMEM16A-expressing cancer cells. Histone deacetylase (HDAC) inhibitors (HDACi) are useful agents for therapy, but it remains unclear whether ion channels epigenetically regulated by them. Using real-time polymerase chain reaction,...
Mast cell hyperplasia has been observed in the lungs of mice with experimental asthma, but few reports have studied basophils. Here, we attempted to discriminate and quantify mast cells basophils a murine asthma model, determine if both were increased by multiple antigen challenges assess roles those asthmatic responses.Sensitized Balb/c intratracheally challenged ovalbumin four times. enzymatically digested lung tissue detected flow cytometry. An anti-FcεRI monoclonal antibody, MAR-1, was...
The intermediate-conductance Ca(2+)-activated K(+) channel KCa3.1/KCNN4 plays an important role in the modulation of Ca(2+) signaling through control membrane potential T lymphocytes. Here, we study involvement KCa3.1 enlargement mesenteric lymph nodes (MLNs) a mouse model inflammatory bowel disease (IBD). IBD was prepared by exposing male C57BL/6J mice to 5% dextran sulfate sodium for 7 days. Inflammation-induced changes activity and expressions its regulators MLN CD4(+) lymphocytes were...
Heme proteins play diverse and important biological roles, from electron transfer chemical catalysis to oxygen transport and/or storage. Although the distortion of heme porphyrin correlates with physical properties heme, such as redox potential affinity, relationship between protein environment is unclear. Here, we tested hypothesis that heme-binding pocket determines (conformation). We analyzed correlations amino acid composition magnitude along 12 vibrational modes using machine learning....
Abstract: Dry skin/barrier dysfunction is considered to be one of the characteristic features atopic dermatitis (AD). When HR‐1 hairless mice are fed a special diet, HR‐AD, dry red skin induced. We examined whether HR‐AD–fed mouse could used as model for AD by showing itch‐associated scratching behaviour and analysing immunological change. were HR‐AD from 4 weeks old. showed severe symptoms accompanied decrease in dermal water content an increase transepidermal loss prolonged bout duration...
The present studies were carried out to investigate the effect of several growth factors on human endometrial stromal cells. In cells, bombesin and bradykinin provoked an increase in intracellular free Ca2+ labelled inositol phosphates when pre-incubated with [3H]myoinositol. Some or possibly all initial represented a mobilization from stores second phase response depended influx extracellular medium. [3H]Thymidine was added cultured cells bombesin, bradykinin, epidermal factor (EGF),...
Hairless mice fed with a special diet (named HR-AD) show atopic dermatitis (AD)-like pruritic skin inflammation that is almost completely resolved the supplementation of an unsaturated fatty acid (UFA), linoleic (LA). This suggests dietary deficiency LA key cause this dermatitis. However, because there no appropriate control for HR-AD, involvement other ingredients cannot be ruled out. Furthermore, it has not yet been tested whether only UFA can such AD-like pruritus. In study, using...
The expression and functional role of CysLT2 receptors in asthma have not been clarified. In this study, we evaluated expression, effects CysLT2-and CysLT1/2-receptor antagonists on antigen-induced bronchoconstriction using isolated lung tissues from both non-asthma subjects.CysLT1 tissue preparations was examined immunohistochemistry experiments, their roles were assessed ONO-6950, a dual antagonist, montelukast, CysLT1 receptor BayCysLT2RA, receptor-specific antagonist.CysLT1 expressed the...
Background The vascular NAD(P)H oxidase-derived superoxide anion (O2-) plays a crucial role in the pathological progression of hypertension and atherosclerosis, HMG-CoA reductase inhibitors (statins) have antioxidant effects. However, it is unclear whether oxidase involved endothelial dysfunction congestive heart failure (CHF) exert their vasoprotective effects CHF. present study examined both involvement dogs with tachycardia-induced CHF therapeutic effect statin (pitavastatin). Methods...